C Guilding
A riot of rhythms : neuronal and glial circadian oscillators in the mediobasal hypothalamus
Guilding, C; Hughes, ATL; Brown, TM; Namvar, S; Piggins, HD
Authors
Abstract
Background: In mammals, the synchronized activity of cell autonomous clocks in the suprachiasmatic nuclei (SCN) enables this structure to function as the master circadian clock, coordinating daily rhythms in physiology and behavior. However, the dominance of this clock has been challenged by the observations that metabolic duress can over-ride SCN controlled rhythms, and that clock genes are expressed in many brain areas, including those implicated in the regulation of appetite and feeding. The recent development of mice in which clock gene/protein activity is reported by bioluminescent constructs (luciferase or luc) now enables us to track molecular oscillations in numerous tissues ex vivo. Consequently we determined both clock activities and responsiveness to metabolic perturbations of cells and tissues within the mediobasal hypothalamus (MBH), a site pivotal for optimal internal homeostatic regulation.
Results: Here we demonstrate endogenous circadian rhythms of PER2::LUC expression in discrete subdivisions of the arcuate (Arc) and dorsomedial nuclei (DMH). Rhythms resolved to single cells did not maintain long-term synchrony with one-another, leading to a damping of oscillations at both cell and tissue levels. Complementary electrophysiology recordings revealed rhythms in neuronal activity in the Arc and DMH. Further, PER2::LUC rhythms were detected in the ependymal layer of the third ventricle and in the median eminence/pars tuberalis (ME/PT). A high-fat diet had no effect on the molecular oscillations in the MBH, whereas food deprivation resulted in an altered phase in the ME/PT.
Conclusion: Our results provide the first single cell resolution of endogenous circadian rhythms in clock gene expression in any intact tissue outside the SCN, reveal the cellular basis for tissue level damping in extra-SCN oscillators and demonstrate that an oscillator in the ME/PT is responsive to changes in metabolism. © 2009 Guilding et al.
Citation
Guilding, C., Hughes, A., Brown, T., Namvar, S., & Piggins, H. (2009). A riot of rhythms : neuronal and glial circadian oscillators in the mediobasal hypothalamus. Molecular Brain, 2(28), https://doi.org/10.1186/1756-6606-2-28
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 27, 2009 |
Online Publication Date | Aug 27, 2009 |
Publication Date | Aug 27, 2009 |
Deposit Date | Mar 29, 2019 |
Publicly Available Date | Mar 29, 2019 |
Journal | Molecular Brain |
Publisher | Springer Verlag |
Volume | 2 |
Issue | 28 |
DOI | https://doi.org/10.1186/1756-6606-2-28 |
Publisher URL | https://doi.org/10.1186/1756-6606-2-28 |
Related Public URLs | https://molecularbrain.biomedcentral.com/ |
Files
Namvar 2009.pdf
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Licence
http://creativecommons.org/licenses/by/3.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/3.0/
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