Dr Pika Miklavc's Outputs (23)

Myosin-1C augments secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane. (2024)
Journal Article
El-Mansi, S., Mitchell, T. P., Mobyen, G., McKinnon, T. A. J., Miklavc, P., Frick, M., …McKinnon, T. A. (in press). Myosin-1C augments secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane. #Journal not on list, https://doi.org/10.1182/bloodadvances.2024012590

Blood endothelial cells control the hemostatic and inflammatory response by secreting von Willebrand factor (VWF) and P-selectin from storage organelles called Weibel-Palade bodies (WPB). Actin-associated motor proteins regulate this secretory pathwa... Read More about Myosin-1C augments secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane..

Wedge resection versus lobectomy in T1 lung cancer patients: a propensity matched analysis (2023)
Journal Article
Moon, R. J., Taylor, R., Miklavc, P., Mehdi, S. B., Grant, S. W., & Bittar, M. N. (in press). Wedge resection versus lobectomy in T1 lung cancer patients: a propensity matched analysis. Journal of Cardiothoracic Surgery, 18(1), 252. https://doi.org/10.1186/s13019-023-02303-4

Objectives: Performing wedge resection rather than lobectomy for primary lung cancer remains controversial. Recent studies demonstrate no survival advantage for non-anatomical resection compared to lobectomy in patients with early-stage lung cancer.... Read More about Wedge resection versus lobectomy in T1 lung cancer patients: a propensity matched analysis.

Actin and myosin in non-neuronal exocytosis (2020)
Journal Article
Miklavc, P., & Frick, M. (2020). Actin and myosin in non-neuronal exocytosis. Cells, 9(6), e1455. https://doi.org/10.3390/cells9061455

Cellular secretion depends on exocytosis of secretory vesicles and discharge of vesicle contents. Actin and myosin are essential for pre-fusion and post-fusion stages of exocytosis. Secretory vesicles depend on actin for transport to and attachment a... Read More about Actin and myosin in non-neuronal exocytosis.

Interaction of microtubules and actin during the post-fusion phase of exocytosis (2019)
Journal Article
Muller, M., Schempp, R., Lutz, A., Felder, T., Felder, E., & Miklavc, P. (2019). Interaction of microtubules and actin during the post-fusion phase of exocytosis. Scientific reports, 9, 11973. https://doi.org/10.1038/s41598-019-47741-0

Exocytosis is the intracellular trafficking step where a secretory vesicle fuses with the plasma membrane to release vesicle content. Actin and microtubules both play a role in exocytosis; however, their interplay is not understood. Here we study the... Read More about Interaction of microtubules and actin during the post-fusion phase of exocytosis.

Silver nanoparticles impair retinoic acid-inducible gene I mediated mitochondrial anti-viral immunity by blocking the autophagic flux in lung epithelial cells (2018)
Journal Article
Villeret, B., Dieu, A., Straube, M., Solhonne, B., Miklavc, P., Hamadi, S., …Garcia-Verdugo, I. (2018). Silver nanoparticles impair retinoic acid-inducible gene I mediated mitochondrial anti-viral immunity by blocking the autophagic flux in lung epithelial cells. ACS nano, 12(2), 1188-1202. https://doi.org/10.1021/acsnano.7b06934

Silver nanoparticles (AgNPs) are microbicidal agents which could be potentially used as alternative to antivirals to treat human infectious diseases, especially Influenza virus infection where antivirals have generally proven unsuccessful. However, c... Read More about Silver nanoparticles impair retinoic acid-inducible gene I mediated mitochondrial anti-viral immunity by blocking the autophagic flux in lung epithelial cells.

A small key unlocks a heavy door : the essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies (2016)
Journal Article
Hobi, N., Giolai, M., Olmeda, B., Miklavc, P., Felder, E., Walther, P., …Haller, T. (2016). A small key unlocks a heavy door : the essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies. BBA - Molecular Cell Research, 1863(8), 2124-2134. https://doi.org/10.1016/j.bbamcr.2016.04.028

The molecular basis involving adsorption of pulmonary surfactant at the respiratory air–liquid interface and the specific roles of the surfactant proteins SP-B and SP-C in this process have not been completely resolved. The reasons might be found in... Read More about A small key unlocks a heavy door : the essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies.

The role of myosin 1c and myosin 1b in surfactant exocytosis (2016)
Journal Article
Kittelberger, N., Breunig, M., Martin, R., Knölker, H., & Miklavc, P. (2016). The role of myosin 1c and myosin 1b in surfactant exocytosis. Journal of Cell Science, 129(8), 1685-1696. https://doi.org/10.1242/jcs.181313

Actin and actin-associated proteins have a pivotal effect on regulated exocytosis in secretory cells and influence pre-fusion as well as post-fusion stages of exocytosis. Actin polymerization on secretory granules during the post-fusion phase (format... Read More about The role of myosin 1c and myosin 1b in surfactant exocytosis.

