Delayed feeding disrupts diurnal oscillations in the gut microbiome of a neotropical bat in captivity

Melville, Dominik W; Meyer, Magdalena; Kümmerle, Corbinian; Alvarado-Barrantes, Kevin A; Wilhelm, Kerstin; Sommer, Simone; Tschapka, Marco; Risely, Alice

Abstract

Diurnal rhythms of the gut microbiota are emerging as an important yet often overlooked facet of microbial ecology. Feeding is thought to stimulate gut microbial rhythmicity, but this has not been explicitly tested. Moreover, the role of the gut environment is entirely unexplored, with rhythmic changes to gut pH rather than feeding per se possibly affecting gut microbial fluctuations. In this study, we experimentally manipulated the feeding schedule of captive lesser long-nosed bats, Leptonycteris yerbabuenae, to dissociate photic and feeding cues, and measured the faecal microbiota and gut pH every 2 h. We detected strong diurnal rhythms in both microbial alpha diversity and beta diversity as well as in pH within the control group. However, a delay in feeding disrupted oscillations of gut microbial diversity and composition, but did not affect rhythms in gut pH. The oscillations of some genera, such as Streptococcus, which aid in metabolizing nutrients, shifted in accordance with the delayed-feeding cue and were correlated with pH. For other bacterial genera, oscillations were disturbed and no connection to pH was found. Our findings suggest that the rhythmic proliferation of bacteria matches peak feeding times, providing evidence that diurnal rhythms of the gut microbiota likely evolved to optimize their metabolic support to the host’s circadian phenotype.

• Journal Article Type: Article
• Acceptance Date: Jan 21, 2025
• Online Publication Date: Jan 22, 2025
• Publication Date: Jan 22, 2025
• Deposit Date: Feb 28, 2025
• Publicly Available Date: Feb 28, 2025
• Journal: FEMS Microbiology Ecology
• Print ISSN: 0168-6496
• Electronic ISSN: 1574-6941
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 101
• Issue: 2
• Article Number: fiaf012
• DOI: https://doi.org/10.1093/femsec/fiaf012
• Keywords: Leptonycteris yerbabuenae, diet experiment, gut pH, circadian rhythm, dysbiosis, microbial ecology