J Shakeri
Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects
Shakeri, J; Foster, HA
Authors
HA Foster
Abstract
Two strains of Trichoderma harzianum, 101645, an insect pathogen and 206040, used for biological control of fungal plant pathogens were investigated for the production of serine protease, chitinase and antibiotic activity in relation to entomopathogenicity. Both strains produced serine protease with a Mr of 31 kDa and chitinase with a Mr of 44 kDa. Enzymes from both strains had similar characteristics and were produced during the growth phase. Both strains also produced peptaibols active against fungi in late growth and stationary phases which differed in their amino-alcohol content. The peptaibols were insecticidal when fed to larvae of Tenebrio molitor or when applied to the cuticle together with the serine protease. The results suggest that the virulence factors involved in biocontrol are the same as those for insect pathogenicity. This may affect the use of Trichoderma spp. for biocontrol as there may be effects on non-target insect species. © 2006 Elsevier Inc. All rights reserved.
Citation
Shakeri, J., & Foster, H. (2007). Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enzyme and Microbial Technology, 40(4), 961-968. https://doi.org/10.1016/j.enzmictec.2006.07.041
Journal Article Type | Article |
---|---|
Publication Date | Jan 1, 2007 |
Deposit Date | Oct 11, 2011 |
Journal | Enzyme and Microbial Technology |
Print ISSN | 0141-0229 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 40 |
Issue | 4 |
Pages | 961-968 |
DOI | https://doi.org/10.1016/j.enzmictec.2006.07.041 |
Keywords | Biological control Chitinase Entomopathogen Peptaibol Protease Trichoderma harzianum Alcohols Antibiotics Biocontrol Enzymes Insect control Pathology Entomopathogens Insect pathogens Peptaibols active Serine proteases Fungi aminoalcohol |
Publisher URL | http://dx.doi.org/10.1016/j.enzmictec.2006.07.041 |
Additional Information | Additional Information : Cited By (since 1996): 10 Export Date: 2 March 2011 Source: Scopus CODEN: EMTED Language of Original Document: English Correspondence Address: Foster, H.A.; Biomedical Sciences Research Institute, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester M5 4WT, United Kingdom; email: h.a.foster@salford.ac.uk Chemicals/CAS: chitinase, 9001-06-3; serine proteinase, 37259-58-8 References : Elad, Y., Chet, I., Katan, J., T. harzianum: a biocontrol effective against S. rolfsii and R. solani (1980) Phytopathology, 70, pp. 119-121; Elad, Y., Chet, I., Henis, Y., Biological control of Rhizoctonia solani in strawberry fields by T. harzianum (1981) Plant Soil, 60, pp. 245-254; Elad, Y., Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action (2000) Crop Prot, 19, pp. 709-714; Dubos, B., Ricard, J., Curative effect against silver leaf disease (Stereum purpurium) on peach with Trichoderma viride preparations (1974) Plant Dis Rep, 58, pp. 147-150; Witkowska, D., Maj, A., Production of lytic enzymes by Trichoderma spp. and their effect on the growth of phytopathogenic fungi (2002) Folia Microbiol, 47, pp. 279-282; Geremia, R., Goldman, G., Vila, S., Herrera-Estrella, A., Molecular characterisation of proteinase-encoding gene, prb1, related to mycoparasitism by T. harzianum (1993) Mol Microbiol, 8, pp. 603-613; De La Cruz, J., Hidalgo-Gallego, A., Llobel, A., Isolation and characterization of three chitinases from T. harzianum (1992) Eur J Biochem, 206, pp. 859-867; Ghisalberti, E., Sivasithamparam, K., Antifungal antibiotics produced by Trichoderma spp. (1991) Soil Biol Biochem, 23, pp. 