Circular dichroism to determine binding mode and affinity of ligand-DNA interactions (2007)
Journal Article
Garbett, N., Ragazzon, P., & Chaires, J. (2007). Circular dichroism to determine binding mode and affinity of ligand-DNA interactions. Nature Protocols, 2(12), 3166-3172

Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of in... Read More about Circular dichroism to determine binding mode and affinity of ligand-DNA interactions.

Use of competition dialysis in the discovery of G-quadruplex selective ligands (2007)
Journal Article
Ragazzon, P., & Chaires, J. (2007). Use of competition dialysis in the discovery of G-quadruplex selective ligands. Methods, 43(4), 313-323

G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand oreintations, to bimolecular dimeric structures, and finally to tetramolecular parallel-stranded... Read More about Use of competition dialysis in the discovery of G-quadruplex selective ligands.

Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya (2007)
Thesis
Gusbi, M. Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya. (Thesis). University of Salford

Two studies were performed to detect aetiology of acute diarrhoea in hospitalized children aged under five years in Tripoli, Libya. In the first study, two hundred and thirty-six stool samples were obtained. Of these, 118 were from patients admitted... Read More about Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya.

Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. (2007)
Journal Article
Perez-Campo, F., Spencer, H., Elder, R., Stern, P., & Ward, C. (2007). Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. Experimental Cell Research, 313(16), 3604-15. https://doi.org/10.1016/j.yexcr.2007.07.021

The use of gene mutation/knock-out strategies in mouse embryonic stem (ES) cells has revolutionized the study of gene function in ES cells and embryonic development. However, the construction of vectors for homologous recombination strategies require... Read More about Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter..

Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria (2007)
Journal Article
Smith, D. L., James, C., Sergeant, M., Yaxian, Y., Saunders, J. R., McCarthy, A. J., & Allison, H. (2007). Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria. Journal of Bacteriology, 189, 7223-7233. https://doi.org/10.1128/JB.00824-07

Infection of Escherichia coli by Shiga toxin-encoding bacteriophages (Stx phages) was the pivotal event in the evolution of the deadly Shiga toxin-encoding E. coli (STEC), of which serotype O157:H7 is the most notorious. The number of different bacte... Read More about Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria.

An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins (2007)
Journal Article
Dupont, M., James, C., Chevalier, J., & Pages, J. (2007). An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins. Antimicrobial Agents and Chemotherapy, 51(9), 3190-3198. https://doi.org/10.1128/AAC.01481-06

Bacterial adaptation to external stresses and toxic compounds is a key step in the emergence of multidrug-resistant strains that are a serious threat to human health. Although some of the proteins and regulators involved in antibiotic resistance mech... Read More about An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins.

Gold nanoparticles capped by peptides (2007)
Journal Article
Porta, F., Speranza, G., Krpetic, Z., Dal Santo, V., Francescato, P., & Scarì, G. (2007). Gold nanoparticles capped by peptides. Materials Science and Engineering: B, 140(3), 187-194. https://doi.org/10.1016/j.mseb.2007.03.019

Two dipeptides (GK and GC) and two 15-aminoacid peptides (GK15 and GC15) were used as capping agents in the preparation of monolayer-protected gold nanoparticles (MPCs). They were characterized by TEM microscopy, UV–vis, NMR and IR spectroscopy.

Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. (2007)
Journal Article
Crisostomo, A., Moreno, R., Navaratnam, S., Wilkinson, J., & Bisby, R. (2007). Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. Free Radical Research, 41(6), 730-737. https://doi.org/10.1080/10715760701324075

Three potential routes to generation of reactive oxygen species from a tocopherolquinone have been identified. The quinone of the water-soluble vitamin E analogue Trolox C (Trol-Q) is reduced by hydrated electron and isopropanol a hydroxyalkyl radica... Read More about Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues..

Unravelling the moulting degradome: new opportunities for chemotherapy? (2007)
Journal Article
Craig, H., Isaac, R., & Brooks, D. (2007). Unravelling the moulting degradome: new opportunities for chemotherapy?. Trends in Parasitology, 23(6), 248-253. https://doi.org/10.1016/j.pt.2007.04.003

Replacement of the nematode cuticle with a newly synthesized cuticle (a process known as moulting) occurs four times during larval development. Therefore, the key components of this essential developmental process represent attractive targets for new... Read More about Unravelling the moulting degradome: new opportunities for chemotherapy?.

Competition dialysis: a method for the study of structural selective nucleic acid binding (2007)
Journal Article
Ragazzon, P., Garbett, N., & Chaires, J. (2007). Competition dialysis: a method for the study of structural selective nucleic acid binding. Methods, 42(2), 173-182. https://doi.org/10.1016/j.ymeth.2006.09.010

Competition dialysis is a powerful new tool for the discovery of ligands that bind to nucleic acids with structural- or sequence-selectivity. The method is based on firm thermodynamic principles and is simple to implement. In the competition dialysis... Read More about Competition dialysis: a method for the study of structural selective nucleic acid binding.

Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold (2007)
Journal Article
Alcaro, S., Artese, A., Iley, J., Maccari, R., Missailidis, S., Ortuso, F., …Vigorita, M. (2007). Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold. Bioorganic and Medicinal Chemistry Letters, 1(17), 2509-2514

A series of fluorenone-carboxamide compounds was analyzed with regard to DNA binding properties by UV spectroscopy and competition dialysis methods. The morpholino derivative 10 provided interesting results in terms of affinity and specificity toward... Read More about Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold.

Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus (2007)
Journal Article
to the laminated layer of echinococcus granulosus. Parasitology Research, 101(3), 647-652. https://doi.org/10.1007/s00436-007-0534-7

The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate... Read More about Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus.

Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection (2007)
Journal Article
Morgan, C., & Mitchell, A. (2007). Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection. Biosensors and Bioelectronics, 22(8), 1769-1775. https://doi.org/10.1016/j.bios.2006.08.024

Biological assays to detect binding interactions are often conducted using fluorescence resonance energy transfer (FRET) but this has several disadvantages that markedly reduce the dynamic range of measurements. The very short range of FRET interacti... Read More about Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection.

Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system (2007)
Journal Article
Botchway, S., Crisostomo, A., Parker, A., & Bisby, R. (2007). Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system. Archives of Biochemistry and Biophysics, 464(2), 314-321. https://doi.org/10.1016/j.abb.2007.04.026

Solutions of tryptophan and N-hydroxypyridine-2-thione (mercaptopyridine-N-oxide, MPNO) were irradiated at 335 nm. Formation of 5-hydroxytryptophan was inferred from increased fluorescence at 334 nm on excitation at 315 nm, conditions chosen for sele... Read More about Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system.

Frequency of regulatory T cells in renal cell carcinoma patients and investigation of correlation with survival (2007)
Journal Article
Griffiths, R., Elkord, E., Gilham, D., Ramani, V., Clarke, N., Stern, P., & Hawkins, R. (2007). Frequency of regulatory T cells in renal cell carcinoma patients and investigation of correlation with survival. Cancer Immunology, Immunotherapy, 56(11), 1743-1753. https://doi.org/10.1007/s00262-007-0318-z

Regulatory T cells are important in maintaining immune homeostasis, mediating peripheral tolerance and preventing autoimmunity. Increased frequencies of CD4(+)CD25(high )T regulatory (T(Reg)) cells have been documented in the peripheral blood of pati... Read More about Frequency of regulatory T cells in renal cell carcinoma patients and investigation of correlation with survival.

Immunology and immunotherapy approaches for prostate cancer (2007)
Journal Article
Elkord, E. (2007). Immunology and immunotherapy approaches for prostate cancer. Prostate Cancer and Prostatic Diseases, 10(3), 224-236. https://doi.org/10.1038/sj.pcan.4500964

Several mechanisms that impair the immune response to promote tumour progression are reported. These mechanisms aim to reduce the ability of antigen-presenting cells to present antigen and activate naïve T cells to support an active immune response o... Read More about Immunology and immunotherapy approaches for prostate cancer.

An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom (2007)
Thesis
Morley, E. An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom. (Thesis). Salford : University of Salford

Toxoplasma gondii is an economically important parasite that can cause spontaneous abortion in sheep and humans. The importance of the different transmission routes of the parasite is unclear. However, three routes are recognised: (1) shedding of... Read More about An investigation into the congenital transmission of Toxoplasma gondii within ovine populations managed on working sheep farms in the United Kingdom.

A study of the cytotoxic effects of methionine depletion in human brain tumour cell lines (2007)
Thesis
Najim, N. A study of the cytotoxic effects of methionine depletion in human brain tumour cell lines. (Thesis). Salford : University of Salford

The aim of this project was to investigate the importance of methionine depletion as a cause of cytotoxicity for paediatric CNS tumour cell lines, and also to investigate the in vitro cellular biochemical responses, as measured by changes in cellul... Read More about A study of the cytotoxic effects of methionine depletion in human brain tumour cell lines.

Diblock polyampholytes grafted onto spherical particles : effect of stiffness, charge density, and grafting density (2007)
Journal Article
Akinchina, A., & Linse, P. (2007). Diblock polyampholytes grafted onto spherical particles : effect of stiffness, charge density, and grafting density. Langmuir, 23(3), 1465-1472. https://doi.org/10.1021/la062481r

The structure of spherical brushes formed by symmetric diblock polyampholytes end-grafted onto small spherical particles in aqueous solution is examined within the framework of the so-called primitive model using Monte Carlo simulations. The proper... Read More about Diblock polyampholytes grafted onto spherical particles : effect of stiffness, charge density, and grafting density.