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Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects

Shakeri, J; Foster, HA

Authors

J Shakeri

HA Foster



Abstract

Two strains of Trichoderma harzianum, 101645, an insect pathogen and 206040, used for biological control of fungal plant pathogens were investigated for the production of serine protease, chitinase and antibiotic activity in relation to entomopathogenicity. Both strains produced serine protease with a Mr of 31 kDa and chitinase with a Mr of 44 kDa. Enzymes from both strains had similar characteristics and were produced during the growth phase. Both strains also produced peptaibols active against fungi in late growth and stationary phases which differed in their amino-alcohol content. The peptaibols were insecticidal when fed to larvae of Tenebrio molitor or when applied to the cuticle together with the serine protease. The results suggest that the virulence factors involved in biocontrol are the same as those for insect pathogenicity. This may affect the use of Trichoderma spp. for biocontrol as there may be effects on non-target insect species. © 2006 Elsevier Inc. All rights reserved.

Citation

Shakeri, J., & Foster, H. (2007). Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enzyme and Microbial Technology, 40(4), 961-968. https://doi.org/10.1016/j.enzmictec.2006.07.041

Journal Article Type Article
Publication Date Jan 1, 2007
Deposit Date Oct 11, 2011
Journal Enzyme and Microbial Technology
Print ISSN 0141-0229
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 40
Issue 4
Pages 961-968
DOI https://doi.org/10.1016/j.enzmictec.2006.07.041
Keywords Biological control
Chitinase
Entomopathogen
Peptaibol
Protease
Trichoderma harzianum
Alcohols
Antibiotics
Biocontrol
Enzymes
Insect control
Pathology
Entomopathogens
Insect pathogens
Peptaibols active
Serine proteases
Fungi
aminoalcohol
Publisher URL http://dx.doi.org/10.1016/j.enzmictec.2006.07.041
Additional Information Additional Information : Cited By (since 1996): 10 Export Date: 2 March 2011 Source: Scopus CODEN: EMTED Language of Original Document: English Correspondence Address: Foster, H.A.; Biomedical Sciences Research Institute, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester M5 4WT, United Kingdom; email: h.a.foster@salford.ac.uk Chemicals/CAS: chitinase, 9001-06-3; serine proteinase, 37259-58-8
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