All Outputs (31)

The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca(2+) handling in rat ventricular myocytes (2015)
Journal Article
Miller, L., Greensmith, D., Sankaranarayanan, R., O'Neill, S., & Eisner, D. (2015). The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca(2+) handling in rat ventricular myocytes. Cell Calcium, 58(2), 208-14. https://doi.org/10.1016/j.ceca.2015.05.002

2,5-Di-(tert-butyl)-1,4-benzohydroquinone (TBQ) is a reversible inhibitor of SERCA, potentially making it a useful tool to study the effects of SERCA inhibition in cardiac cells. However, it is unknown if TBQ also has effects on other components of v... Read More about The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca(2+) handling in rat ventricular myocytes.

Perturbed atrial calcium handling in an ovine model of heart failure : potential roles for reductions in the L-type calcium current (2014)
Journal Article

Heart failure (HF) is commonly associated with reduced cardiac output and an increased risk of atrial arrhythmias particularly during β-adrenergic stimulation. The aim of the present study was to determine how HF alters systolic Ca(2+) and the respon... Read More about Perturbed atrial calcium handling in an ovine model of heart failure : potential roles for reductions in the L-type calcium current.

Longstanding complex regional pain syndrome is associated with activating autoantibodies against alpha-1a adrenoceptors (2014)
Journal Article

Complex regional pain syndrome (CRPS) is a limb-confined posttraumatic pain syndrome with sympathetic features. The cause is unknown, but the results of a randomized crossover trial on low-dose intravenous immunoglobulins (IVIG) treatment point to a... Read More about Longstanding complex regional pain syndrome is associated with activating autoantibodies against alpha-1a adrenoceptors.

The effects of 2',5'-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes (2014)
Journal Article
Greensmith, D., Miller, L., & Eisner, D. (2014). The effects of 2',5'-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes. Proceedings of the Physiological Society (Online), 31, PCA042

2',5'-di(tert-butyl)-1,4-benzohydroquinone (TBQ) is a reversible inhibitor of SERCA, potentially making it a useful tool to study the effects of SERCA inhibition. It has been used in a variety of cell types including ventricular myocytes (1). However... Read More about The effects of 2',5'-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes.

Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions (2014)
Journal Article
Greensmith, D., Galli, G., Trafford, A., & Eisner, D. (2014). Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions. Cardiovascular Research, 103(4), 554-563. https://doi.org/10.1093/cvr/cvu158

AIMS: Most of the calcium that activates contraction is released from the sarcoplasmic reticulum (SR) through the ryanodine receptor (RyR). It is controversial whether activators of the RyR produce a maintained increase in the amplitude of the systol... Read More about Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions.

Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis (2013)
Journal Article
Greensmith, D. (2014). Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis. Computer Methods and Programs in Biomedicine, 113(1), 241-250. https://doi.org/10.1016/j.cmpb.2013.09.004

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological info... Read More about Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.

The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes (2013)
Journal Article
Greensmith, D., & Nirmalan, M. (2013). The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes. Physiological Reports, 1(4), e00093. https://doi.org/10.1002/phy2.93

The proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) is associated with myocardial dysfunction observed in sepsis and septic shock. There are two fundamental components to this dysfunction. (1) systolic dysfunction; and (2) diastolic dysf... Read More about The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes.

Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation (2013)
Journal Article

RATIONALE

Spontaneous Ca(2+) release (SCR) from the sarcoplasmic reticulum can cause delayed afterdepolarizations and triggered activity, contributing to arrhythmogenesis during β-adrenergic stimulation. Excessive beat-to-beat variability of repol... Read More about Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation.

Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged (2012)
Journal Article

The incidence of heart failure (HF) increases with age. This study sought to determine whether aging exacerbates structural and functional remodeling of the myocardium in HF. HF was induced in young (~18 months) and aged sheep (>8 years) by right ven... Read More about Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged.

Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure (2011)
Journal Article

Reduced inotropic responsiveness is characteristic of heart failure (HF). This study determined the cellular Ca2+ homeostatic and molecular mechanisms causing the blunted β-adrenergic (β-AR) response in HF.We induced HF by tachypacing in sheep; intra... Read More about Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure.

The effects of hydrogen peroxide on intracellular calcium handling and contractility in the rat ventricular myocyte (2010)
Journal Article
Greensmith, D., Eisner, D., & Nirmalan, M. (2010). The effects of hydrogen peroxide on intracellular calcium handling and contractility in the rat ventricular myocyte. Cell Calcium, 48(6), 341-51. https://doi.org/10.1016/j.ceca.2010.10.007

Elevations in reactive oxygen species are implicated in many disease states and cause systolic and diastolic myocardial dysfunction. To understand the underlying cellular dysfunction, we characterised the effects of H₂O₂ on [Ca(2+)](i) handling and c... Read More about The effects of hydrogen peroxide on intracellular calcium handling and contractility in the rat ventricular myocyte.