Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti

Logan, Rhiannon A. E.; Quek, Shannon; Muthoni, Joseph N.; von Eicken, Anneliese; Brettell, Laura E.; Anderson, Enyia R.; Villena, Marcus E. N.; Hegde, Shivanand; Patterson, Grace T.; Heinz, Eva; Hughes, Grant L.; Patterson, Edward I.

Abstract

Cell fusing agent virus (CFAV) is an insect-specific flavivirus (ISF) found in Aedes aegypti mosquitoes. ISFs have demonstrated the ability to modulate the infection or transmission of arboviruses such as dengue, West Nile, and Zika viruses. It is thought that vertical transmission is the main route for ISF maintenance in nature. This has been observed with CFAV, but there is evidence of horizontal and venereal transmission in other ISFs. Understanding the route of transmission can inform strategies to spread ISFs to vector populations as a method of controlling pathogenic arboviruses. We crossed individually reared male and female mosquitoes from both a naturally occurring CFAV-positive Ae. aegypti colony and its negative counterpart to provide information on maternal, paternal, and horizontal transmission. RT-PCR was used to detect CFAV in individual female pupal exuviae and was 89% sensitive, but only 42% in male pupal exuviae. This is a possible way to screen individuals for infection without destroying the adults. Female-to-male horizontal transmission was not observed during this study. However, there was a 31% transmission rate from mating pairs of CFAV-positive males to negative female mosquitoes. Maternal vertical transmission was observed with a filial infection rate of 93%. The rate of paternal transmission was 85% when the female remained negative, 61% when the female acquired CFAV horizontally, and 76% overall. Maternal and paternal transmission of CFAV could allow the introduction of this virus into wild Ae. aegypti populations through male or female mosquito releases, and thus provides a potential strategy for ISF-derived arbovirus control.

Citation

Logan, R. A. E., Quek, S., Muthoni, J. N., von Eicken, A., Brettell, L. E., Anderson, E. R., …Patterson, E. I. (2022). Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti. Applied and Environmental Microbiology, 88(18), https://doi.org/10.1128/aem.01062-22

• Journal Article Type: Article
• Acceptance Date: Aug 5, 2022
• Publication Date: Sep 22, 2022
• Deposit Date: Jan 26, 2024
• Publicly Available Date: Jan 30, 2024
• Journal: Applied and Environmental Microbiology
• Print ISSN: 0099-2240
• Electronic ISSN: 1098-5336
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 88
• Issue: 18
• DOI: https://doi.org/10.1128/aem.01062-22
• Keywords: Ecology; Applied Microbiology and Biotechnology; Food Science; Biotechnology