Skip to main content

Research Repository

Advanced Search

Prof Geoff Hide's Outputs (112)

The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system (2011)
Journal Article
Ahmed, H., MacLeod, E., Hide, G., Welburn, S., & Picozzi, K. (2011). The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system. Parasites and Vectors, 4, 68. https://doi.org/10.1186/1756-3305-4-68

Background: Diagnosis of blood borne infectious diseases relies primarily on the detection of the causative agent
in the blood sample. Molecular techniques offer sensitive and specific tools for this although considerable
difficulties exist when us... Read More about The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system.

Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle (2010)
Journal Article
Cox, A., Tosas, O., Tilley, A., Picozzi, K., Coleman, P., Hide, G., & Welburn, S. (2010). Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle. Parasites and Vectors, 3(1), 82

In East Africa, animal trypanosomiasis is caused by many tsetse transmitted protozoan parasites including Trypanosoma vivax, T. congolense and subspecies of T. brucei s.l. (T. b. brucei and zoonotic human infective T. b. rhodesiense) that may co-circ... Read More about Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle.

Molecular epidemiology of African sleeping sickness (2009)
Journal Article
Hide, G., & Tait, A. (2009). Molecular epidemiology of African sleeping sickness. Parasitology, 136(12), 1491-500. https://doi.org/10.1017/S0031182009990333

Human sleeping sickness in Africa, caused by Trypanosoma brucei spp. raises a number of questions. Despite the widespread distribution of the tsetse vectors and animal trypanosomiasis, human disease is only found in discrete foci which periodically g... Read More about Molecular epidemiology of African sleeping sickness.

Investigations by PCR analysis of neospora caninum prevalence and co-infection with toxoplasma gondii in naturally infected populations (2009)
Thesis
Hughes, J. Investigations by PCR analysis of neospora caninum prevalence and co-infection with toxoplasma gondii in naturally infected populations. (Thesis). Salford : University of Salford

Neospora caninum and Toxoplasma gondii are closely related intracellular protozoan
parasites associated with abortion in mammals. TV! caninum was identified in 1984, described
as a new species in 1988 and often previously misidentified as T. gondii... Read More about Investigations by PCR analysis of neospora caninum prevalence and co-infection with toxoplasma gondii in naturally infected populations.

Investigation into pregnancy and toxoplasma gondii infection in Libya (2009)
Thesis
Abushahma, M. Investigation into pregnancy and toxoplasma gondii infection in Libya. (Thesis). University of Salford

Toxoplasma gone/// is a parasite discovered in 1908 by Nicolle and Manceaux;
they found a protozoan in tissue of a hamster-like rodent in laboratory of the Charles Nicolle at the Pasteur Institute in Tunisia. It is an obligate intracellular
protozo... Read More about Investigation into pregnancy and toxoplasma gondii infection in Libya.

The urban house mouse (Mus domesticus) as a reservoir of infection for the human parasite Toxoplasma gondii: an unrecognised public health issue? (2008)
Journal Article
RG, M., RH, W., JM, H., G, H., NJ, F., & DJ, O. (2008). The urban house mouse (Mus domesticus) as a reservoir of infection for the human parasite Toxoplasma gondii: an unrecognised public health issue?. International Journal of Environmental Health Research, 18(3), 177-185. https://doi.org/10.1080/09603120701540856

Toxoplasma gondii is a protozoan parasite capable of infecting almost all warm-blooded animals. The cat is the definitive host and becomes infected by consuming contaminated meat or infected prey. Humans can act as intermediate hosts and in healthy i... Read More about The urban house mouse (Mus domesticus) as a reservoir of infection for the human parasite Toxoplasma gondii: an unrecognised public health issue?.

Neospora caninum : detection in wild rabbits and investigation of co-infection with Toxoplasma gondii by PCR analysis (2008)
Journal Article
Hughes, J., Thomasson, D., Craig, P., Georgin, S., Pickles, A., & Hide, G. (2008). Neospora caninum : detection in wild rabbits and investigation of co-infection with Toxoplasma gondii by PCR analysis. Experimental Parasitology, 120(3), 255-260. https://doi.org/10.1016/j.exppara.2008.07.011

Neospora caninum is an important pathogen of cattle causing significant economic loss. There is much current interest in wild animal reservoirs for this parasite. The role of the rabbit in this is currently unknown. DNA samples from the brains of wil... Read More about Neospora caninum : detection in wild rabbits and investigation of co-infection with Toxoplasma gondii by PCR analysis.

Evidence that primary infection of Charollais sheep with Toxoplasma gondii may not prevent foetal infection and abortion in subsequent lambings (2008)
Journal Article

A study carried out on a sheep farm examined whether Toxoplasma gondii foetal infection and associated abortion occur in successive lambings. We identified 29 ewes that gave birth to lambs on at least 2 successive years over our study period,2000–200... Read More about Evidence that primary infection of Charollais sheep with Toxoplasma gondii may not prevent foetal infection and abortion in subsequent lambings.

