Magnetic sentinel lymph node biopsy in a murine tumour model

Ahmed, M; Woo, T; Ohashi, K; Suzuki, T; Kaneko, A; Hoshino, A; Zada, A; Baker, RD; Douek, M; Kusakabe, M; Sekino, M

Abstract

The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement
to reduce “residual iron content” at injection sites by maximising lymphatic uptake to prevent “void artefacts” on magnetic resonance
imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine
tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and
assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed.
Rapid iron uptake on MRI, smaller “void artefacts”(P < 0.001) and a significant increase in iron content with time were identified in the
subcutaneous injection group (r = 0.937; P < 0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is
beneficial for lymphatic iron uptake.

Citation

Ahmed, M., Woo, T., Ohashi, K., Suzuki, T., Kaneko, A., Hoshino, A., …Sekino, M. (2015). Magnetic sentinel lymph node biopsy in a murine tumour model. Nanomedicine: Nanotechnology, Biology and Medicine, 12(4), 1045-1052. https://doi.org/10.1016/j.nano.2015.12.366

• Journal Article Type: Article
• Acceptance Date: Dec 10, 2015
• Online Publication Date: Dec 28, 2015
• Publication Date: Dec 28, 2015
• Deposit Date: Apr 13, 2016
• Journal: Nanomedicine: Nanotechnology, Biology and Medicine
• Print ISSN: 1549-9634
• Publisher: Elsevier
• Volume: 12
• Issue: 4
• Pages: 1045-1052
• DOI: https://doi.org/10.1016/j.nano.2015.12.366
• Publisher URL: http://dx.doi.org/10.1016/j.nano.2015.12.366
• Related Public URLs: http://www.journals.elsevier.com/nanomedicine-nanotechnology-biology-and-medicine/