Photocatalytic antimicrobial activity of thin surface films of TiO2, CuO and TiO2 /CuO dual layers on Escherichia coli and bacteriophage T4

Ditta, IB; Steele, A; Liptrott, C; Tobin, J; Tyler, H; Yates, HM; Sheel, DW; Foster, HA

Abstract

TiO2 coated surfaces are increasingly studied for their ability to inactivate microorganisms. The activity of glass coated with thin films of TiO2, CuO and hybrid CuO/TiO2 prepared by atmospheric Chemical Vapour Deposition (Ap-CVD) and TiO2 prepared by a sol-gel process was investigated using the inactivation of bacteriophage T4 as a model for
inactivation of viruses. The chemical oxidising activity was also determined by measuring stearic acid oxidation. The results showed that the rate of inactivation of bacteriophage T4 increased with increasing chemical oxidising activity with the maximum rate obtained on
highly active sol-gel preparations. However these were delicate and easily damaged unlike the Ap-CVD coatings. Inactivation rates were highest on CuO and CuO/TiO2 which had the lowest chemical oxidising activities. The inactivation of T4 was higher than that of Escherichia coli on low activity surfaces. The combination of photocatalysis and toxicity of copper acted synergistically to inactivate bacteriophage T4 and retained some selfcleaning activity. The presence of phosphate ions slowed inactivation but NaCl had no effect. The results show that TiO2/CuO coated surfaces are highly antiviral and may have applications in the food and healthcare industries.

Citation

Ditta, I., Steele, A., Liptrott, C., Tobin, J., Tyler, H., Yates, H., …Foster, H. (2008). Photocatalytic antimicrobial activity of thin surface films of TiO2, CuO and TiO2 /CuO dual layers on Escherichia coli and bacteriophage T4. Applied Microbiology and Biotechnology, 79(1), 127-133. https://doi.org/10.1007/s00253-008-1411-8

• Journal Article Type: Article
• Publication Date: Jan 1, 2008
• Deposit Date: Oct 21, 2011
• Publicly Available Date: Oct 21, 2011
• Journal: Applied Microbiology and Biotechnology
• Print ISSN: 0175-7598
• Publisher: Springer Verlag
• Peer Reviewed: Not Peer Reviewed
• Volume: 79
• Issue: 1
• Pages: 127-133
• DOI: https://doi.org/10.1007/s00253-008-1411-8
• Keywords: Antimicrobial, bacteriophage T4, CVD, Escherichia coli, photocatalysis, surfaces, TiO2
• Publisher URL: http://dx.doi.org/10.1007/s00253-008-1411-8