Evaluation of small extracellular vesicular microRNAs as biomarkers for exposure of nicotine and E-liquids on human lung cells (2022)
Thesis
for exposure of nicotine and E-liquids on human lung cells. (Thesis). University of Salford

Nicotine intake and E-Cigarette or vaping product use-associated lung injury (EVALI) can cause pulmonary disease with damaging health effects. In this study, we explored miRNAs from small extracellular vesicles (sEVs) for nicotine and E-liquid-associ... Read More about Evaluation of small extracellular vesicular microRNAs as biomarkers for exposure of nicotine and E-liquids on human lung cells.

Targeting c-met in sonic hedgehog medulloblastomas (2022)
Thesis
Morlando, S. Targeting c-met in sonic hedgehog medulloblastomas. (Thesis). University of Salford

Medulloblastoma (MB), a cerebellar tumour primarily diagnosed in children, is the most common malignant brain tumour consisting of four molecular subgroups: Wingless (WNT), Sonic Hedgehog (SHH), Group 3 and Group 4. Although the 70-75% of MB patients... Read More about Targeting c-met in sonic hedgehog medulloblastomas.

Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater) (2022)
Journal Article
Madubuike, H., & Ferry, N. (2022). Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater). Molecules, 27(9), 2999. https://doi.org/10.3390/molecules27092999

Acetyl xylan esterases (AXEs) are enzymes capable of hydrolysing the acetyl bonds in acetylated xylan, allowing for enhanced activity of backbone-depolymerizing enzymes. Bioprospecting novel AXE is essential in designing enzyme cocktails with desired... Read More about Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater).

Elimination of Off-Target Effect by Chemical Modification of 5'-End of Small Interfering RNA (2022)
Journal Article
Shiohama, Y., Fujita, R., Sonokawa, M., Hisano, M., Kotake, Y., Krstic- Demonacos, M., …Fujii, M. (2022). Elimination of Off-Target Effect by Chemical Modification of 5'-End of Small Interfering RNA. Nucleic Acid Therapeutics, 32(5), 438-447. https://doi.org/10.1089/nat.2021.0068

In this study, the efficiency of RNA interference of small interfering RNAs (siRNAs) bearing 5′-O-methyl-2′-deoxythymidine (X) and 5′-amino-2′, 5′-dideoxythymidine (Z) at the 5′-end of the sense strand and the antisense strand of siRNA was investigat... Read More about Elimination of Off-Target Effect by Chemical Modification of 5'-End of Small Interfering RNA.

Inhibitory Immune Checkpoint Receptors and Ligands as Prognostic Biomarkers in COVID-19 Patients. (2022)
Journal Article
Al-Mterin, M. A., Alsalman, A., & Elkord, E. (2022). Inhibitory Immune Checkpoint Receptors and Ligands as Prognostic Biomarkers in COVID-19 Patients. Frontiers in Immunology, 13, 870283. https://doi.org/10.3389/fimmu.2022.870283

Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2. During T-cell activation, the immune system uses different checkpoint pathways to maintain co-inhibitory and co-stimulatory signals. In COVID-19, expression of immune checkpoints (ICs) is o... Read More about Inhibitory Immune Checkpoint Receptors and Ligands as Prognostic Biomarkers in COVID-19 Patients..

Transplantation of Gut Microbiota From High-Fat-Diet-Tolerant Cynomolgus Monkeys Alleviates Hyperlipidemia and Hepatic Steatosis in Rats. (2022)
Journal Article
Gao, J., Rao, J., Wei, Z., Xia, S., Huang, L., Tang, M., …Chen, J. (2022). Transplantation of Gut Microbiota From High-Fat-Diet-Tolerant Cynomolgus Monkeys Alleviates Hyperlipidemia and Hepatic Steatosis in Rats. Frontiers in Microbiology, 13, 876043. https://doi.org/10.3389/fmicb.2022.876043

Emerging evidence has been reported to support the involvement of the gut microbiota in the host's blood lipid and hyperlipidemia (HLP). However, there remains unexplained variation in the host's blood lipid phenotype. Herein a nonhuman primate HLP m... Read More about Transplantation of Gut Microbiota From High-Fat-Diet-Tolerant Cynomolgus Monkeys Alleviates Hyperlipidemia and Hepatic Steatosis in Rats..

