Diversity in a honey bee pathogen: first report of a third master variant of the Deformed Wing Virus quasispecies

Mordecai, Gideon J; Wilfert, Lena; Martin, Stephen J; Jones, Ian M; Schroeder, Declan C

Abstract

Treatment of emerging RNA viruses is hampered by the high mutation and replication rates that enable these viruses to operate as a quasispecies. Declining honey bee populations have been attributed to the ectoparasitic mite Varroa destructor and its affiliation with Deformed Wing Virus (DWV). In the current study we use next-generation sequencing to investigate the DWV quasispecies in an apiary known to suffer from overwintering colony losses. We show that the DWV species complex is made up of three master variants. Our results indicate that a new DWV Type C variant is distinct from the previously described types A and B, but together they form a distinct clade compared with other members of the Iflaviridae. The molecular clock estimation predicts that Type C diverged from the other variants ∼319 years ago. The discovery of a new master variant of DWV has important implications for the positive identification of the true pathogen within global honey bee populations.

Citation

Mordecai, G. J., Wilfert, L., Martin, S. J., Jones, I. M., & Schroeder, D. C. (2016). Diversity in a honey bee pathogen: first report of a third master variant of the Deformed Wing Virus quasispecies. ISME Journal, 10(5), 1264-1273. https://doi.org/10.1038/ismej.2015.178

• Journal Article Type: Article
• Acceptance Date: Aug 21, 2015
• Online Publication Date: Nov 17, 2015
• Publication Date: May 1, 2016
• Deposit Date: Dec 1, 2015
• Publicly Available Date: Oct 18, 2018
• Journal: The ISME Journal
• Print ISSN: 1751-7362
• Electronic ISSN: 1751-7370
• Publisher: Nature Publishing Group
• Volume: 10
• Issue: 5
• Pages: 1264-1273
• DOI: https://doi.org/10.1038/ismej.2015.178
• Publisher URL: http://dx.doi.org/10.1038/ismej.2015.178
• Related Public URLs: http://www.nature.com/ismej/index.html