Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes (2018)
Journal Article
Molitor, A., James, C., Fanning, S., Pages, J., & Davin-Regli, A. (2018). Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes. Journal of Medical Microbiology, 67(2), 148-159. https://doi.org/10.1099/jmm.0.000667

Purpose: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the eme... Read More about Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes.

Campylobacter jejuni transcriptome changes during loss of culturability in water (2017)
Journal Article
Bronowski, C., Mustafa, K., Goodhead, I., James, C., Nelson, C., Lucaci, A., …Winstanley, C. (2017). Campylobacter jejuni transcriptome changes during loss of culturability in water. PLoS ONE, 12(11), e0188936. https://doi.org/10.1371/journal.pone.0188936

Background: Water serves as a potential reservoir for Campylobacter, the leading cause of bacterial gastroenteritis in humans. However, little is understood about the mechanisms underlying variations in survival characteristics between different st... Read More about Campylobacter jejuni transcriptome changes during loss of culturability in water.

Evolutionary diversification of Pseudomonas aeruginosa in an artificial sputum model (2017)
Journal Article
Davies, E., James, C., Brockhurst, M., & Winstanley, C. (2017). Evolutionary diversification of Pseudomonas aeruginosa in an artificial sputum model. BMC Microbiology, 17(3), https://doi.org/10.1186/s12866-016-0916-z

Background During chronic lung infections of cystic fibrosis patients Pseudomonas aeruginosa populations undergo extensive evolutionary diversification. However, the selective drivers of this evolutionary process are poorly understood. To test the e... Read More about Evolutionary diversification of Pseudomonas aeruginosa in an artificial sputum model.

Recent advances in studies of polymicrobial interactions in oral biofilms (2013)
Journal Article
James, C. (2013). Recent advances in studies of polymicrobial interactions in oral biofilms. Current Oral Health Reports, 1(1), 59-69. https://doi.org/10.1007/s40496-013-0006-3

The oral cavity supports a complex and finely balanced consortium of microbial species, many of which cooperate within structured biofilms. These communities develop through multitudinous synergistic and antagonistic interspecies relationships. Chang... Read More about Recent advances in studies of polymicrobial interactions in oral biofilms.

Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung (2012)
Journal Article
Kirchner, S., Fothergill, J., Wright, E., James, C., Mowat, E., & Winstanley, C. (2012). Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung. Journal of Visualized Experiments, 64, 1-8. https://doi.org/10.3791/3857

There is growing concern about the relevance of in vitro antimicrobial susceptibility tests when applied to isolates of P. aeruginosa from cystic fibrosis (CF) patients. Existing methods rely on single or a few isolates grown aerobically and plankto... Read More about Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung.

Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa (2012)
Journal Article
James, C., Fothergill, J., Kalwij, H., Hall, A., Cottell, J., Brockhurst, M., & Winstanley, C. (2012). Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa. BMC Microbiology, 12(216), 1-10. https://doi.org/10.1186/1471-2180-12-216

Background: Pseudomonas aeruginosa is the most common bacterial pathogen infecting the lungs of patients with cystic fibrosis (CF). The Liverpool Epidemic Strain (LES) is transmissible, capable of superseding other P. aeruginosa populations and is... Read More about Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa.

The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa (2011)
Journal Article
Fothergill, J., Mowat, E., Walshaw, M., Ledson, M., James, C., & Winstanley, C. (2011). The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 55(1), 426-428. https://doi.org/10.1128/AAC.01257-10

Phage production in response to antibiotics varied among four isolates of a Pseudomonas aeruginosa cystic fibrosis (CF) epidemic strain. Whereas ciprofloxacin induced higher levels of phage production, other CF-relevant antibiotics led to reduced p... Read More about The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.

How beta-lactam antibiotics enter bacteria: a dialogue with the porins (2009)
Journal Article
James, C., Mahendran, K., Molitor, A., Bolla, J., Bessonov, A., Winterhalter, M., & Pages, J. (2009). How beta-lactam antibiotics enter bacteria: a dialogue with the porins. PLoS ONE, 4(5), e5453. https://doi.org/10.1371/journal.pone.0005453

BACKGROUND:Multi-drug resistant (MDR) infections have become a major concern in hospitals worldwide. This study investigates membrane translocation, which is the first step required for drug action on internal bacterial targets. beta-lactams, a major... Read More about How beta-lactam antibiotics enter bacteria: a dialogue with the porins.

Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. (2008)
Journal Article
Davin-Regli, A., Bolla, J., James, C., Lavigne, J., Chevalier, J., Garnotel, E., …Pagès, J. (2008). Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. Current Drug Targets, 9(9), 750-759. https://doi.org/10.2174/138945008785747824

In Enterobacteriaceae, membrane permeability is a key in the level of susceptibility to antibiotics. Modification of the bacterial envelope by decreasing the porin production or increasing the expression of efflux pump systems has been reported. Thes... Read More about Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens..

The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria (2008)
Journal Article
Pages, J., James, C., & Winterhalter, M. (2008). The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria. Nature Reviews Microbiology, 6, 893-903. https://doi.org/10.1038/nrmicro1994

Gram-negative bacteria are responsible for a large proportion of antibiotic resistant bacterial diseases. These bacteria have a complex cell envelope that comprises an outer membrane and an inner membrane that delimit the periplasm. The outer membran... Read More about The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria.

Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria (2007)
Journal Article
Smith, D. L., James, C., Sergeant, M., Yaxian, Y., Saunders, J. R., McCarthy, A. J., & Allison, H. (2007). Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria. Journal of Bacteriology, 189, 7223-7233. https://doi.org/10.1128/JB.00824-07

Infection of Escherichia coli by Shiga toxin-encoding bacteriophages (Stx phages) was the pivotal event in the evolution of the deadly Shiga toxin-encoding E. coli (STEC), of which serotype O157:H7 is the most notorious. The number of different bacte... Read More about Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria.

An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins (2007)
Journal Article
Dupont, M., James, C., Chevalier, J., & Pages, J. (2007). An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins. Antimicrobial Agents and Chemotherapy, 51(9), 3190-3198. https://doi.org/10.1128/AAC.01481-06

Bacterial adaptation to external stresses and toxic compounds is a key step in the emergence of multidrug-resistant strains that are a serious threat to human health. Although some of the proteins and regulators involved in antibiotic resistance mech... Read More about An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins.

HIV protease inhibitors are substrates for OATP1A2, OATP1B1 and OATP1B3 and lopinavir plasma concentrations are influenced by SLCO1B1 polymorphisms
Journal Article
Hartkoorn, R., Kwan, W., Shallcross, V., Chaikan, A., Liptrott, N., Egan, D., …Owen, A. HIV protease inhibitors are substrates for OATP1A2, OATP1B1 and OATP1B3 and lopinavir plasma concentrations are influenced by SLCO1B1 polymorphisms. Pharmacogenetics and Genomics, 20(2), 112-120. https://doi.org/10.1097/FPC.0b013e328335b02d

OBJECTIVE: OATP1B1 and OATP1B3 are major hepatic drug transporters whilst OATP1A2 is mainly located in the brain but is also located in liver and several other organs. These transporters affect the distribution and clearance of many endobiotics and x... Read More about HIV protease inhibitors are substrates for OATP1A2, OATP1B1 and OATP1B3 and lopinavir plasma concentrations are influenced by SLCO1B1 polymorphisms.

Novel therapeutic strategies to counter pseudomonas aeruginosa infections
Journal Article
Fothergill, J., Winstanley, C., & James, C. Novel therapeutic strategies to counter pseudomonas aeruginosa infections. Expert Review of Anti-infective Therapy, 10(2), 219-235. https://doi.org/10.1586/eri.11.168

Pseudomonas aeruginosa is a highly successful opportunistic pathogen that displays intrinsic multidrug resistance and has a tremendous capacity to acquire further resistance mechanisms. During chronic infection, the bacterium can form a protective... Read More about Novel therapeutic strategies to counter pseudomonas aeruginosa infections.