All Outputs (14)

Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding (2020)
Journal Article
Sales, N., Wangensteen Fuentes, O., Carvalho, D., Deiner, K., Praebel, K., Coscia, I., …Mariani, S. (2021). Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding. Science of the Total Environment, 754, 142096. https://doi.org/10.1016/j.scitotenv.2020.142096

The biodiverse Neotropical ecoregion remains insufficiently assessed, poorly managed, and threatened by unregulated human activities. Novel, rapid and cost-effective DNA-based approaches are valuable to improve understanding of the biological communi... Read More about Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding.

Fishing for mammals : landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems (2020)
Journal Article
Sales, N., McKenzie, M., Drake, J., Harper, L., Browett, S., Coscia, I., …McDevitt, A. (2020). Fishing for mammals : landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems. Journal of Applied Ecology, 57(4), 707-716. https://doi.org/10.1111/1365-2664.13592

1. Environmental DNA (eDNA) metabarcoding has revolutionised biomonitoring in both marine and freshwater ecosystems. However, for semi-aquatic and terrestrial animals, the application of this technique remains relatively untested. 2. We first ass... Read More about Fishing for mammals : landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems.

Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding (2019)
Journal Article
Bakker, J., Wangensteen Fuentes, O., Baillie, C., Buddo, D., Chapman, D., Gallagher, A., …Mariani, S. (2019). Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding. Ecology and Evolution, 9(24), 14341-14355. https://doi.org/10.1002/ece3.5871

Our understanding of marine communities and their functions in an ecosystem relies on the ability to detect and monitor species distributions and abundances. Currently, the use of environmental DNA (eDNA) metabarcoding is increasingly being applied f... Read More about Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding.

Non‐specific amplification compromises environmental DNA metabarcoding with COI (2019)
Journal Article
Collins, R., Bakker, J., Wangensteen Fuentes, O., Soto, A., Corrigan, L., Sims, D., …Mariani, S. (2019). Non‐specific amplification compromises environmental DNA metabarcoding with COI. Methods in Ecology and Evolution, 10(11), 1985-2001. https://doi.org/10.1111/2041-210X.13276

1.Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, and especially so when developing community standar... Read More about Non‐specific amplification compromises environmental DNA metabarcoding with COI.

Environmental DNA metabarcoding as an effective and rapid tool for fish monitoring in canals (2019)
Journal Article
McDevitt, A., Sales, N., Browett, S., Sparnenn, A., Mariani, S., Wangensteen Fuentes, O., …Benvenuto, C. (2019). Environmental DNA metabarcoding as an effective and rapid tool for fish monitoring in canals. Journal of Fish Biology, 95(2), 679-682. https://doi.org/10.1111/jfb.14053

We focus on a case study along an English canal comparing environmental DNA (eDNA) metabarcoding with two types of electrofishing techniques (wade‐and‐reach and boom‐boat). In addition to corroborating data obtained by electrofishing, eDNA provided a... Read More about Environmental DNA metabarcoding as an effective and rapid tool for fish monitoring in canals.

Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river (2019)
Journal Article
Sales, N., Wangensteen Fuentes, O., Carvalho, D., & Mariani, S. (2019). Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river. Environmental DNA, 1(2), 119-130. https://doi.org/10.1002/edn3.14

Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limit... Read More about Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river.

Persistence of environmental DNA in marine systems (2018)
Journal Article
Collins, R., Wangensteen Fuentes, O., O'Gorman, E., Mariani, S., Sims, D., & Genner, M. (2018). Persistence of environmental DNA in marine systems. Communications Biology, 1, 185. https://doi.org/10.1038/s42003-018-0192-6

As environmental DNA (eDNA) becomes an increasingly valuable resource for marine eco-system monitoring, understanding variation in its persistence across contrasting environments is critical. Here, we quantify the breakdown of macrobial eDNA over a s... Read More about Persistence of environmental DNA in marine systems.

Metabarcoding of shrimp stomach content: harnessing a natural sampler for fish biodiversity monitoring (2018)
Journal Article
Siegenthaler, A., Wangensteen, O., Soto, A., Benvenuto, C., Corrigan, L., & Mariani, S. (2019). Metabarcoding of shrimp stomach content: harnessing a natural sampler for fish biodiversity monitoring. Molecular Ecology Resources, 19(1), 206-220. https://doi.org/10.1111/1755-0998.12956

Given their positioning and biological productivity, estuaries have long represented key providers of ecosystem services, and consequently remain under remarkable pressure from numerous forms of anthropogenic impact. The monitoring of fish communitie... Read More about Metabarcoding of shrimp stomach content: harnessing a natural sampler for fish biodiversity monitoring.