1011-1020; Lida, A., Uesato, T., Okuda, M., Yashishiro, K., Fujita, T., Fungal metabolites. Part 6. Nuclear magnetic resonance study of antibiotic peptides, Trichosporin Bs, from T. polysporum (1993) J Chem Soc Perkin Trans, 1, pp. 367-373; Cherif, M., Benhamou, N., Cytochemical aspect of chitin breakdown during the parasitic action of a Trichoderma spp. on Fusarium oxysporum (1990) Phytopathology, 80, pp. 1406-1414; Flores, A., Chet, I., Herrera-Estrella, A., Improved biocontrol activity of T. harzianum by over-expression of the proteinase-encoding gene prb1 (1997) Curr Genet, 31, pp. 30-37; Clarkson, J.M., Charnley, A.K., New insights into the mechanism of fungal pathogenesis in insects (1996) Trends Microbiol, 4, pp. 197-203; St. Ledger, R.J., Charney, A.K., Cooper, R.M., Distribution of chymoelastases and trypsin-like enzymes in five species of entomopathogenic deuteromycetes (1987) Arch Biochem Biophys, 123, pp. 122-131; Jassim, H.K., Foster, H.A., Fairhurst, C.P., Biological control of Dutch elm disease: larvicidal activity of T. harzianum, T. polysporum and Scytalidium lignicola in Scolytus scolytus and S. multistriatus reared in artificial culture (1990) Ann Appl Biol, 117, pp. 187-196; Loo, Y., Skell, P., Thornberry, H., Assay of streptomycin by the paper-disc plate (1947) J Bacteriol, 50, pp. 701-709; Dennis, C., Webster, J., The antagonistic properties of the Trichoderma spp.: production of volatile antibiotics (1971) Trans Br Mycol Soc, 57, pp. 41-48; Okuda, T., Fujiwara, A., Fujiwara, M., Correlation between species of Trichoderma and production of isonitrile antibiotics (1982) Agric Biol Chem, 47, pp. 1822-1832; Betina, V., Bioautography in paper and thin layer chromatography and its scope in the antibiotic field (1973) J Chromatogr, 78, pp. 41-51; Rinderknecht, H., Gekas, M.C., Silverman, P., Haverback, B.J., A new ultrasensitive method for the determination of proteolytic activity (1968) Clin Chim Acta, 21, pp. 197-203; Bergkvist, R., The proteolytic enzymes of Aspergillus oryzae (methods for the estimation and isolation of the proteolytic enzymes) (1963) Act Chem Scand, 17, pp. 1521-1540; Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254; Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature (Lond), 224, pp. 680-685; Lineweaver, H., Burk, D., Fundamentals of enzyme kinetics (1934) J Am Chem Soc, 56, pp. 658-659; Sokal, R.R., Rohlf, F.J., (1969) Biometry, , W.H. Freeman and Company, San Francisco, USA p. 232-42; Ricard, J.L., Field observations on the biocontrol of Dutch elm disease with T. viride pellets (1983) Eur J For Pathol, 13, pp. 60-62; Murmanis, L.L., Highley, T.L., Ricard, J., Hyphal interaction of T. harzianum and T. polysporum with wood decay fungi (1987) Mat Organ, 23, pp. 271-279; Bruckner, H., Graft, H., Bokel, M., Paracelsin; characterisation of a peptaibol antibiotic from the cellulolytically active mold Trichoderma reesei (1984) Experimenta, 40, pp. 1189-1197; Fujita, T., Takaishi, Y., Matsuura, K., Takeda, Y., Yoshioka, Y., Bruckner, H., Further investigation of peptide antibiotic, hypelcin A: isolation and structures of hypelcins A-I, A-II, A-II, and A-IV (1984) Chem Pharm Bull, 32, pp. 2870-2873; Claydon, N., Allan, M., Hanson, J.R., Avent, A.G., Antifungal alkyl pyrones of T. harzianum (1987) Trans Br Mycol Soc, 88, pp. 503-513; Schirmbock, M., Lorito, M., Wang, Y., Christopher, K., Arisan, I., Scala, F., Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of T. harzianum against phytopathogenic fungi (1994) Appl Environ Microbiol, 60, pp. 4364-4370 |
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