Comparison of 3 randomized clinical trials of frontline therapies for malignant pleural mesothelioma (2022)
Journal Article
Meirson, T., Pentimalli, F., Cerza, F., Baglio, G., Gray, S., Correale, P., …Mutti, L. (2022). Comparison of 3 randomized clinical trials of frontline therapies for malignant pleural mesothelioma. Jama Network Open, 5(3), e221490. https://doi.org/10.1001/jamanetworkopen.2022.1490

Importance: Some recently proposed frontline therapies for malignant pleural mesothelioma (MPM) are very costly, yet their impact on quality of life and overall survival of these patients remains arguable. Given the high social toll of this aggressiv... Read More about Comparison of 3 randomized clinical trials of frontline therapies for malignant pleural mesothelioma.

Apropos of everything: navigating life with genre (2022)
Thesis
Flynn, C. (2022). Apropos of everything: navigating life with genre. (Thesis). University of Salford

Apropos of Everything: Navigating Life with Genre (AoE) is a hybrid critical/creative portfolio which proposes a new theory of Genre, Creativity and Consciousness, named The Social Consciousness Theory (SoCo). By reclaiming and recalibrating the conc... Read More about Apropos of everything: navigating life with genre.

Protein disulfide isomerase A1‑associated pathways in the development of stratified breast cancer therapies (2022)
Journal Article
Bakker, E., Fujii, M., Krstic‑Demonacos, M., Demonacos, C., & Alhammad, R. (2022). Protein disulfide isomerase A1‑associated pathways in the development of stratified breast cancer therapies. International Journal of Oncology, 60(2), 16. https://doi.org/10.3892/ijo.2022.5306

The oxidoreductase protein disulfide isomerase A1 (PDIA1) functions as a cofactor for many transcription factors including estrogen receptor α (ERα), nuclear factor (NF)‑κB, nuclear factor erythroid 2‑like 2 (NRF2) and regulates the protein stability... Read More about Protein disulfide isomerase A1‑associated pathways in the development of stratified breast cancer therapies.

Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production (2021)
Journal Article
Monahan, T., Baker, J. A., Krabben, P., Davies, E. T., Jenkinson, E. R., Goodhead, I. B., …Shepherd, M. (2021). Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production. Microbial Biotechnology, 15(5), 1574-1585. https://doi.org/10.1111/1751-7915.13990

SummaryThe development and advent of mutagenesis tools for solventogenic clostridial species in recent years has allowed for the increased refinement of industrially relevant strains. In this study we have utilised CLEAVE™, a CRISPR/Cas genome editin... Read More about Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production.

Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation (2021)
Journal Article
Arrigo, R., Blume, R., Streibel, V., Genovese, C., Roldan, A., Schuster, M., …Centi, G. (2022). Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation. ACS catalysis, 12(1), 411-430. https://doi.org/10.1021/acscatal.1c04296

Surface-sensitive ambient pressure X-ray photoelectron spectroscopy and near edge X-ray absorption fine structure spectroscopy combined with an electrocatalytic reactivity study, multilength scale electron microscopy and theo-retical modelling provid... Read More about Dynamics at polarized, carbon dioxide-iron oxyhydroxide interfaces unveil the origin of multicarbon product formation.

Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population (2021)
Journal Article
Cyprian, F., Suleman, M., Abdelhafez, I., Doudin, A., Masud Danjuma, I., Mir, F., …Girardi, G. (2021). Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population. Frontiers in Immunology, 12, 707159. https://doi.org/10.3389/fimmu.2021.707159

Coronavirus disease-2019 (COVID-19) was declared as a pandemic by WHO in March 2020. SARS-CoV-2 causes a wide range of illness from asymptomatic to life-threatening. There is an essential need to identify biomarkers to predict disease severity and mo... Read More about Complement C5a and clinical markers as predictors of COVID-19 disease severity and mortality in a multi-ethnic population.

Facile synthesis and biological evaluation of chrysin derivatives (2021)
Journal Article
Omonga, N., Zia, Z., Ghanbour, H., Ragazzon-Smith, A., Foster, H., Hadfield, J., & Ragazzon, P. (2021). Facile synthesis and biological evaluation of chrysin derivatives. Journal of Chemical Research, 45(11-12), 1083-1092. https://doi.org/10.1177/17475198211057467

In this paper, novel synthetic methods, including microwave O-alkylation, were used to produce several chrysin derivatives. These compounds were purified, characterised and tested on different cell lines and bacterial strains. From this family, 7-(2,... Read More about Facile synthesis and biological evaluation of chrysin derivatives.