DNA metabarcoding unveils multi-scale trophic variation in a widespread coastal opportunist (2018)
Journal Article
Siegenthaler, A., Wangensteen, O., Benvenuto, C., Campos, J., & Mariani, S. (2019). DNA metabarcoding unveils multi-scale trophic variation in a widespread coastal opportunist. Molecular Ecology, 28(2), 232-249. https://doi.org/10.1111/mec.14886

A thorough understanding of ecological networks relies on comprehensive information on trophic relationships among species. Since unpicking the diet of many organisms is unattainable using traditional morphology-based approaches, the application of h... Read More about DNA metabarcoding unveils multi-scale trophic variation in a widespread coastal opportunist.

Environmental DNA illuminates the dark diversity of sharks (2018)
Journal Article
Boussarie, G., Bakker, J., Wangensteen Fuentes, O., Mariani, S., Bonnin, L., Juhel, J., …Mouillot, D. (2018). Environmental DNA illuminates the dark diversity of sharks. Science Advances, 4(5), eaap9661. https://doi.org/10.1126/sciadv.aap9661

In the era of "Anthropocene defaunation," large species are often no longer detected in habitats where they formerly occurred. However, it is unclear whether this apparent missing, or "dark," diversity of megafauna results from local species extirpat... Read More about Environmental DNA illuminates the dark diversity of sharks.

Under the canopy : community-wide effects of invasive algae in marine protected areas revealed by metabarcoding (2017)
Journal Article
Wangensteen, O., Cebrian, E., Palacín, C., & Turon, X. (2018). Under the canopy : community-wide effects of invasive algae in marine protected areas revealed by metabarcoding. Marine Pollution Bulletin, 127, 54-66. https://doi.org/10.1016/j.marpolbul.2017.11.033

We analysed with multigene (18S and COI) metabarcoding the effects of the proliferation of invasive seaweeds on rocky littoral communities in two Spanish Marine Protected Areas. The invasive algae studied were Caulerpa cylindracea, Lophocladia lallem... Read More about Under the canopy : community-wide effects of invasive algae in marine protected areas revealed by metabarcoding.

Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact (2017)
Journal Article
Bakker, J., Wangensteen, O., Chapman, D., Boussarie, G., Boudo, D., Guttridge, T., …Mariani, S. (2017). Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact. Scientific reports, 7, No. 16886. https://doi.org/10.1038/s41598-017-17150-2

Sharks are charismatic predators that play a key role in most marine food webs. Their demonstrated vulnerability to exploitation has recently turned them into flagship species in ocean conservation. Yet, the assessment and monitoring of the distribut... Read More about Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact.

Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA (2016)
Journal Article
Guardiola, M., Wangensteen Fuentes, O., Taberlet, P., Coissac, E., Uriz, M., & Turon, X. (2016). Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA. PeerJ, 4, e2807. https://doi.org/10.7717/peerj.2807

We assessed spatio-temporal patterns of diversity in deep-sea sediment communities using metabarcoding. We chose a recently developed eukaryotic marker based on the v7 region of the 18S rRNA gene. Our study was performed in a submarine canyon and its... Read More about Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA.

Hope springs eternal in the starfish gonad: preserved potential for sexual reproduction in a single-clone population of a fissiparous starfish (2016)
Journal Article
Garcia-Cisneros, A., Pérez-Portela, R., Wangensteen Fuentes, O., Campos-Canet, M., & Palacín, C. (2017). Hope springs eternal in the starfish gonad: preserved potential for sexual reproduction in a single-clone population of a fissiparous starfish. Hydrobiologia, 787(1), 291-305. https://doi.org/10.1007/s10750-016-2971-8

Among echinoderms, asexual reproduction by fission occurs in few species. This strategy is considered a temporary response to stressful conditions and usually alternates with sexual reproduction events; thus, monoclonal populations are extremely rare... Read More about Hope springs eternal in the starfish gonad: preserved potential for sexual reproduction in a single-clone population of a fissiparous starfish.