Use of a compound having two gold (1) atoms each covalently bonded to a carbon atom in a covalent link connecting the two gold (1) atoms for the treatment of cancer (2005)
Patent
McGown, A., & Flower, K. (2005). Use of a compound having two gold (1) atoms each covalently bonded to a carbon atom in a covalent link connecting the two gold (1) atoms for the treatment of cancer

The invention relates to the treatment of cancers, in particular, cancers that are resistant to platinum based chemotherapeutic agents. A pharmaceutical composition for the treatment of cancer comprising an effective amount of a compound having two g... Read More about Use of a compound having two gold (1) atoms each covalently bonded to a carbon atom in a covalent link connecting the two gold (1) atoms for the treatment of cancer.

Preclinical evaluation of the pharmacodynamic properties of 2,5-diaziridinyl-3-hydroxymethyl-6-methyl-1,4-benzoquinone (2005)
Journal Article
Ward, T., Danson, S., McGown, A., Ranson, M., Coe, N., Jayson, G., …Butler, J. (2005). Preclinical evaluation of the pharmacodynamic properties of 2,5-diaziridinyl-3-hydroxymethyl-6-methyl-1,4-benzoquinone. Clinical Cancer Research, 11(7), 2695-2701. https://doi.org/10.1158/1078-0432.CCR-04-1751

Purpose: The purpose of our study was to investigate the cellular accumulation, DNA cross-linking ability, and cellular toxicity of RH1 (2,5-diaziridinyl-3-[hydroxymethyl[-6-methyl-1,4-benzoquinone), a novel DNA alkylating agent currently in clinical... Read More about Preclinical evaluation of the pharmacodynamic properties of 2,5-diaziridinyl-3-hydroxymethyl-6-methyl-1,4-benzoquinone.

Synthesis and evaluation of double bond substituted combretastatins (2005)
Journal Article
Hadfield, J., Gaukroger, K., Hirst, N., Weston, A., Lawrence, N., & McGown, A. (2005). Synthesis and evaluation of double bond substituted combretastatins. European Journal of Medicinal Chemistry, 40(6), 529-541. https://doi.org/10.1016/j.ejmech.2004.12.008

A series of combretastatins substituted with epoxides, amides and small alkyl groups has been synthesised and evaluated for cytotoxicity and their ability to inhibit the assembly of tubulin. The methyl and ethyl substituted phenols 36, 44 have shown... Read More about Synthesis and evaluation of double bond substituted combretastatins.

Identification and reactivity of the triplet excited state of 5-hydroxytryptophan (2005)
Journal Article
Dad, S., Bisby, R., Clark, I., & Parker, A. (2005). Identification and reactivity of the triplet excited state of 5-hydroxytryptophan. Journal of Photochemistry and Photobiology B: Biology, 78(3), 245-251. https://doi.org/10.1016/j.jphotobiol.2004.11.013

Both the neurotransmitter serotonin and the unnatural amino acid 5-hydroxytryptophan (5HT), contain the 5-hydroxyindole chromophore. The photochemistry of 5HT is being investigated in relation to the multiphoton excitation of this chromophore to prod... Read More about Identification and reactivity of the triplet excited state of 5-hydroxytryptophan.

Human monocyte isolation methods influence cytokine production from in vitro generated dendritic cells (2005)
Journal Article
Elkord, E., Williams, P., Kynaston, H., & Rowbottom, A. (2005). Human monocyte isolation methods influence cytokine production from in vitro generated dendritic cells. Immunology, 114(2), 204-212. https://doi.org/10.1111/j.1365-2567.2004.02076.x

There is growing interest in the in vitro generation of dendritic cells (DC) from peripheral blood monocytes, but the effect of the method chosen to isolate CD14+ monocytes for subsequent DC generation is poorly documented. The method used to isolate... Read More about Human monocyte isolation methods influence cytokine production from in vitro generated dendritic cells.

Differential CTLs specific for prostate-specific antigen in healthy donors and patients with prostate cancer (2005)
Journal Article
Elkord, E., Williams, P., Kynaston, H., & Rowbottom, A. (2005). Differential CTLs specific for prostate-specific antigen in healthy donors and patients with prostate cancer. International Immunology, 17(10), 1315-1325. https://doi.org/10.1093/intimm/dxh309

Induction of CTL responses specific for prostate-specific antigen (PSA)-derived peptides in healthy individuals and patients with prostate cancer (PC) was investigated. Eight PSA-derived peptides that have the potential to bind HLA-A2 molecules were... Read More about Differential CTLs specific for prostate-specific antigen in healthy donors and patients with prostate cancer.

Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation (2005)
Journal Article
Mabley, J., Pacher, P., Deb, A., Wallace, R., Elder, R., & Szabó, C. (2005). Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation. FASEB Journal, 19(2), 290-2. https://doi.org/10.1096/fj.04-2278fje

OGG-1 DNA glycosylase (OGG-1) is an enzyme involved in DNA repair. It excises 7,8-dihydro-8-oxoguanine, which is formed by oxidative damage of guanine. We have investigated the role of OGG-1 in inflammation using three models of inflammation: endotox... Read More about Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation.

Distribution and clinical significance of heparan sulfate proteoglycans in ovarian cancer (2004)
Journal Article
Davies, E., Blackhall, F., Shanks, J., David, G., McGown, A., Swindell, R., …Jayson, G. (2004). Distribution and clinical significance of heparan sulfate proteoglycans in ovarian cancer. Clinical Cancer Research, 10(15), 5178-5186. https://doi.org/10.1158/1078-0432.CCR-03-0103

Purpose: Heparan sulfate proteoglycans have been implicated in cancer cell growth, invasion, metastasis, and angiogenesis. This study was designed to compare their expression in normal ovary and ovarian tumors and then to examine their prognostic sig... Read More about Distribution and clinical significance of heparan sulfate proteoglycans in ovarian cancer.

Asymmetric alkylation of diarylmethane derivatives. Improved results using methoxyethoxy substituent (2004)
Journal Article
Wilkinson, J., Rossington, S., Leonard, J., & Hussain, N. (2004). Asymmetric alkylation of diarylmethane derivatives. Improved results using methoxyethoxy substituent. Tetrahedron Letters, 45(28), 5481-5483. https://doi.org/10.1016/j.tetlet.2004.05.058

Alkylation of 2-methoxyethoxyphenyl phenyl methane using sec-BuLi and (−)-sparteine has been carried out in excellent yields and up to 94% ee. The best results were obtained in allylation reactions but methylation, ethylation, benzylation and trimeth... Read More about Asymmetric alkylation of diarylmethane derivatives. Improved results using methoxyethoxy substituent.

Nematode Aminopeptidases (2004)
Book Chapter
Brooks, D., & Isaac, R. (2004). Nematode Aminopeptidases. In N. Hooper, & U. Lendeckel (Eds.), Aminopeptidases in Biology and Disease (309-329). New York: Springer

The free-living nematode Caenorhabditis elegans has proven to be an excellent model organism for studying animal development and neurobiology. Analysis of the fully sequenced genome of C. elegans predicts that there are ~19,000 genes, of which ~ 2% a... Read More about Nematode Aminopeptidases.

Asymmetric alkylation of diphenylmethane derivatives using (-)-sparteine (2004)
Journal Article
Wilkinson, J., Rossington, S., Leonard, J., & Hussain, N. (2004). Asymmetric alkylation of diphenylmethane derivatives using (-)-sparteine. Tetrahedron Letters, 45(6), 1191-1193. https://doi.org/10.1016/j.tetlet.2003.11.138

Alkylation of 2-oxygenated diphenylmethane derivatives using sec-butyllithium and (−)-sparteine gave enantiomeric excesses of up to 60% with allyl bromide but alkylations with methyl electrophiles were poorly selective. When compounds with a free hyd... Read More about Asymmetric alkylation of diphenylmethane derivatives using (-)-sparteine.

Diblock polyampholytes grafted onto spherical particles : Monte Carlo simulation and lattice mean-field theory (2004)
Journal Article
Akinchina, A., Shusharina, N., & Linse, P. (2004). Diblock polyampholytes grafted onto spherical particles : Monte Carlo simulation and lattice mean-field theory. Langmuir, 20(23), 10351-10360. https://doi.org/10.1021/la0490386

Spherical brushes composed of diblock polyampholytes (diblock copolymers with oppositely charged blocks) grafted onto solid spherical particles in aqueous solution are investigated by using the primitive model solved with Monte Carlo simulations an... Read More about Diblock polyampholytes grafted onto spherical particles : Monte Carlo simulation and lattice mean-field theory.

