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Development and evaluation of loop-mediated isothermal amplification (LAMP) for rapid detection of Clonorchis sinensis from its first intermediate hosts, freshwater snails.

Y, Chen; T, Wen; DH, Lai; YZ, Wen; ZD, Wu; TB, Yang; XB, Yu; G, Hide; ZR, Lun

Authors

Chen Y

Wen T

Lai DH

Wen YZ

Wu ZD

Yang TB

Yu XB

Lun ZR



Abstract

Clonorchiasis, caused by Clonorchis sinensis, is a key foodborne zoonosis, which is mainly found in China, Korea and Vietnam. Detection of this parasite from the second intermediate host, the freshwater fish is the common method for epidemiological surveys of this parasite, but is time consuming, labour intensive and easily leads to misdiagnosis. In this study, we have developed a rapid, sensitive and reliable molecular method for the diagnosis of C. sinensis from its first intermediate hosts, freshwater snails, based on a loop-mediated isothermal amplification (LAMP) method. The specific amplified fragment from genomic DNA of C. sinensis did not cross-react with those from other relevant trematodes and a range of hosts (freshwater fish, shrimps and snails) of C. sinensis living in similar environments. The detection limit of the LAMP method was as low as 10 fg which was 1000 times more sensitive than conventional PCR, which was also demonstrated by successful application to field samples. These results show that the LAMP method is a more sensitive tool than conventional PCR for the detection of C. sinensis infection in the first intermediate hosts and, due to a simpler protocol, is an ideal molecular method for field-based epidemiological surveys of this parasite.

Citation

Y, C., T, W., DH, L., YZ, W., ZD, W., TB, Y., …ZR, L. (2013). Development and evaluation of loop-mediated isothermal amplification (LAMP) for rapid detection of Clonorchis sinensis from its first intermediate hosts, freshwater snails. Parasitology, 140(11), https://doi.org/10.1017/S0031182013000498

Journal Article Type Article
Online Publication Date Jul 22, 2013
Publication Date 2013-09
Deposit Date Mar 15, 2024
Print ISSN 0031-1820
Publisher Cambridge University Press (CUP)
Peer Reviewed Peer Reviewed
Volume 140
Issue 11
DOI https://doi.org/10.1017/S0031182013000498
PMID 23870065