Characterizing tuberculosis progression in wild meerkats (Suricata suricatta) from fecal samples and clinical signs

Donadio, Josephine; Risely, Alice; Müller-Klein, Nadine; Wilhelm, Kerstin; Clutton-Brock, Tim; Manser, Marta B.; Sommer, Simone

Abstract

Tuberculosis (TB) is an increasing threat to wildlife, yet tracking its spread is challenging because infections often appear to be asymptomatic, and diagnostic tools such as blood tests can be invasive and resource intensive. Our understanding of TB biology in wildlife is therefore limited to a small number of well-studied species. Testing of fecal samples using PCR is a noninvasive method that has been used to detect Mycobacterium bovis shedding amongst badgers, yet its utility more broadly for TB monitoring in wildlife is unclear. We combined observation data of clinical signs with PCR testing of 388 fecal samples to characterize longitudinal dynamics of TB progression in 66 wild meerkats (Suricata suricatta) socially exposed to Mycobacterium suricattae between 2000 and 2018. Our specific objectives were 1) to test whether meerkat fecal samples can be used to monitor TB; 2) to characterize TB progression between three infection states (PCR-negative exposed, PCR-positive asymptomatic, and PCR positive with clinical signs); and 3) estimate individual heterogeneity in TB susceptibility, defined here as the time between TB exposure and detection, and survival after TB detection. We found that the TB detection probability once meerkats developed clinical signs was 13% (95% confidence interval 3–46%). Nevertheless, with an adapted test protocol of 10 PCR replicates per sample we detected hidden TB infections in 59% of meerkats before the onset of clinical signs. Meerkats became PCR positive approximately 14 mo after initial exposure, developed clinical signs approximately 1 yr after becoming PCR positive, and died within 5 mo of developing clinical signs. Individual variation in disease progression was high, with meerkats developing clinical signs from immediately after exposure to 3.4 yr later. Overall, our study generates novel insights into wildlife TB progression, and may help guide adapted management strategies for TB-susceptible wildlife populations.

Citation

Donadio, J., Risely, A., Müller-Klein, N., Wilhelm, K., Clutton-Brock, T., Manser, M. B., & Sommer, S. (2022). Characterizing tuberculosis progression in wild meerkats (Suricata suricatta) from fecal samples and clinical signs. Journal of Wildlife Diseases, 58(2), 309-321. https://doi.org/10.7589/JWD-D-21-00063

• Journal Article Type: Article
• Acceptance Date: Nov 3, 2021
• Publication Date: 2022
• Deposit Date: Oct 13, 2023
• Journal: Journal of Wildlife Diseases
• Print ISSN: 0090-3558
• Peer Reviewed: Peer Reviewed
• Volume: 58
• Issue: 2
• Pages: 309-321
• DOI: https://doi.org/10.7589/JWD-D-21-00063