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Prof Geoff Hide's Outputs (113)

A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper. (2003)
Journal Article
G, H., JM, H., & R, M. (2003). A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper. BMC Ecology, 3(7), https://doi.org/10.1186/1472-6785-3-7

Background
The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum... Read More about A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper..

Trypanosoma brucei : trypanosome strain typing using PCR analysis of mobile genetic elements (MGE-PCR) (2003)
Journal Article
Tilley, A., Welburn, S., Fevre, E., Feil, E., & Hide, G. (2003). Trypanosoma brucei : trypanosome strain typing using PCR analysis of mobile genetic elements (MGE-PCR). Experimental Parasitology, 104(1-2), 26-32. https://doi.org/10.1016/S0014-4894%2803%2900114-0

We describe the development of a single-primer amplification system, which uses the trypanosomal mobile genetic element RIME as a molecular marker for the differentiation of Trypanosoma brucei stocks. Using a well-characterised set of T. brucei stock... Read More about Trypanosoma brucei : trypanosome strain typing using PCR analysis of mobile genetic elements (MGE-PCR).

The molecular epidemiology of Toxoplasma gondii with specific reference to transmissions in sheep (2003)
Thesis
Duncanson, P. The molecular epidemiology of Toxoplasma gondii with specific reference to transmissions in sheep. (Thesis). Salford : University of Salford

Much research has been conducted on the life cycle of T. gondii by means of
investigation of experimentally infected animals. However, much less has been
achieved in studies of naturally infected populations. In particular, controversy
surrounds t... Read More about The molecular epidemiology of Toxoplasma gondii with specific reference to transmissions in sheep.

Development of PCR based techniques for the characterisation of Trypanosoma brucei strains from East Africa (2002)
Thesis
Tilley, A. Development of PCR based techniques for the characterisation of Trypanosoma brucei strains from East Africa. (Thesis). Salford : University of Salford

Human trypanosomiasis in east Africa, caused by Trypanosoma brucei rhodesiense,
occurs in specific foci that alternate between endemic and epidemic states.
Identification of T.b. rhodesiense is complicated as it also circulates in cattle
rese... Read More about Development of PCR based techniques for the characterisation of Trypanosoma brucei strains from East Africa.

High levels of congenital transmission of toxoplasma gondii in a commercial sheep flock (2001)
Journal Article
Duncanson, P., Terry, R., Smith, J., & Hide, G. (2001). High levels of congenital transmission of toxoplasma gondii in a commercial sheep flock. International Journal for Parasitology, 31(14), 1699-1703. https://doi.org/10.1016/S0020-7519%2801%2900282-X

Our current understanding of congenital transmission of Toxoplasma gondii from ewe to lamb dictates that infection frequently results in abortion and the death of the developing foetus, that the birth of live infected lambs occurs rarely and that the... Read More about High levels of congenital transmission of toxoplasma gondii in a commercial sheep flock.

MGE-PCR : a novel approach to the analysis of toxoplasma gondii strain differentiation using mobile genetic elements (2001)
Journal Article
Terry, R., Smith, J., Duncanson, P., & Hide, G. (2001). MGE-PCR : a novel approach to the analysis of toxoplasma gondii strain differentiation using mobile genetic elements. International Journal for Parasitology, 31(2), 155-161. https://doi.org/10.1016/S0020-7519%2800%2900158-2

The position of mobile genetic elements (MGE) within eukaryotic genomes is often highly variable and we have exploited this phenomenon to develop a novel approach to strain differentiation in Toxoplasma gondii. Two PCR based strategies were designed... Read More about MGE-PCR : a novel approach to the analysis of toxoplasma gondii strain differentiation using mobile genetic elements.

Generation of a mosaic pattern of diversity in the major merozoite-piroplasm surface antigen of Theileria annulata (2000)
Journal Article
Gubbels, M.-J., Katzer, F., Hide, G., Jongejan, F., & R Shiels, B. (2000). Generation of a mosaic pattern of diversity in the major merozoite-piroplasm surface antigen of Theileria annulata. Molecular and Biochemical Parasitology, 110(1), 23-32. https://doi.org/10.1016/s0166-6851%2800%2900253-x

The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata. Generation and selection of divergent antigenic types has implications for the inclusion of the Tams1 antigen in a subu... Read More about Generation of a mosaic pattern of diversity in the major merozoite-piroplasm surface antigen of Theileria annulata.

History of sleeping sickness in East Africa. (1999)
Journal Article
G, H. (1999). History of sleeping sickness in East Africa. Clinical Microbiology Reviews, 12, 112-25. https://doi.org/10.1128/CMR.12.1.112

The history of human sleeping sickness in East Africa is characterized by the appearance of disease epidemics interspersed by long periods of endemicity. Despite the presence of the tsetse fly in large areas of East Africa, these epidemics tend to oc... Read More about History of sleeping sickness in East Africa..

The molecular epidemiology of parasites (1991)
Journal Article
G, H., & A, T. (1991). The molecular epidemiology of parasites. #Journal not on list, 47, 128–142. https://doi.org/10.1007/BF01945413

The explosion of new techniques, made available by the rapid advance in molecular biology, has provided a battery of novel approaches and technology which can be applied to more practical issues such as the epidemiology of parasites. In this review,... Read More about The molecular epidemiology of parasites.

The identification of Trypanosoma brucei subspecies using repetitive DNA sequences (1990)
Journal Article
Hide, G., Cattand, P., LeRay, D., Barry, J., & Tait, A. (1990). The identification of Trypanosoma brucei subspecies using repetitive DNA sequences. Molecular and Biochemical Parasitology, 39(2), 213-225. https://doi.org/10.1016/0166-6851%2890%2990060-y

We describe the use of repetitive DNA probes to characterise the relationships between different stocks of African trypanosomes representing the subspecies of Trypanosoma brucei. Probes derived from the ribosomal RNA genes (coding region and non-tran... Read More about The identification of Trypanosoma brucei subspecies using repetitive DNA sequences.

Identification of an epidermal growth factor receptor homologue in trypanosomes (1989)
Journal Article
Hide, G., Gray, A., Harrison, C. M., & Tait, A. (1989). Identification of an epidermal growth factor receptor homologue in trypanosomes. Molecular and Biochemical Parasitology, 36(1), 51-59. https://doi.org/10.1016/0166-6851%2889%2990199-0

Considerable advances have been made in our understanding of cell growth regulation in mammalian cells. In particular, studies on transformed and normal cells have highlighted the contribution of growth factor-related control mechanisms in cell growt... Read More about Identification of an epidermal growth factor receptor homologue in trypanosomes.