Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge

Rattray, NJW; Zalloum, WA; Mansell, D; Latimer, J; Schwalbe, CH; Blake, AJ; Bichenkova, EV; Freeman, S

Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

• Journal Article Type: Article
• Publication Date: May 23, 2012
• Deposit Date: Oct 24, 2016
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Publisher: Royal Society of Chemistry
• Volume: 48
• Pages: 6393-6395
• DOI: https://doi.org/10.1039/C2CC32521G
• Publisher URL: http://dx.doi.org/10.1039/C2CC32521G
• Related Public URLs: http://pubs.rsc.org/en/Journals/JournalIssues/CC#!recentarticles&adv