Use of molecular tools to investigate the prevalence and transmission routes of Toxoplasma in woodmice and humans (2007)
Thesis

Toxoplasma gondii is an important pathogen of humans and domestic livestock and is
a major cause of abortion. It is widely distributed amongst mammals and birds and the
epidemiology of human disease is complex due to the interactions between human... Read More about Use of molecular tools to investigate the prevalence and transmission routes of Toxoplasma in woodmice and humans.

The occurrence of the trematode Plagiorchis muris in the wood mouse Apodemus sylvaticus in North Yorkshire, UK. (2007)
Journal Article
MT, R., PS, C., G, H., S, H., A, P., & DM, S. (2007). The occurrence of the trematode Plagiorchis muris in the wood mouse Apodemus sylvaticus in North Yorkshire, UK. Journal of Helminthology, 81(1), 57 - 62. https://doi.org/10.1017/S0022149X07214105

Wood mice Apodemus sylvaticus were trapped each September over a 13-year period, from 1993 to 2005, in a wooded area adjacent to Malham Tarn, Yorkshire, UK. Plagiorchis muris was found to be the dominant intestinal trematode and occurred in every yea... Read More about The occurrence of the trematode Plagiorchis muris in the wood mouse Apodemus sylvaticus in North Yorkshire, UK..

An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom (2007)
Thesis
Morley, E. An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom. (Thesis). Salford : University of Salford

Toxoplasma gondii is an economically important parasite that can cause spontaneous
abortion in sheep and humans. The importance of the different transmission routes of
the parasite is unclear. However, three routes are recognised: (1) shedding of... Read More about An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom.

The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations (2006)
Journal Article

Neospora caninum and Toxoplasma gondii are closely related intracellular protozoan parasites associated with bovine and ovine abortion respectively. Little is known about the extent of Neospora/Toxoplasma co-infection in naturally infected population... Read More about The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations.

A PCR based assay for detection and differentiation of African trypanosome species in blood. (2005)
Journal Article
A, C., A, T., F, M., J, F., M, E., G, H., & S, W. (2005). A PCR based assay for detection and differentiation of African trypanosome species in blood. Experimental Parasitology, 111(1), 24-29. https://doi.org/10.1016/j.exppara.2005.03.014

Direct PCR analysis of trypanosome infected blood samples in the quantities required for large scale epidemiological study has always been problematic. Current methods for identifying and differentiating trypanosomes typically require several species... Read More about A PCR based assay for detection and differentiation of African trypanosome species in blood..

High levels of congenital transmission of toxoplasma gondii in longitudinal and cross-sectional studies on sheep farms provides evidence of vertical transmission in ovine hosts (2005)
Journal Article
Williams, R., Morley, E., Hughes, J., Duncanson, P., Terry, R., Smith, J., & Hide, G. (2005). High levels of congenital transmission of toxoplasma gondii in longitudinal and cross-sectional studies on sheep farms provides evidence of vertical transmission in ovine hosts. Parasitology, 130(3), 301-307. https://doi.org/10.1017/S0031182004006614

Recent research suggests that vertical transmission may play an important role in sustaining Toxoplasma gondii infection in some species. We report here that congenital transmission occurs at consistently high levels in pedigree Charollais and outbre... Read More about High levels of congenital transmission of toxoplasma gondii in longitudinal and cross-sectional studies on sheep farms provides evidence of vertical transmission in ovine hosts.

The use of molecular biological tools to investigate transmission routes and epidemiology of Toxoplasma gondii (2005)
Thesis

Toxoplasma gondii is a major cause of abortion in humans and livestock. This work focuses on three main areas. Firstly, an investigation of congenital transmission of T. gondii in sheep. Using SAG1 PCR as a detection system, high levels of congenital... Read More about The use of molecular biological tools to investigate transmission routes and epidemiology of Toxoplasma gondii.

Significant familial differences in the frequency of abortion and Toxoplasma gondii infection within a flock of Charollais sheep (2005)
Journal Article
a flock of Charollais sheep. Parasitology, 131, 181-185. https://doi.org/10.1017/S0031182005007614

A study was carried out to investigate the frequencies of abortion and congenital Toxoplasma gondii infection within 27
families (765 individuals) of a pedigree Charollais sheep flock maintained on a working farm in Worcestershire, UK, since
1992.... Read More about Significant familial differences in the frequency of abortion and Toxoplasma gondii infection within a flock of Charollais sheep.

Detection of high levels of congenital transmission of toxoplasma gondii in natural urban populations of mus domesticus (2004)
Journal Article
Marshall, P., Hughes, J., Murphy, G., Williams, R., Smith, J., Murphy, R., & Hide, G. (2004). Detection of high levels of congenital transmission of toxoplasma gondii in natural urban populations of mus domesticus. Parasitology, 128(1), 39-42. https://doi.org/10.1017/S0031182003004189

The relative importance of different transmission routes of Toxoplasma gondii has been a matter for debate. This ubiquitous parasite is generally thought to be transmitted by infective oocysts excreted by the definitive host, the cat. Ingestion of un... Read More about Detection of high levels of congenital transmission of toxoplasma gondii in natural urban populations of mus domesticus.

A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper. (2003)
Journal Article
G, H., JM, H., & R, M. (2003). A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper. BMC Ecology, 3(7), https://doi.org/10.1186/1472-6785-3-7

Background
The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum... Read More about A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper..