Rapid dot-ELISA for the detection of specific antigens in the cyst fluid from human cases of cystic echinococcosis.

Wang, Y; Bradshaw, H; Rogan, MT; Craig, PS

Abstract

A rapid dot-ELISA was developed for the detection of specific antigens in samples of cyst fluid from human cases of Echinococcus granulosus infection, permitting the confirmation of cystic echinococcosis (CE). Peroxidase-conjugated antibodies against antigen B (derived from the fluid in the cysts of sheep infected with E. granulosus) were used to test cyst-fluid samples from 22, surgically confirmed cases of human CE and 21 domestic animals (horses, sheep, buffalo, a cow and a camel) with CE. All of these samples were found to be strongly positive in the dot-ELISA, by direct reading with the naked eye. In contrast, fluid samples from seven, non-parasitic liver cysts of human origin were all negative, and a sample taken from the residual cavity left in a patient after previous CE surgery showed a weakly positive reaction. The antigen-detection assay, which can be completed within 10min, may be a useful 'bedside' test for the differential diagnosis of cysts during surgery or percutaneous treatment for suspected CE.

Citation

Wang, Y., Bradshaw, H., Rogan, M., & Craig, P. (2002). Rapid dot-ELISA for the detection of specific antigens in the cyst fluid from human cases of cystic echinococcosis. Annals of Tropical Medicine and Parasitology, 96(7), 691-694. https://doi.org/10.1179/000349802125001717

• Journal Article Type: Article
• Publication Date: Oct 1, 2002
• Deposit Date: Aug 10, 2007
• Journal: Annals of Tropical Medicine and Parasitology
• Print ISSN: 0003-4983
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 96
• Issue: 7
• Pages: 691-694
• DOI: https://doi.org/10.1179/000349802125001717