Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production (2021)
Journal Article
Monahan, T., Baker, J. A., Krabben, P., Davies, E. T., Jenkinson, E. R., Goodhead, I. B., …Shepherd, M. (2021). Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production. Microbial Biotechnology, 15(5), 1574-1585. https://doi.org/10.1111/1751-7915.13990

SummaryThe development and advent of mutagenesis tools for solventogenic clostridial species in recent years has allowed for the increased refinement of industrially relevant strains. In this study we have utilised CLEAVE™, a CRISPR/Cas genome editin... Read More about Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production.

Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation (2021)
Journal Article
Arrigo, R., Blume, R., Streibel, V., Genovese, C., Roldan, A., Schuster, M., …Centi, G. (2022). Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation. ACS catalysis, 12(1), 411-430. https://doi.org/10.1021/acscatal.1c04296

Surface-sensitive ambient pressure X-ray photoelectron spectroscopy and near edge X-ray absorption fine structure spectroscopy combined with an electrocatalytic reactivity study, multilength scale electron microscopy and theo-retical modelling provid... Read More about Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation.

Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population (2021)
Journal Article
Cyprian, F., Suleman, M., Abdelhafez, I., Doudin, A., Masud Danjuma, I., Mir, F., …Girardi, G. (2021). Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population. Frontiers in Immunology, 12, 707159. https://doi.org/10.3389/fimmu.2021.707159

Coronavirus disease-2019 (COVID-19) was declared as a pandemic by WHO in March 2020. SARS-CoV-2 causes a wide range of illness from asymptomatic to life-threatening. There is an essential need to identify biomarkers to predict disease severity and mo... Read More about Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population.

Facile synthesis and biological evaluation of chrysin derivatives (2021)
Journal Article
Omonga, N., Zia, Z., Ghanbour, H., Ragazzon-Smith, A., Foster, H., Hadfield, J., & Ragazzon, P. (2021). Facile synthesis and biological evaluation of chrysin derivatives. Journal of Chemical Research, 45(11-12), 1083-1092. https://doi.org/10.1177/17475198211057467

In this paper, novel synthetic methods, including microwave O-alkylation, were used to produce several chrysin derivatives. These compounds were purified, characterised and tested on different cell lines and bacterial strains. From this family, 7-(2,... Read More about Facile synthesis and biological evaluation of chrysin derivatives.

Selected derivatives of erythromycin B- in silico and anti-malarial studies (2021)
Journal Article
Bhadra, P., Magwaza, R., Nirmalan, N., Freeman, S., Barber, J., & Arsic, B. (2021). Selected derivatives of erythromycin B- in silico and anti-malarial studies. Materials, 14(22), e6980. https://doi.org/10.3390/ma14226980

Erythromycin A is an established anti-bacterial agent against Gram-positive bacteria, but it is unstable to acid. This led to an evaluation of erythromycin B and its derivatives because these have improved acid stability. These compounds were investi... Read More about Selected derivatives of erythromycin B- in silico and anti-malarial studies.

High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats (2021)
Journal Article
Wang, Z., Yu, S., Gao, J., Zhang, P., Hide, G., Yamamoto, M., …Lun, Z. (2021). High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats. iScience, 24(11), 103280. https://doi.org/10.1016/j.isci.2021.103280

Nitric oxide (NO) is an important immune molecule which acts against extra- and intracellular pathogens in most hosts. However, after knockout of inducible nitric oxide synthase (iNOS-/-) in Sprague Dawley (SD) rats, these iNOS-/- rats were found to... Read More about High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats.

High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy (2021)
Journal Article
Fiorillo, M., Ozsvari, B., Sotgia, F., & Lisanti, M. (2021). High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy. Frontiers in Oncology, 11, 740720. https://doi.org/10.3389/fonc.2021.740720

Recently, we presented evidence that high mitochondrial ATP production is a new therapeutic target for cancer treatment. Using ATP as a biomarker, we isolated the “metabolically fittest” cancer cells from the total cell population. Importantly, ATP-h... Read More about High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy.

Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam (2021)
Thesis
Bui, H. Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam. (Thesis). University of Salford

Autism spectrum disorder (ASD) is a neurodevelopmental disorder with a prevalence of approximately 1% children worldwide. ASD is characterized by deficits in social communication and interaction and the presence of restricted interests and repetitive... Read More about Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam.

SnoRNAs and miRNAs networks underlying COVID-19 disease severity (2021)
Journal Article
Parray, A., Mir, F., Doudin, A., Iskandarani, A., Danjuma, I., Kuni, R., …Cyprian, F. (2021). SnoRNAs and miRNAs networks underlying COVID-19 disease severity. Vaccines, 9(10), e1056. https://doi.org/10.3390/vaccines9101056

There is a lack of predictive markers for early and rapid identification of disease progression in COVID-19 patients. Our study aims at identifying microRNAs (miRNAs)/small nucleolar RNAs (snoRNAs) as potential biomarkers of COVID-19 severity. Using... Read More about SnoRNAs and miRNAs networks underlying COVID-19 disease severity.

A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME) (2021)
Journal Article
Mishra, S., Manzoor, K., Zafar, M., & Podmore, I. (2021). A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME). Free Radical Research, 55(5), 569-578. https://doi.org/10.1080/10715762.2021.1980563

In this study, we have utilized a novel strategy based upon the use of dimethyl sulfoxide (DMSO) and gas chromatography-mass spectrometry (GC-MS)for the detectionand identification ofspin-trapped free radicals. Hydroxymethyl (.CH2OH) radicals, genera... Read More about A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME).

SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line (2021)
Journal Article
Di Pisa, F., Pesenti, E., Bono, M., Mazzarello, A., Bernardi, C., Lisanti, M., …Ghiotto, F. (2021). SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line. BMC Molecular and Cell Biology, 22(1), 41. https://doi.org/10.1186/s12860-021-00379-1

Background: The human SH3 domain Binding Glutamic acid Rich Like 3 (SH3BGRL3) gene is highly conserved in phylogeny and widely expressed in human tissues. However, its function is largely undetermined. The protein was found to be overexpressed in sev... Read More about SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line.

Novel diarylamides and diarylureas with N-substitution dependent activity against medulloblastoma (2021)
Journal Article
Lawson, C., Ahmed Alta, T., Moschou, G., Skamnaki, V., Solovou, T., Topham, C., …Snape, T. (2021). Novel diarylamides and diarylureas with N-substitution dependent activity against medulloblastoma. European Journal of Medicinal Chemistry, 225, 113751. https://doi.org/10.1016/j.ejmech.2021.113751

Medulloblastoma - highly aggressive and heterogeneous tumours of the cerebellum - account for 15-20% of all childhood brain tumours, and are the most common high-grade childhood embryonal tumour of the central nervous system. Herein, potent in vitro... Read More about Novel diarylamides and diarylureas with N-substitution dependent activity against medulloblastoma.

Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one (2021)
Journal Article
Mohammed, S., & Ferry, N. (2021). Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one. International Journal of Molecular Sciences, 22(15), e8284. https://doi.org/10.3390/ijms22158284

Sialic acid (Sia) is considered as one of the most important biomolecules of life since its derivatives and terminal orientations on cell membranes and macromolecules play a major role in many biological and pathological processes. To date, there is... Read More about Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one.

MitoTracker Deep Red (MTDR) is a metabolic inhibitor for targeting mitochondria and eradicating cancer stem cells (CSCs), with anti-tumor and anti-metastatic activity in vivo (2021)
Journal Article
Sargiacomo, C., Stonehouse, S., Moftakhar, Z., Sotgia, F., & Lisanti, M. (2021). MitoTracker Deep Red (MTDR) is a metabolic inhibitor for targeting mitochondria and eradicating cancer stem cells (CSCs), with anti-tumor and anti-metastatic activity in vivo. Frontiers in Oncology, 11, 678343. https://doi.org/10.3389/fonc.2021.678343

MitoTracker Deep Red (MTDR) is a relatively non-toxic, carbocyanine-based, far-red, fluorescent probe that is routinely used to chemically mark and visualize mitochondria in living cells. Previously, we used MTDR at low nano-molar concentrations to... Read More about MitoTracker Deep Red (MTDR) is a metabolic inhibitor for targeting mitochondria and eradicating cancer stem cells (CSCs), with anti-tumor and anti-metastatic activity in vivo.