A new role for an old drug: Ambroxol triggers lysosomal exocytosis via pH-dependent Ca2+ release from acidic Ca2+ stores (2015)
Journal Article
Fois, G., Hobi, N., Felder, E., Ziegler, A., Miklavc, P., Walther, P., …Dietl, P. (2015). A new role for an old drug: Ambroxol triggers lysosomal exocytosis via pH-dependent Ca2+ release from acidic Ca2+ stores. Cell Calcium, 58(6), 628-637. https://doi.org/10.1016/j.ceca.2015.10.002

Ambroxol (Ax) is a frequently prescribed drug used to facilitate mucociliary clearance, but its mode of action is yet poorly understood. Here we show by X-ray spectroscopy that Ax accumulates in lamellar bodies (LBs), the surfactant storing, secretor... Read More about A new role for an old drug: Ambroxol triggers lysosomal exocytosis via pH-dependent Ca2+ release from acidic Ca2+ stores.

Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles (2015)
Journal Article
Miklavc, P., Ehinger, K., Sultan, A., Felder, T., Paul, P., Gottschalk, K., & Frick, M. (2015). Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles. Journal of Cell Science, 128(6), 1193-1203. https://doi.org/10.1242/jcs.165571

In many secretory cells actin and myosin are specifically recruited to the surface of secretory granules following their fusion with the plasma membrane. Actomyosin-dependent compression of fused granules is essential to promote active extrusion of c... Read More about Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles.

Surfactant secretion in LRRK2 knock-out rats : changes in lamellar body morphology and rate of exocytosis (2014)
Journal Article
Miklavc, P., Ehinger, K., Thompson, K., Hobi, N., Shimshek, D., & Frick, M. (2014). Surfactant secretion in LRRK2 knock-out rats : changes in lamellar body morphology and rate of exocytosis. PLoS ONE, 9(1), e84926. https://doi.org/10.1371/journal.pone.0084926

Leucine-rich repeat kinase 2 (LRRK2) is known to play a role in the pathogenesis of various diseases including Parkinson disease, morbus Crohn, leprosy and cancer. LRRK2 is suggested to be involved in a number of cell biological processes such as ves... Read More about Surfactant secretion in LRRK2 knock-out rats : changes in lamellar body morphology and rate of exocytosis.

A new role for P2X4 receptors as modulators of lung surfactant secretion (2013)
Journal Article
Miklavc, P., Thompson, K., & Frick, M. (2013). A new role for P2X4 receptors as modulators of lung surfactant secretion. Frontiers in Cellular Neuroscience, 7, https://doi.org/10.3389/fncel.2013.00171

In recent years, P2X receptors have attracted increasing attention as regulators of exocytosis and cellular secretion. In various cell types, P2X receptors have been found to stimulate vesicle exocytosis directly via Ca2+ influx and elevation of the... Read More about A new role for P2X4 receptors as modulators of lung surfactant secretion.

Actin coating and compression of fused secretory vesicles are essential for surfactant secretion - a role for Rho, formins and myosin II (2012)
Journal Article
Miklavc, P., Hecht, E., Hobi, N., Wittekindt, O., Dietl, P., Kranz, C., & Frick, M. (2012). Actin coating and compression of fused secretory vesicles are essential for surfactant secretion - a role for Rho, formins and myosin II. Journal of Cell Science, 125(11), 2765-2774. https://doi.org/10.1242/jcs.105262

Secretion of vesicular contents by exocytosis is a fundamental cellular process. Increasing evidence suggests that post-fusion events play an important role in determining the composition and quantity of the secretory output. In particular, regulatio... Read More about Actin coating and compression of fused secretory vesicles are essential for surfactant secretion - a role for Rho, formins and myosin II.

An ultra fast detection method reveals strain-induced Ca2+ entry via TRPV2 in alveolar type II cells (2011)
Journal Article
Fois, G., Wittekindt, O., Zheng, X., Felder, E. T., Miklavc, P., Frick, M., …Felder, E. (2012). An ultra fast detection method reveals strain-induced Ca2+ entry via TRPV2 in alveolar type II cells. Biomechanics and Modeling in Mechanobiology, 11(7), 959-971. https://doi.org/10.1007/s10237-011-0365-4

A commonly used technique to investigate strain-induced responses of adherent cells is culturing them on an elastic membrane and globally stretching the membrane. However, it is virtually impossible to acquire microscopic images immediately after the... Read More about An ultra fast detection method reveals strain-induced Ca2+ entry via TRPV2 in alveolar type II cells.

Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins: Streptolysin O activates SOC entry (2011)
Journal Article
Usmani, S. M., von Einem, J., Frick, M., Miklavc, P., Mayenburg, M., Husmann, M., …Wittekindt, O. H. (2012). Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins: Streptolysin O activates SOC entry. Cellular Microbiology, 14(3), 299-315. https://doi.org/10.1111/j.1462-5822.2011.01724.x

Streptolysin O (SLO) is a cholesterol-dependent cytolysin (CDC) from Streptococcus pyogenes. SLO induces diverse types of Ca2+ signalling in host cells which play a key role in membrane repair and cell fate determination. The mechanisms behind SLO-in... Read More about Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins: Streptolysin O activates SOC entry.

Vesicular calcium channels as regulators of the exocytotic post-fusion phase (2011)
Journal Article
Miklavc, P., & Frick, M. (2011). Vesicular calcium channels as regulators of the exocytotic post-fusion phase. Communicative and Integrative Biology, 4(6), 796-798. https://doi.org/10.4161/cib.17935

Regulated secretion is a fundamental cellular process in many different types of eukaryotic cells with Ca2+-triggered exocytosis taking centre stage. Elevations of the cytoplasmic Ca2+ concentration ([Ca2+]c) regulate multiple steps from vesicle fusi... Read More about Vesicular calcium channels as regulators of the exocytotic post-fusion phase.

Chemotopy of Amino Acids on the Olfactory Bulb Predicts Olfactory Discrimination Capabilities of Zebrafish Danio rerio (2011)
Journal Article
Miklavc, P., & Valentincic, T. (2012). Chemotopy of Amino Acids on the Olfactory Bulb Predicts Olfactory Discrimination Capabilities of Zebrafish Danio rerio. Chemical Senses, 37(1), 65-75. https://doi.org/10.1093/chemse/bjr066

Amino acids reliably evoke strong responses in fish olfactory system. The molecular olfactory receptors (ORs) are located in the membrane of cilia and microvilli of the olfactory receptor neurons (ORNs). Axons of ORNs converge on specific olfactory b... Read More about Chemotopy of Amino Acids on the Olfactory Bulb Predicts Olfactory Discrimination Capabilities of Zebrafish Danio rerio.

Olfactory discrimination of complex mixtures of amino acids by the black bullhead Ameiurus melas (2011)
Journal Article
Valentincic, T., Miklavc, P., Kralj, S., & Zgonik, V. (2011). Olfactory discrimination of complex mixtures of amino acids by the black bullhead Ameiurus melas. Journal of Fish Biology, 79(1), 33-52. https://doi.org/10.1111/j.1095-8649.2011.02976.x

On the basis of previous findings of behavioural discrimination of amino acids and on the knowledge of electrophysiology of the catfish (genera Ictalurus and Ameiurus) olfactory organs, behavioural experiments that investigated olfactory discriminati... Read More about Olfactory discrimination of complex mixtures of amino acids by the black bullhead Ameiurus melas.

Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes (2011)
Journal Article
Miklavc, P., Mair, N., Wittekindt, O., Haller, T., Dietl, P., Felder, E., …Frick, M. (2011). Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes. Proceedings of the National Academy of Sciences of the United States of America, 108(35), 14503-14508. https://doi.org/10.1073/pnas.1101039108

Ca2+ is considered a key element in multiple steps during regulated exocytosis. During the postfusion phase, an elevated cytoplasmic Ca2+ concentration ([Ca2+])c leads to fusion pore dilation. In neurons and neuroendocrine cells, this results from ac... Read More about Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes.

Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes (2010)
Journal Article
Miklavc, P., Frick, M., Wittekindt, O., Haller, T., & Dietl, P. (2010). Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes. PLoS ONE, 5(6), e10982. https://doi.org/10.1371/journal.pone.0010982

Background

Ca2+ is essential for vesicle fusion with the plasma membrane in virtually all types of regulated exocytoses. However, in contrast to the well-known effects of a high cytoplasmic Ca2+ concentration ([Ca2+]c) in the prefusion phase, the... Read More about Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes.

Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes (2009)
Journal Article
Miklavc, P., Albrecht, S., Wittekindt, O., Schullian, P., Haller, T., & Dietl, P. (2009). Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes. Biochemical Journal, 424(1), 7-14. https://doi.org/10.1042/BJ20091094

Exocytosis proceeds through prefusion stages such as hemifusion, but hemifusion is still an elusive intermediate of unknown duration. Using darkfield and fluorescence microscopy in ATII (alveolar type II) cells containing large secretory vesicles (LB... Read More about Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes.