Bacteria transformation, Mini prep and PCR analysis

Morgan, Nicky; Osifo, Tosin

Abstract

Bacterial transformation is a useful tool in molecular genetics, being used to create genetically modified bacteria capable of adapting and surviving in conditions that the parent culture would not. Introducing a plasmid into a bacterial host can be a difficult procedure, requiring molecular techniques. The materials and methods provided give an overall instruction on how to prepare and proceed with mini prepping bacterial samples from pellets, carrying out a PCR on purified DNA and the bacterial transformation and gene cloning. The following explains the volumes used in this procedure along with the cells and consumables used.

• Other Type: Protocol
• Online Publication Date: Jun 19, 2025
• Publication Date: Jun 19, 2025
• Deposit Date: Jun 23, 2025
• Publicly Available Date: Jun 24, 2025
• DOI: https://doi.org/10.25416/NTR.29352698.v1