Tunicamycin induced endoplasmatic reticulum changes in endothelial cells investigated in vitro by confocal Raman imaging (2019)
Journal Article
endothelial cells investigated in vitro by confocal Raman imaging. Analyst, 144(22), 6561-6569. https://doi.org/10.1039/C9AN01456J

This paper describes how tunicamycin (Tu), the most widely used pharmacological agent for inducing endoplasmic reticulum (ER) stress, interacts with endothelial cells. Our results show that tunicamycin enters the cells and accumulates within the ER a... Read More about Tunicamycin induced endoplasmatic reticulum changes in endothelial cells investigated in vitro by confocal Raman imaging.

Interaction of microtubules and actin during the post-fusion phase of exocytosis (2019)
Journal Article
Muller, M., Schempp, R., Lutz, A., Felder, T., Felder, E., & Miklavc, P. (2019). Interaction of microtubules and actin during the post-fusion phase of exocytosis. Scientific reports, 9, 11973. https://doi.org/10.1038/s41598-019-47741-0

Exocytosis is the intracellular trafficking step where a secretory vesicle fuses with the plasma membrane to release vesicle content. Actin and microtubules both play a role in exocytosis; however, their interplay is not understood. Here we study the... Read More about Interaction of microtubules and actin during the post-fusion phase of exocytosis.

SPARC 2019 Fake news & home truths : Salford postgraduate annual research conference book of abstracts (2019)
Book
Aarons, T., Abduljawad, S. A. S., Aboelkheir, A., Addis, M. C., Adeboye, O., Adeniyi, I. A., …Yusup, N. H. (2019). E. M. Barnes, D. Greensmith, M. Griffiths, U. Hurley, T. Ireland, C. Starbuck, …D. Whitnall (Eds.), SPARC 2019 Fake news & home truths : Salford postgraduate annual research conference book of abstracts. Salford, UK: University of Salford

Welcome to the Book of Abstracts for the 2019 SPARC conference. This year we not only celebrate the work of our PGRs but also our first ever Doctoral School Best Supervisor awards, which makes this year’s conference extra special. Once again we have... Read More about SPARC 2019 Fake news & home truths : Salford postgraduate annual research conference book of abstracts.

What can independent research for Mesothelioma achieve to treat this orphan disease? (2019)
Journal Article
Guazzelli, A., Meysami, P., Bakker, E., Bonanni, E., Demonacos, C., Krstic- Demonacos, M., & Mutti, L. (2019). What can independent research for Mesothelioma achieve to treat this orphan disease?. Expert Opinion on Investigational Drugs, 28(8), 719-732. https://doi.org/10.1080/13543784.2019.1638363

Introduction: Malignant pleural mesothelioma (MPM) is a rare neoplasm with a poor prognosis, as current therapies are ineffective. Despite the increased understanding of the molecular biology of mesothelioma, there is still a lack of drugs that drama... Read More about What can independent research for Mesothelioma achieve to treat this orphan disease?.

High frequency of infection of lung cancer patients with the parasite Toxoplasma gondii (2019)
Journal Article
Bajnok, J., Tarabulsi, M., Carlin, H., Bown, K., Southworth, T., Dungwa, J., …Hide, G. (2019). High frequency of infection of lung cancer patients with the parasite Toxoplasma gondii. ERJ Open Research, 5(2), 1-10. https://doi.org/10.1183/23120541.00143-2018

Toxoplasma gondii is an intracellular protozoan parasite that can cause a wide range of clinical conditions, including miscarriage and pneumonia. The global prevalence is 30% in humans but varies locally (e.g. UK is typically 10%). The association be... Read More about High frequency of infection of lung cancer patients with the parasite Toxoplasma gondii.

The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks (2019)
Journal Article
Albelazi, M., Martin, P., Mohammed, S., Mutti, L., Parsons, J., & Elder, R. (2019). The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks. Genes, 10(4), 315. https://doi.org/10.3390/genes10040315

Endonuclease VIII-like (NEIL) 1 and 3 proteins eliminate oxidative DNA base damage and psoralen DNA interstrand crosslinks through initiation of base excision repair. Current evidence points to a DNA replication associated repair function of NEIL1 an... Read More about The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks.

Doxycycline, Azithromycin and vitamin C (DAV) : a potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs) (2019)
Journal Article
Fiorillo, M., Tóth, F., Sotgia, F., & Lisanti, M. (2019). Doxycycline, Azithromycin and vitamin C (DAV) : a potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs). Aging, 11(8), 2202-2216. https://doi.org/10.18632/aging.101905

Here, we devised a new strategy for eradicating cancer stem cells (CSCs), via a "synthetic-metabolic" approach, involving two FDA-approved antibiotics and a dietary vitamin supplement. This approach was designed to induce a "rho-zero-like" phenotype... Read More about Doxycycline, Azithromycin and vitamin C (DAV) : a potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs).

Enhancing evolution : the transhuman case for gene doping (2019)
Book Chapter
Miah, A. (2019). Enhancing evolution : the transhuman case for gene doping. In J. Lightfoot, M. Hubal, & S. Roth (Eds.), The Routledge Handbook of Sport and Exercise Systems Genetics (463-474). Abingdon, UK: Routledge

On the high structural heterogeneity of Fe impregnated graphite-carbon catalysts from Fe nitrate precursor (2019)
Journal Article
Arrigo, R., & Schuster, M. (2019). On the high structural heterogeneity of Fe impregnated graphite-carbon catalysts from Fe nitrate precursor. Catalysts, 9(4), 303. https://doi.org/10.3390/catal9040303

Wet impregnation is broadly applied for the synthesis of carbon supported metal/metal oxide nanostructures because of its high flexibility, simplicity and low cost. By contrast, impregnated catalysts are typified by a usually undesired nanostructural... Read More about On the high structural heterogeneity of Fe impregnated graphite-carbon catalysts from Fe nitrate precursor.

The association between Toxoplasma gondii infection and postpartum blues (2019)
Journal Article
Gao, J., He, Z., Xie, Y., Hide, G., Lai, D., & Lun, Z. (2019). The association between Toxoplasma gondii infection and postpartum blues. Journal of Affective Disorders, 250, 404-409. https://doi.org/10.1016/j.jad.2019.03.013

Introduction and Aim: Toxoplasma gondii is an intracellular protozoan parasite infecting approximately 30% of the global human population. It has often been suggested that chronic infection with T. gondii is related to personality changes and vario... Read More about The association between Toxoplasma gondii infection and postpartum blues.

Hallmarks of the cancer cell of origin : comparisons with "energetic" cancer stem cells (e-CSCs) (2019)
Journal Article
Sotgia, F., Fiorillo, M., & Lisanti, M. (2019). Hallmarks of the cancer cell of origin : comparisons with "energetic" cancer stem cells (e-CSCs). Aging, 11(3), 1065-1068. https://doi.org/10.18632/aging.101822

Here, we discuss the expected hallmark(s) of the cancer cell of origin and how this may be related to a new tumor cell phenotype, namely "energetic" cancer stem cells (e-CSCs). e-CSCs show many features that would be characteristic of the cancer cell... Read More about Hallmarks of the cancer cell of origin : comparisons with "energetic" cancer stem cells (e-CSCs).

“Energetic” cancer stem cells (e-CSCs) : a new hyper-metabolic and proliferative tumor cell phenotype, driven by mitochondrial energy (2019)
Journal Article
Fiorillo, M., Sotgia, F., & Lisanti, M. (2019). “Energetic” cancer stem cells (e-CSCs) : a new hyper-metabolic and proliferative tumor cell phenotype, driven by mitochondrial energy. Frontiers in Oncology, 8(677), https://doi.org/10.3389/fonc.2018.00677

Here, we provide the necessary evidence that mitochondrial metabolism drives the anchorage-independent proliferation of CSCs. Two human breast cancer cell lines, MCF7 [ER(+)] and MDA-MB-468 (triple-negative), were used as model systems. To directly... Read More about “Energetic” cancer stem cells (e-CSCs) : a new hyper-metabolic and proliferative tumor cell phenotype, driven by mitochondrial energy.

Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma lewisi, a zoonotic pathogen (2019)
Journal Article
Zhang, X., Li, S., Li, Z., He, C., Hide, G., Lai, D., & Lun, Z. (2019). Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma lewisi, a zoonotic pathogen. Cell Cycle, 18(5), 552-567. https://doi.org/10.1080/15384101.2019.1577651

Trypanosoma (Herpetosoma) lewisi is a globally distributed rat trypanosome, currently considered as a zoonotic pathogen; however, a detailed understanding of the morphological events occurring during the cell cycle is lacking. This study aimed to inv... Read More about Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma lewisi, a zoonotic pathogen.

The effects of inflammation, aging and oxidative stress on the pathogenesis of diabetes mellitus (type 2 diabetes) (2019)
Journal Article
Halim, M., & Halim, A. (2019). The effects of inflammation, aging and oxidative stress on the pathogenesis of diabetes mellitus (type 2 diabetes). Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 13(2), 1165-1172. https://doi.org/10.1016/j.dsx.2019.01.040

Diabetes mellitus is seen to be prevalent among the different epidemics. The prevalence rate of the diabetes mellitus is seen to be increasing in different regions of the world. Type 2 diabetes mellitus is the most common form of the disease that cau... Read More about The effects of inflammation, aging and oxidative stress on the pathogenesis of diabetes mellitus (type 2 diabetes).

