Dr Amy Morgan A.E.Morgan2@salford.ac.uk
Lecturer
Dr Amy Morgan A.E.Morgan2@salford.ac.uk
Lecturer
J Enrique Salcedo-Sora
Dr Mark McAuley M.McAuley@salford.ac.uk
Lecturer
Folate (vitamin B9) plays a central role in one-carbon metabolism in prokaryotes and eukaryotes. This pathway mediates the transfer of one-carbon units, playing a crucial role in nucleotide synthesis, methylation, and amino acid homeostasis. The folinic acid futile cycle adds a layer of intrigue to this pathway, due to its associations with metabolism, cell growth, and dormancy. It also introduces additional complexity to folate metabolism. A logical way to deal with such complexity is to examine it by using mathematical modelling. This work describes the construction and analysis of a model of folate metabolism, which includes the folinic acid futile cycle. This model was tested under three in silico growth conditions. Model simulations revealed: 1) the folate cycle behaved as a stable biochemical system in three growth states (slow, standard, and rapid); 2) the initial concentration of serine had the greatest impact on metabolite concentrations; 3) 5-formyltetrahydrofolate cyclo-ligase (5-FCL) activity had a significant impact on the levels of the 7 products that carry the one-carbon donated from folates, and the redox couple NADP/NADPH; this was particularly evident in the rapid growth state; 4) 5-FCL may be vital to the survival of the cells by maintaining low levels of homocysteine, as high levels can induce toxicity; and 5) the antifolate therapeutic trimethoprim had a greater impact on folate metabolism with higher nutrient availability. These results highlight the important role of 5-FCL in intracellular folate homeostasis and mass generation under different metabolic scenarios. [Abstract copyright: Copyright © 2023 Elsevier B.V. All rights reserved.]
Morgan, A., Salcedo-Sora, J. E., & Mc Auley, M. T. (2024). A new mathematical model of folate homeostasis in E. coli highlights the potential importance of the folinic acid futile cycle in cell growth. BioSystems, 235, 105088. https://doi.org/10.1016/j.biosystems.2023.105088
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 17, 2023 |
Online Publication Date | Nov 23, 2023 |
Publication Date | 2024-01 |
Deposit Date | Dec 13, 2023 |
Publicly Available Date | Dec 13, 2023 |
Journal | Biosystems |
Print ISSN | 0303-2647 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 235 |
Pages | 105088 |
DOI | https://doi.org/10.1016/j.biosystems.2023.105088 |
Keywords | Futile cycle, Cell dormancy, Folinic acid, Bacteria, Mathematical model, Cell growth |
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