Selected derivatives of erythromycin B- in silico and anti-malarial studies (2021)
Journal Article
Bhadra, P., Magwaza, R., Nirmalan, N., Freeman, S., Barber, J., & Arsic, B. (2021). Selected derivatives of erythromycin B- in silico and anti-malarial studies. Materials, 14(22), e6980. https://doi.org/10.3390/ma14226980

Erythromycin A is an established anti-bacterial agent against Gram-positive bacteria, but it is unstable to acid. This led to an evaluation of erythromycin B and its derivatives because these have improved acid stability. These compounds were investi... Read More about Selected derivatives of erythromycin B- in silico and anti-malarial studies.

High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats (2021)
Journal Article
Wang, Z., Yu, S., Gao, J., Zhang, P., Hide, G., Yamamoto, M., …Lun, Z. (2021). High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats. iScience, 24(11), 103280. https://doi.org/10.1016/j.isci.2021.103280

Nitric oxide (NO) is an important immune molecule which acts against extra- and intracellular pathogens in most hosts. However, after knockout of inducible nitric oxide synthase (iNOS-/-) in Sprague Dawley (SD) rats, these iNOS-/- rats were found to... Read More about High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats.

High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy (2021)
Journal Article
Fiorillo, M., Ozsvari, B., Sotgia, F., & Lisanti, M. (2021). High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy. Frontiers in Oncology, 11, 740720. https://doi.org/10.3389/fonc.2021.740720

Recently, we presented evidence that high mitochondrial ATP production is a new therapeutic target for cancer treatment. Using ATP as a biomarker, we isolated the “metabolically fittest” cancer cells from the total cell population. Importantly, ATP-h... Read More about High ATP production fuels cancer drug resistance and metastasis : implications for mitochondrial ATP depletion therapy.

Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam (2021)
Thesis
Bui, H. Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam. (Thesis). University of Salford

Autism spectrum disorder (ASD) is a neurodevelopmental disorder with a prevalence of approximately 1% children worldwide. ASD is characterized by deficits in social communication and interaction and the presence of restricted interests and repetitive... Read More about Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam.

SnoRNAs and miRNAs networks underlying COVID-19 disease severity (2021)
Journal Article
Parray, A., Mir, F., Doudin, A., Iskandarani, A., Danjuma, I., Kuni, R., …Cyprian, F. (2021). SnoRNAs and miRNAs networks underlying COVID-19 disease severity. Vaccines, 9(10), e1056. https://doi.org/10.3390/vaccines9101056

There is a lack of predictive markers for early and rapid identification of disease progression in COVID-19 patients. Our study aims at identifying microRNAs (miRNAs)/small nucleolar RNAs (snoRNAs) as potential biomarkers of COVID-19 severity. Using... Read More about SnoRNAs and miRNAs networks underlying COVID-19 disease severity.

A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME) (2021)
Journal Article
Mishra, S., Manzoor, K., Zafar, M., & Podmore, I. (2021). A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME). Free Radical Research, 55(5), 569-578. https://doi.org/10.1080/10715762.2021.1980563

In this study, we have utilized a novel strategy based upon the use of dimethyl sulfoxide (DMSO) and gas chromatography-mass spectrometry (GC-MS)for the detectionand identification ofspin-trapped free radicals. Hydroxymethyl (.CH2OH) radicals, genera... Read More about A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME).

SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line (2021)
Journal Article
Di Pisa, F., Pesenti, E., Bono, M., Mazzarello, A., Bernardi, C., Lisanti, M., …Ghiotto, F. (2021). SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line. BMC Molecular and Cell Biology, 22(1), 41. https://doi.org/10.1186/s12860-021-00379-1

Background: The human SH3 domain Binding Glutamic acid Rich Like 3 (SH3BGRL3) gene is highly conserved in phylogeny and widely expressed in human tissues. However, its function is largely undetermined. The protein was found to be overexpressed in sev... Read More about SH3BGRL3 binds to myosin 1c in a calcium dependent manner and modulates migration in the MDA-MB-231 cell line.