The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway (2004)
Journal Article
Gros, L., Ishchenko, A., Ide, H., Elder, R., & Saparbaev, M. (2004). The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway. Nucleic Acids Research, 32(1), 73-81. https://doi.org/10.1093/nar/gkh165

In nucleotide incision repair (NIR), an endonuclease nicks oxidatively damaged DNA in a DNA glycosylase-independent manner, providing the correct ends for DNA synthesis coupled to the repair of the remaining 5'-dangling modified nucleotide. This mech... Read More about The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway.

An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site (2003)
Journal Article
Brooks, D., Appleford, P., Murray, L., & Isaac, R. (2003). An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site. Journal of Biological Chemistry, 278(52), 52340-52346. https://doi.org/10.1074/jbc.M308858200

Genome sequence analyses predict many proteins that are structurally related to proteases but lack catalytic residues, thus making functional assignment difficult. We show that one of these proteins (ACN-1), a unique multi-domain angiotensin-converti... Read More about An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site.

Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate (2003)
Journal Article
Barbin, A., Wang, R., O'Connor, P., & Elder, R. (2003). Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate. Cancer Research, 63(22), 7699-703

Ethenobases are promutagenic DNA adducts formed by some environmental carcinogens and products of endogenous lipid peroxidation. Mutation spectra in tumors induced in mice by urethane or its metabolite vinyl carbamate (Vcar) are compatible with 1,N(6... Read More about Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate.

The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction (2003)
Journal Article
Brooks, D., Hooper, N., & Isaac, R. (2003). The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction. Journal of Biological Chemistry, 278(44), 42795-42801. https://doi.org/10.1074/jbc.M306216200

Mammals possess membrane-associated and cytosolic forms of the puromycin-sensitive aminopeptidase (PSA; EC 3.4.11.14). Increasing evidence suggests the membrane PSA is involved in neuromodulation within the central nervous system and in reproductive... Read More about The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction.

DNA N-glycosylase deficient mice: a tale of redundancy (2003)
Journal Article
Parsons, J., & Elder, R. (2003). DNA N-glycosylase deficient mice: a tale of redundancy. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 531(1-2), 165-75. https://doi.org/10.1016/j.mrfmmm.2003.05.001

The generation of mouse models of base excision repair deficiency has resulted in a re-examination of the cellular defence mechanisms that exist to counteract oxidative base damage. Contrary to exhibiting various detrimental effects of the gene disru... Read More about DNA N-glycosylase deficient mice: a tale of redundancy.

Validation of a novel ELISA for measurement of MDA-LDL in human plasma (2003)
Journal Article
Bevan, R., Durand, M., Hickenbotham, P., Kitas, G., Patel, P., Podmore, I., …Lunec, J. (2003). Validation of a novel ELISA for measurement of MDA-LDL in human plasma. Free Radical Biology and Medicine, 35(5), 517-527. https://doi.org/10.1016/S0891-5849%2803%2900359-9

The involvement of oxidatively modified low density lipoprotein (LDL) in the development of CHD is widely described. We have produced two antibodies, recognizing the lipid oxidation product malondialdehyde (MDA) on whole LDL or ApoB-100. The antibodi... Read More about Validation of a novel ELISA for measurement of MDA-LDL in human plasma.

Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit? (2003)
Journal Article
Gaukroger, K., Hadfield, J., Lawrence, N., Nolan, S., & McGown, A. (2003). Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit?. Organic and Biomolecular Chemistry, 1(17), 3033-3037. https://doi.org/10.1039/b306878a

A series of combretastatins possessing both a trimethoxy unit and other substituents on ring A has been synthesised and tested for cytotoxicity and their ability to interact with the protein tubulin. All previous studies have indicated that the trime... Read More about Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit?.

Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers (2003)
Journal Article
Karakoula, A., Evans, M., Podmore, I., Hutchinson, P., Lunec, J., & Cooke, M. (2003). Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers. Journal of Immunological Methods, 277(1-2), 27-37. https://doi.org/10.1016/S0022-1759%2803%2900122-4

The induction and repair of DNA damage has been shown to occur heterogeneously throughout the mammalian genome. As a consequence, analysis of these parameters at a global genome level may not reflect important gene-level events. Few techniques have b... Read More about Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers.