All Outputs (10)

Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production (2021)
Journal Article
Monahan, T., Baker, J. A., Krabben, P., Davies, E. T., Jenkinson, E. R., Goodhead, I. B., …Shepherd, M. (2021). Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production. Microbial Biotechnology, 15(5), 1574-1585. https://doi.org/10.1111/1751-7915.13990

SummaryThe development and advent of mutagenesis tools for solventogenic clostridial species in recent years has allowed for the increased refinement of industrially relevant strains. In this study we have utilised CLEAVE™, a CRISPR/Cas genome editin... Read More about Deletion of glyceraldehyde-3-phosphate dehydrogenase (gapN) in Clostridium saccharoperbutylacetonicum N1-4(HMT) using CLEAVE™ increases the ATP pool and accelerates solvent production.

Comparative analysis of gene prediction tools for viral genome annotation (2021)
Journal Article
Gonzalez-Tortuero, E., Krishnamurthi, R., Allison, H., Goodhead, I., & James, C. Comparative analysis of gene prediction tools for viral genome annotation. https://doi.org/10.1101/2021.12.11.472104. Manuscript submitted for publication

The number of newly available viral genomes and metagenomes has increased exponentially since the development of high throughput sequencing platforms and genome analysis tools. Bioinformatic annotation pipelines are largely based on open reading fram... Read More about Comparative analysis of gene prediction tools for viral genome annotation.

Characterising the bacterial microbiota of Phlebotomus and Glossina insects : vectors of parasitic diseases (2021)
Thesis
Pescod, P. (2021). Characterising the bacterial microbiota of Phlebotomus and Glossina insects : vectors of parasitic diseases. (Thesis). University of Salford

As the technology used to examine bacteria using culture-independent methods has advanced, so too has our knowledge of the bacterial microbiome of insect vectors and its effect on the pathogens they carry. Trypanosomiasis and leishmaniasis are neglec... Read More about Characterising the bacterial microbiota of Phlebotomus and Glossina insects : vectors of parasitic diseases.

Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam (2021)
Thesis
Bui, H. Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam. (Thesis). University of Salford

Autism spectrum disorder (ASD) is a neurodevelopmental disorder with a prevalence of approximately 1% children worldwide. ASD is characterized by deficits in social communication and interaction and the presence of restricted interests and repetitive... Read More about Investigation of de-novo copy number variants in patients with Autism Spectrum Disorder in Vietnam.

Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome (2021)
Journal Article
Harrison, X., McDevitt, A., Dunn, J., Grifftihs, S., Benvenuto, C., Birtles, R., …Antwis, R. (2021). Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome. Proceedings of the Royal Society B: Biological Sciences, 288(1957), 20210552. https://doi.org/10.1098/rspb.2021.0552

Interactions between hosts and their resident microbial communities are a fundamental component of fitness for both agents. Though recent research has highlighted the importance of interactions between animals and their bacterial communities, compara... Read More about Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome.

Antibiotic resistance profiles and population structure of disease-associated Staphylococcus aureus infecting patients in Fort Portal Regional Referral Hospital, Western Uganda (2021)
Journal Article
Ackers-Johnson, G., Kibombo, D., Kusiima, B., Nsubuga, M., Kigozi, E., Kajumbula, H., …James, C. (2021). Antibiotic resistance profiles and population structure of disease-associated Staphylococcus aureus infecting patients in Fort Portal Regional Referral Hospital, Western Uganda. Microbiology, 167(5), 001000. https://doi.org/10.1099/mic.0.001000

Tackling antimicrobial resistance (AMR) is particularly challenging in low-resource settings such as Fort Portal Regional Referral Hospital (FPRRH) in Western Uganda. Specific knowledge of local AMR epidemiology is required to inform evidence-based i... Read More about Antibiotic resistance profiles and population structure of disease-associated Staphylococcus aureus infecting patients in Fort Portal Regional Referral Hospital, Western Uganda.

Nitric oxide (NO) elicits aminoglycoside tolerance in Escherichia coli but antibiotic resistance gene carriage and NO sensitivity have not co-evolved (2021)
Journal Article
Ribiero, C., Rahman, L., Holmes, L., Woody, A., Webster, C., Monaghan, T., …Shepherd, M. (2021). Nitric oxide (NO) elicits aminoglycoside tolerance in Escherichia coli but antibiotic resistance gene carriage and NO sensitivity have not co-evolved. Archives of Microbiology, 203(5), 2541-2550. https://doi.org/10.1007/s00203-021-02245-2

The spread of multidrug-resistance in Gram-negative bacterial pathogens presents a major clinical challenge, and new approaches are required to combat these organisms. Nitric oxide (NO) is a well-known antimicrobial that is produced by the immune sys... Read More about Nitric oxide (NO) elicits aminoglycoside tolerance in Escherichia coli but antibiotic resistance gene carriage and NO sensitivity have not co-evolved.

Proinflammatory cytokines as mediators of phenotypic change in bacteria implicated in ventilator-associated pneumonia (2021)
Thesis
Tompsett, A. Proinflammatory cytokines as mediators of phenotypic change in bacteria implicated in ventilator-associated pneumonia. (Thesis). University of Salford

Ventilator-associated pneumonia (VAP) is a common hospital-acquired infection in intensive care. VAP is associated with increased hospital stay, healthcare costs, and high mortality. VAP is difficult to diagnose and manage appropriately, leading to w... Read More about Proinflammatory cytokines as mediators of phenotypic change in bacteria implicated in ventilator-associated pneumonia.

Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome (2021)
Other
Harrison, X., McDevitt, A., Dunn, J., Griffiths, S., Benvenuto, C., Birtles, R., …Antwis, R. (2021). Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome

Interactions between hosts and their resident microbial communities are a fundamental component of fitness for both agents. Though recent research has highlighted the importance of interactions between animals and their bacterial communities, compara... Read More about Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome.

Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding (2021)
Journal Article
Naaum, A., Cusa, M., Singh, M., Bleicher, Z., Elliott, C., Goodhead, I., …Sanchez, J. (2021). Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding. Food Research International, 141, 110035. https://doi.org/10.1016/j.foodres.2020.110035

Seafood represents up to 20% of animal protein consumption in global food consumption and is a critical dietary and income resource for the world’s population. Currently, over 30% of marine fish stocks are harvested at unsustainable levels, and the i... Read More about Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding.