Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations (2011)
Journal Article
Nirmalan, N., Hughes, C., Peng, J., McKenna, T., Langridge, J., Cairns, D., …Banks, R. (2011). Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations. Journal of Proteome Research, 10(2), 896-906. https://doi.org/10.1021/pr100812d

Annotated formalin-fixed, paraffin-embedded
(FFPE) tissue archives constitute a valuable resource for
retrospective biomarker discovery. However, proteomic
exploration of archival tissue is impeded by extensive
formalin-induced covalent cross-lin... Read More about Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations.

Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting (2009)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2009). Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting. The Journal of pathology, 217(4), 497-506. https://doi.org/10.1002/path.2504

The development of efficient formaldehyde cross-link reversal strategies will make the vast
diagnostic tissue archives of pathology departments amenable to prospective and retrospective
translational research, particularly in biomarker-driven prote... Read More about Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting.

Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges (2008)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2008). Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges. Molecular BioSystems, 4(7), 712-720. https://doi.org/10.1039/b800098k

The significant potential of tissue-based proteomic biomarker studies can be restricted
by difficulties in accessing samples in optimal fresh-frozen form. While archival formalinfixed
tissue collections with attached clinical and outcome data repre... Read More about Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges.