Microbial diversity in stingless bee gut is linked to host wing size and influenced by the environment

Liu, Hongwei; Hall, Mark A.; Brettell, Laura E.; Wang, Juntao; Halcroft, Megan; Nacko, Scott; Spooner-Hart, Robert; Cook, James M.; Riegler, Markus; Singh, Brajesh K.

Abstract

Stingless bees are important social corbiculate bees, fulfilling critical pollination roles in many ecosystems. However, their gut microbiota, particularly the fungal communities associated with them, remains inadequately characterised. This knowledge gap hinders our understanding of bee gut microbiomes and their impacts on the host fitness. We collected 121 samples from two species, Tetragonula carbonaria and Austroplebeia australis across 1200 km of eastern Australia. We characterised their gut microbiomes and investigated potential correlations between bee gut microbiomes and various geographical and morphological factors. We found their core microbiomes consisted of the abundant bacterial taxa Snodgrassella, Lactobacillus and Acetobacteraceae, and the fungal taxa Didymellaceae, Monocilium mucidum and Aureobasidium pullulans, but variances of their abundances among samples were large. Furthermore, gut bacterial richness of T. carbonaria was positively correlated to host forewing length, an established correlate to body size and fitness indicator in insects relating to flight capacity. This result indicates that larger body size/longer foraging distance of bees could associate with greater microbial diversity in gut. Additionally, both host species identity and management approach significantly influenced gut microbial diversity and composition, and similarity between colonies for both species decreased as the geographic distance between them increased. We also quantified the total bacterial and fungal abundance of the samples using qPCR analyses and found that bacterial abundance was higher in T. carbonaria compared to A. australis, and fungi were either lowly abundant or below the threshold of detection for both species. Overall, our study provides novel understanding of stingless bee gut microbiomes over a large geographic span and reveals that gut fungal communities likely not play an important role in host functions due to their low abundances.

Citation

Liu, H., Hall, M. A., Brettell, L. E., Wang, J., Halcroft, M., Nacko, S., …Singh, B. K. (2023). Microbial diversity in stingless bee gut is linked to host wing size and influenced by the environment. Journal of Invertebrate Pathology, 198, 107909. https://doi.org/10.1016/j.jip.2023.107909

• Journal Article Type: Article
• Acceptance Date: Jan 1, 2023
• Publication Date: 2023-06
• Deposit Date: Jan 26, 2024
• Publicly Available Date: Jul 1, 2024
• Journal: Journal of Invertebrate Pathology
• Print ISSN: 0022-2011
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 198
• Pages: 107909
• DOI: https://doi.org/10.1016/j.jip.2023.107909
• Keywords: Ecology, Evolution, Behavior and Systematics