MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies

Foo, Aidan; Brettell, Laura E.; Nichols, Holly L.; Medina Muñoz, Miguel; Lysne, Jessica A.; Dhokiya, Vishaal; Hoque, Ananya F.; Brackney, Doug E.; Caragata, Eric P.; Hutchinson, Michael L.; Jacobs-Lorena, Marcelo; Lampe, David J.; Martin, Edwige; Valiente Moro, Claire; Povelones, Michael; Short, Sarah M.; Steven, Blaire; Xu, Jiannong; Paustian, Timothy D.; Rondon, Michelle R.; Hughes, Grant L.; Coon, Kerri L.; Heinz, Eva

Abstract

Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species’ pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.

• Journal Article Type: Article
• Acceptance Date: Oct 11, 2024
• Online Publication Date: Nov 15, 2024
• Deposit Date: Nov 29, 2024
• Publicly Available Date: Dec 3, 2024
• Journal: PLOS Biology
• Print ISSN: 1544-9173
• Electronic ISSN: 1545-7885
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 11
• Article Number: e3002897
• DOI: https://doi.org/10.1371/journal.pbio.3002897