Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells (2011)
Journal Article
and distribution in live mammalian cells. European Journal of Cancer, 48(12), 1896-1903. https://doi.org/10.1016/j.ejca.2011.11.025

To investigate within live mammalian cells the uptake and disposition of combretastatins, fluorescence lifetime imaging was used with two-photon excitation (2PE). Combretastatin A4 (CA4) and analogues are potential anticancer drugs due to their abili... Read More about Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells.

Energy dependence of gold nanoparticle radiosensitization in plasmid DNA (2011)
Journal Article
McMahon, S., Hyland, W., Brun, E., Butterworth, K., Coulter, J., Douki, T., …Sicard-Roselli, C. (2011). Energy dependence of gold nanoparticle radiosensitization in plasmid DNA. Journal of Physical Chemistry C, 115(41), 20160-20167. https://doi.org/10.1021/jp206854s

Gold nanopa rticles (GNPs) are of considerableinterest for use as a radiosensitizer, because of their biocompat-ibility and their ability to increase dose deposited because oftheir high mass energy absorption coefficient. Their sensitizingproperties ha... Read More about Energy dependence of gold nanoparticle radiosensitization in plasmid DNA.

Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs (2011)
Journal Article
Elkord, E., Sharma, S., Burt, D., & Hawkins, R. (2011). Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs. Clinical Immunology, 140(3), 218-222. https://doi.org/10.1016/j.clim.2011.04.014

Conversion of conventional T cells into T regulatory cells (Tregs) has been proposed as a potential mechanism for Treg expansion in cancer. However, this evidence is supported by in vitro or mouse model studies with no data from in vivo or human stud... Read More about Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs.

Intracellular mapping with SERS-encoded gold nanostars (2011)
Journal Article
Rodríguez-Lorenzo, L., Krpetic, Z., Barbosa, S., Alvarez-Puebla, R., Liz-Marzán, L., Prior, I., & Brust, M. (2011). Intracellular mapping with SERS-encoded gold nanostars. Integrative Biology, 3(9), 922-926. https://doi.org/10.1039/C1IB00029B

Here we report on the design, synthesis and application of small, highly bright, star-shaped SERS encoded single nanoparticles with the ability of providing an optical signal upon excitation with near infrared light. These particles are colloidally s... Read More about Intracellular mapping with SERS-encoded gold nanostars.

Acrylate-facilitated cellular uptake of gold nanoparticles (2011)
Journal Article
Krpetic, Z., Nativo, P., Prior, I., & Brust, M. (2011). Acrylate-facilitated cellular uptake of gold nanoparticles. Small, 7(14), 1982-1986. https://doi.org/10.1002/smll.201100462

Uptake of PEGylated gold nanoparticles by HeLa cells in the presence of acrylate is described. Nanoparticles that are usually not taken up readily cross the plasma membrane into the cytosol in the presence of acrylate. In addition, a method is descri... Read More about Acrylate-facilitated cellular uptake of gold nanoparticles.

Immunotherapy: what is its role in prostate cancer? (2011)
Journal Article
Elkord, E. (2011). Immunotherapy: what is its role in prostate cancer?

Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles (2011)
Journal Article
Krpeticc, Z., Saleemi, S., Prior, I., Sée, V., Qureshi, R., & Brust, M. (2011). Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles. ACS nano, 5(6), 5195-5201. https://doi.org/10.1021/nn201369k

This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. The TAT peptide... Read More about Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles.

Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure (2011)
Journal Article
Briston, S., Caldwell, J., Horn, M., Clarke, J., Richards, M., Greensmith, D., …Trafford, A. (2011). Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure. Journal of Physiology, 589(Pt 6), 1367-82. https://doi.org/10.1113/jphysiol.2010.203984

Reduced inotropic responsiveness is characteristic of heart failure (HF). This study determined the cellular Ca2+ homeostatic and molecular mechanisms causing the blunted β-adrenergic (β-AR) response in HF.We induced HF by tachypacing in sheep; intra... Read More about Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure.

Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells (2011)
Journal Article
Elkord, E., & Burt, D. (2011). Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells. Scandinavian Journal of Immunology, 74(4), 419-422. https://doi.org/10.1111/j.1365-3083.2011.02588.x

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical t... Read More about Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells.

Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells (2011)
Journal Article
Khan, S., Burt, D., Ralph, C., Hawkins, R., & Elkord, E. (2011). Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells. Clinical Immunology, 138(1), 85-96. https://doi.org/10.1016/j.clim.2010.09.011

Cytotoxic T Lymphocyte Antigen 4 (CTLA4) blockade has shown antitumor activity against common cancers. However, the exact mechanism of immune mediation by anti-CTLA4 remains to be elucidated. Further understanding of how CTLA4 blockade with tremelimu... Read More about Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells.

Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status (2011)
Journal Article
Sawan, S., Burt, D., Stern, P., Holland, C., & Elkord, E. (2011). Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status. Immunological Investigations, 40(1), 62-75. https://doi.org/10.3109/08820139.2010.513022

Regulatory T cells (Treg) are a sub-population of T cells that suppress self-reactivity and are implicated in immune tolerance towards malignant cells. Circulating Treg cells are increased in several cancers. In endometrial cancer Treg cells have bee... Read More about Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status.

Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats (2011)
Journal Article
Thomasson, D., Wright, E., Hughes, J., Dodd, N., Cox, A., Boyce, K., …Hide, G. (2011). Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats. Parasitology, 138(09), 1117-1123. https://doi.org/10.1017/S0031182011000904

The protozoan parasite Toxoplasma gondii is prevalent worldwide and can infect a remarkably wide range of hosts despite felids being the only definitive host. As cats play a major role in transmission to secondary mammalian hosts, the interaction b... Read More about Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats.

The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa (2011)
Journal Article
Fothergill, J., Mowat, E., Walshaw, M., Ledson, M., James, C., & Winstanley, C. (2011). The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 55(1), 426-428. https://doi.org/10.1128/AAC.01257-10

Phage production in response to antibiotics varied among four isolates of a Pseudomonas aeruginosa cystic fibrosis (CF) epidemic strain. Whereas ciprofloxacin induced higher levels of phage production, other CF-relevant antibiotics led to reduced p... Read More about The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.