Integrated whole transcriptome and small RNA analysis revealed multiple regulatory networks in colorectal cancer (2021)
Journal Article
Shaath, H., Toor, S., Nada, M., Elkord, E., & Alajez, N. (2021). Integrated whole transcriptome and small RNA analysis revealed multiple regulatory networks in colorectal cancer. Scientific reports, 11(1), 14456. https://doi.org/10.1038/s41598-021-93531-y

Colorectal cancer (CRC) remains a global disease burden and a leading cause of cancer related deaths worldwide. The identification of aberrantly expressed messenger RNA (mRNA), long non-coding RNA (lncRNA), and microRNA (miRNA), and the resulting mol... Read More about Integrated whole transcriptome and small RNA analysis revealed multiple regulatory networks in colorectal cancer.

SPARC 2021 - Against all odds : Salford postgraduate annual research conference book of abstracts (2021)
Book
Thomas, A., Ibrahim, A., Abraham, A., Halilu, A., Adeosun, A., Court, A., …Whitehead, H. (2021). H. Bury, S. Gowda, T. Ireland, L. Mitchell, E. Oginni, T. Poppelreuter, …D. Whitnall (Eds.), SPARC 2021 - Against all odds : Salford postgraduate annual research conference book of abstracts. Salford, United Kingdom: University of Salford

Welcome to the Book of Abstracts for the 2021 SPARC conference. Our conference is called “Against All Odds” as we celebrate the achievements of our PGRs and their supervisors, who have continued to excel despite the most challenging circumstances. Fo... Read More about SPARC 2021 - Against all odds : Salford postgraduate annual research conference book of abstracts.

The chemotherapeutic potential of Boswellia carterii oleoresins (2021)
Thesis
Jones, M. The chemotherapeutic potential of Boswellia carterii oleoresins. (Thesis). University of Salford

Cancer is a leading cause of death worldwide. Though effective treatments are available and reduce mortality, issues such as debilitating side-effects and drug resistance necessitate a need to develop alternatives. Natural products are a key and dive... Read More about The chemotherapeutic potential of Boswellia carterii oleoresins.

Monitoring dynamics of defects and single Fe atoms in N-functionalized few-layer graphene by in situ temperature programmed scanning transmission electron microscopy (2021)
Journal Article
Arrigo, R., Sasaki, T., Callison, J., Gianolio, D., & Schuster, M. (2022). Monitoring dynamics of defects and single Fe atoms in N-functionalized few-layer graphene by in situ temperature programmed scanning transmission electron microscopy. Journal of Energy Chemistry, 64, 520-530. https://doi.org/10.1016/j.jechem.2021.05.005

In this study, we aim to contribute an understanding of the pathway of formation of Fe species during top-down synthesis of dispersed Fe on N-functionalized few layer graphene. We use X-ray absorption spectroscopy to determine the electronic structur... Read More about Monitoring dynamics of defects and single Fe atoms in N-functionalized few-layer graphene by in situ temperature programmed scanning transmission electron microscopy.

The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease (2021)
Thesis
Alawfi, B. (2021). The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease. (Thesis). University of Salford

Toxoplasma gondii is a zoonotic parasite that commonly causes infections in a variety of warm-blooded animals including humans. This parasite causes a disease referred to as toxoplasmosis. T. gondii infections are known to have a significant impact o... Read More about The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease.

Exploring potential inhibitors of the HGF-Met signalling pathway as a prospective treatment for paediatric medulloblastoma (2021)
Thesis
Hoyles, N. Exploring potential inhibitors of the HGF-Met signalling pathway as a prospective treatment for paediatric medulloblastoma. (Dissertation). University of Salford

Understanding the function of the HGF-Met signalling axis could present opportunities for new therapeutic treatments for cancer patients. The brain tumour Medulloblastoma is the leading cause of cancer-related death in children and infants meaning re... Read More about Exploring potential inhibitors of the HGF-Met signalling pathway as a prospective treatment for paediatric medulloblastoma.