Optimization of SPIO injection for sentinel lymph node dissection in a rat model

Peek, MCL; Saeki, K; Ohashi, K; Chikaki, S; Baker, RD; Nakagawa, T; Kusakabe, M; Douek, M; Sekino, M

Abstract

The magnetic technique, consisting of a magnetic tracer and a handheld magnetometer, is a promising alternative technique for sentinel lymph node dissection (SLND) and was shown to be non-inferior to the standard technique in terms of identification rates. In this study, injection characteristics (iron dose, dilution, time course and massaging) were evaluated to optimize magnetic tracer uptake in the sentinel lymph nodes (SLN) in a rat hindleg model. 202 successful SLNDs were performed. Iron uptake in the SLN is proportional (10% utilization rate) to the injection dose between 20 and 200 μg, showing a plateau uptake of 80 μg in the SLN around 1,000 μg injection. Linear regression showed that time had a higher impact than dilution, on the SLN iron uptake. Massaging showed no significant change in iron uptake. The amount of residual iron at the injection site was also proportional to the injection dose without any plateau. Time was a significant factor for wash-out of residual iron. From these results, preoperative injection may be advantageous for SLN detection as well as reduction in residual iron at the injection site by potential decrease in required injection dose.

Citation

Peek, M., Saeki, K., Ohashi, K., Chikaki, S., Baker, R., Nakagawa, T., …Sekino, M. (2021). Optimization of SPIO injection for sentinel lymph node dissection in a rat model. Cancers, 13(19), e5031. https://doi.org/10.3390/cancers13195031

• Journal Article Type: Article
• Acceptance Date: Oct 5, 2021
• Publication Date: Oct 8, 2021
• Deposit Date: Oct 12, 2021
• Publicly Available Date: Oct 12, 2021
• Journal: Cancers
• Publisher: MDPI
• Volume: 13
• Issue: 19
• Pages: e5031
• DOI: https://doi.org/10.3390/cancers13195031
• Publisher URL: https://doi.org/10.3390/cancers13195031
• Related Public URLs: https://www.mdpi.com/journal/cancers
• Additional Information: Additional Information : ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2072-6694 **History: published 08-10-2021; accepted 05-10-2021; Funders : Japan Agency for Medical Research and Development;JSPS KAKENHI; Grant Number: 18he0902010h0004; Grant Number: 20H05759