BAP1 status determines the sensitivity of malignant mesothelioma cells to gemcitabine treatment (2019)
Journal Article
Guazzelli, A., Meysami, P., Bakker, E., Demonacos, C., Giordano, A., Krstic- Demonacos, M., & Mutti, L. (2019). BAP1 status determines the sensitivity of malignant mesothelioma cells to gemcitabine treatment. International Journal of Molecular Sciences, 20(2), 429. https://doi.org/10.3390/ijms20020429

Malignant mesothelioma (MMe) is a cancer with poor prognosis and resistance to standard treatments. Recent reports have highlighted the role of the BRCA associated protein 1 gene BAP1 in the development of MMe. In this study, the chemosensitivity of... Read More about BAP1 status determines the sensitivity of malignant mesothelioma cells to gemcitabine treatment.

The ER-alpha mutation Y537S confers Tamoxifen-resistance via enhanced mitochondrial metabolism, glycolysis and Rho-GDI/PTEN signaling : implicating TIGAR in somatic resistance to endocrine therapy (2018)
Journal Article
Fiorillo, M., Sanchez-Alvarez, R., Sotgia, F., & Lisanti, M. (2018). The ER-alpha mutation Y537S confers Tamoxifen-resistance via enhanced mitochondrial metabolism, glycolysis and Rho-GDI/PTEN signaling : implicating TIGAR in somatic resistance to endocrine therapy. Aging, 10(12), 4000-4023. https://doi.org/10.18632/aging.101690

Naturally-occurring somatic mutations in the estrogen receptor gene (ESR1) have been previously implicated in the clinical development of resistance to hormonal therapies, such as Tamoxifen. For example, the somatic mutation Y537S has been specifical... Read More about The ER-alpha mutation Y537S confers Tamoxifen-resistance via enhanced mitochondrial metabolism, glycolysis and Rho-GDI/PTEN signaling : implicating TIGAR in somatic resistance to endocrine therapy.

Landscape structure affects the prevalence and distribution of a tick-borne zoonotic pathogen (2018)
Journal Article
Millins, C., Dickinson, E., Isakovic, P., Gilbert, L., Wojciechowska, A., Paterson, V., …Biek, R. (2018). Landscape structure affects the prevalence and distribution of a tick-borne zoonotic pathogen. Parasites and Vectors, 11(2018), 621. https://doi.org/10.1186/s13071-018-3200-2

Background Landscape structure can affect pathogen prevalence and persistence with consequences for human and animal health. Few studies have examined how reservoir host species traits may interact with landscape structure to alter pathogen communi... Read More about Landscape structure affects the prevalence and distribution of a tick-borne zoonotic pathogen.

The role of the copper oxidation state in the electrocatalytic reduction of CO2 into valuable hydrocarbons (2018)
Journal Article
Velasco-Vélez, J., Jones, T., Gao, D., Carbonio, E., Arrigo, R., Hsu, C., …Chuang, C. (2018). The role of the copper oxidation state in the electrocatalytic reduction of CO2 into valuable hydrocarbons. ACS sustainable chemistry & engineering, 7(1), 1485-1492. https://doi.org/10.1021/acssuschemeng.8b05106

Redox-active copper catalysts with accurately prepared oxidation states (Cu0, Cu+ and Cu2+) and high selectivity to C2 hydrocarbon formation, from electrocatalytic cathodic reduction of CO2, were fabricated and characterized. The electrochemically pr... Read More about The role of the copper oxidation state in the electrocatalytic reduction of CO2 into valuable hydrocarbons.

Azithromycin and Roxithromycin define a new family of “senolytic” drugs that target senescent human fibroblasts (2018)
Journal Article
Ozsvari, B., Nuttall, J., Sotgia, F., & Lisanti, M. (2018). Azithromycin and Roxithromycin define a new family of “senolytic” drugs that target senescent human fibroblasts. Aging, 10(11), 3294-3307. https://doi.org/10.18632/aging.101633

Here, we employed a “senolytic” assay system as a screening tool, with the goal of identifying and repurposing FDA-approved antibiotics, for the targeting of the senescent cell population. Briefly, we used two established human fibroblast cell lines... Read More about Azithromycin and Roxithromycin define a new family of “senolytic” drugs that target senescent human fibroblasts.

Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability (2018)
Journal Article
Doherty, J., Raoof, A., Hussain, A., Wolna, M., Cinque, G., Brown, M., …Denbigh, J. (2019). Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability. Analyst, 144(3), 997-1007. https://doi.org/10.1039/C8AN01566J

Synchrotron radiation Fourier transform infrared microspectroscopy (SR-microFTIR) of live biological cells has the potential to provide far greater biochemical and morphological detail than equivalent studies using dehydrated, chemically-fixed single... Read More about Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability.