NE IIott
Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide-induced inflammatory response in human monocytes
IIott, NE; Heward, JA; Roux, B; Tsitsiou, E; Fenwick, PS; Lenzi, L; Goodhead, IB; Hertz-Fowler, C; Heger, A; Hall, N; Donnelly, LE; Sims, D; Lindsay, MA
Authors
JA Heward
B Roux
E Tsitsiou
PS Fenwick
L Lenzi
Prof Ian Goodhead I.B.Goodhead@salford.ac.uk
Associate Dean Research & Innovation
C Hertz-Fowler
A Heger
N Hall
LE Donnelly
D Sims
MA Lindsay
Abstract
Early reports indicate that long non-coding RNAs (lncRNAs) are novel regulators of biological responses. However, their role in the human innate immune response, which provides the initial defence against infection, is largely unexplored. To address this issue, here we characterize the long non-coding RNA transcriptome in primary human monocytes using RNA sequencing. We identify 76 enhancer RNAs (eRNAs), 40 canonical lncRNAs, 65 antisense lncRNAs and 35 regions of bidirectional transcription (RBT) that are differentially expressed in response to bacterial lipopolysaccharide (LPS). Crucially, we demonstrate that knockdown of nuclear-localized, NF-κB-regulated, eRNAs (IL1β-eRNA) and RBT (IL1β-RBT46) surrounding the IL1β locus, attenuates LPS-induced messenger RNA transcription and release of the proinflammatory mediators, IL1β and CXCL8. We predict that lncRNAs can be important regulators of the human innate immune response.
Citation
IIott, N., Heward, J., Roux, B., Tsitsiou, E., Fenwick, P., Lenzi, L., …Lindsay, M. (2014). Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide-induced inflammatory response in human monocytes. Nature communications, 5, 3979-3979. https://doi.org/10.1038/ncomms4979
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 29, 2014 |
Publication Date | Jun 9, 2014 |
Deposit Date | Sep 23, 2015 |
Publicly Available Date | Apr 5, 2016 |
Journal | Nature Communications |
Print ISSN | 2041-1723 |
Electronic ISSN | 2041-1723 |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Pages | 3979-3979 |
DOI | https://doi.org/10.1038/ncomms4979 |
Publisher URL | http://dx.doi.org/10.1038/ncomms4979 |
Related Public URLs | http://www.nature.com/ncomms/index.html |
Additional Information | Funders : Biotechnology and Biosciences Sciences Research Council (BBSRC);Medical Research Council (MRC) |
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Licence
http://creativecommons.org/licenses/by/3.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/3.0/
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