Dr Laura Brettell L.E.Brettell1@salford.ac.uk
University Fellow
Mosquitoes Reared in Nearby Insectaries at the Same Institution Have Significantly Divergent Microbiomes
E. Brettell, Laura; F. Hoque, Ananya; S. Joseph, Tara; Dhokiya, Vishaal; A. Hornett, Emily; L. Hughes, Grant; Heinz, Eva
Authors
Ananya F. Hoque
Tara S. Joseph
Vishaal Dhokiya
Emily A. Hornett
Grant L. Hughes
Eva Heinz
Abstract
The microbiome influences critical aspects of mosquito biology and variations in microbial composition can impact the outcomes of laboratory studies. To investigate how biotic and abiotic conditions in an insectary affect the composition of the mosquito microbiome, a single cohort of Aedes aegypti eggs was divided into three batches and transferred to three different climate‐controlled insectaries within the Liverpool School of Tropical Medicine. The bacterial microbiome composition was compared as mosquitoes developed, the microbiome of the mosquitoes' food sources was characterised, environmental conditions over time in each insectary were measured, and mosquito development and survival were recorded. While developmental success was similar across all three insectaries, differences in microbiome composition were observed between mosquitoes from each insectary. Environmental conditions and bacterial input via food sources varied between insectaries, potentially contributing to the observed differences in microbiome composition. At both adult and larval stages, specific members of the mosquito microbiome were associated with particular insectaries; the insectary with less stable and cooler conditions resulted in a slower pupation rate and higher diversity of the larval microbiome. These findings underscore that even minor inconsistencies in rearing conditions can affect the composition of the mosquito microbiome, which may influence experimental outcomes.
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Dec 3, 2024 |
| Online Publication Date | Jan 8, 2025 |
| Publication Date | Jan 1, 2025 |
| Deposit Date | Jan 15, 2025 |
| Publicly Available Date | Jan 15, 2025 |
| Journal | Environmental Microbiology |
| Print ISSN | 1462-2912 |
| Electronic ISSN | 1462-2920 |
| Publisher | Wiley |
| Peer Reviewed | Peer Reviewed |
| Volume | 27 |
| Issue | 1 |
| Article Number | e70027 |
| DOI | https://doi.org/10.1111/1462-2920.70027 |
| Keywords | environment, diversity, humidity, temperature, development, Aedes, microbiome |
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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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