Discovery of novel triple helical DNA intercalators by an integrated virtual and actual screening platform (2009)
Journal Article
Holt, A., Ragazzon, P., Strekowski, L., Chaires, J., & Trent, J. (2009). Discovery of novel triple helical DNA intercalators by an integrated virtual and actual screening platform. Nucleic Acids Research, 37(4), 1280-1287. https://doi.org/10.1093/nar/gkn1043

Virtual Screening is an increasingly attractive way to discover new small molecules with potential medicinal value. We introduce a novel strategy that integrates use of the molecular docking software Surflex with experimental validation by the method... Read More about Discovery of novel triple helical DNA intercalators by an integrated virtual and actual screening platform.

A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles (2009)
Journal Article
Krpetic, Z., Nativo, P., Porta, F., & Brust, M. (2009). A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles. Bioconjugate Chemistry, 20(3), 619-624. https://doi.org/10.1021/bc8003028

Gold nanoparticles of two different sizes stabilized by a 15-mer peptide ligand specifically designed for this purpose have been prepared in aqueous solution and characterized by UV−vis spectroscopy and TEM. The presence of the ligand and its binding... Read More about A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles.

A theoretical self-consistent field study of mixed interfacial biopolymer films (2009)
Book Chapter
Ettelaie, R., Akinshina, A., & Dickinson, E. (2009). A theoretical self-consistent field study of mixed interfacial biopolymer films. In Micro/Nanoencapsulation of Active Food Ingredients (46-66). ACS Publications. https://doi.org/10.1021/bk-2009-1007.ch003

The adsorption of a linear polyelectrolyte onto an existing layer of protein at an interface has been investigated. Calculations using a simple model, involving only the short range interactions, show that the polyelectrolyte forms a more extended di... Read More about A theoretical self-consistent field study of mixed interfacial biopolymer films.

Electroreductions on silver-based electrocatalysts : the use of Ag Nanoparticles for CHCl3to CH4Conversion (2009)
Journal Article
Rondinini, S., Aricci, G., Krpetic, Z., Locatelli, C., Minguzzi, A., Porta, F., & Vertova, A. (2009). Electroreductions on silver-based electrocatalysts : the use of Ag Nanoparticles for CHCl3to CH4Conversion. Fuel Cells, 9(3), 253-263. https://doi.org/10.1002/fuce.200800083

A preliminary investigation on a new class on electrocatalytic materials for the electroreduction of organic halides is presented and discussed. The electrocatalysts are based on silver nanoparticles (Ag_NP), ad hoc synthesised by chemical reduct... Read More about Electroreductions on silver-based electrocatalysts : the use of Ag Nanoparticles for CHCl3to CH4Conversion.

MafB restricts M-CSF-Dependent myeloid commitment divisions of hematopoietic stem cells (2009)
Journal Article
Sarrazin, S., Mossadegh-Keller, N., Fukao, T., Aziz, A., Mourcin, F., Vanhille, L., …Sieweke, M. (2009). MafB restricts M-CSF-Dependent myeloid commitment divisions of hematopoietic stem cells. Cell, 138(2), 300-313. https://doi.org/10.1016/j.cell.2009.04.057

While hematopoietic stem cell (HSC) self-renewal is well studied, it remains unknown whether distinct control mechanisms enable HSC divisions that generate progeny cells with specific lineage bias. Here, we report that the monocytic transcription fac... Read More about MafB restricts M-CSF-Dependent myeloid commitment divisions of hematopoietic stem cells.

MafB/c-Maf deficiency enables self-renewal of differentiated functional macrophages (2009)
Journal Article
Aziz, A., Soucie, E., Sarrazin, S., & Sieweke, M. (2009). MafB/c-Maf deficiency enables self-renewal of differentiated functional macrophages. Science, 326(5954), 867-871. https://doi.org/10.1126/science.1176056

In metazoan organisms, terminal differentiation is generally tightly linked to cell cycle exit, whereas the undifferentiated state of pluripotent stem cells is associated with unlimited self-renewal. Here, we report that combined deficiency for the t... Read More about MafB/c-Maf deficiency enables self-renewal of differentiated functional macrophages.

Intracellular molecular imaging using multiphoton-excited microscopy (2009)
Thesis
Crisostomo, A. Intracellular molecular imaging using multiphoton-excited microscopy. (Thesis). Salford : University of Salford

Detection of the levels of serotonin (5-HT) present in biofluids may be used for detection of neurological diseases such as Alzheimer's 1 . This project seeks to develop technologies using multiphoton excitation and resultant luminescence of serot... Read More about Intracellular molecular imaging using multiphoton-excited microscopy.

Investigation into pregnancy and toxoplasma gondii infection in Libya (2009)
Thesis
Abushahma, M. Investigation into pregnancy and toxoplasma gondii infection in Libya. (Thesis). University of Salford

Toxoplasma gone/// is a parasite discovered in 1908 by Nicolle and Manceaux; they found a protozoan in tissue of a hamster-like rodent in laboratory of the Charles Nicolle at the Pasteur Institute in Tunisia. It is an obligate intracellular protozo... Read More about Investigation into pregnancy and toxoplasma gondii infection in Libya.

Helminth species richness in wild wood mice, Apodemus sylvaticus, is enhanced by the presence of the intestinal nematode Heligmosomoides polygyrus (2009)
Journal Article
Behnke, J., Eira, C., Rogan, M., Gilbert, F., Torres, J., Miquel, J., & Lewis, J. (2009). Helminth species richness in wild wood mice, Apodemus sylvaticus, is enhanced by the presence of the intestinal nematode Heligmosomoides polygyrus. Parasitology, 136(07), 793-804. https://doi.org/10.1017/S0031182009006039

We analysed 3 independently collected datasets of fully censused helminth burdens in wood mice, Apodemus sylvaticus, testing the a priori hypothesis of Behnke et al. (2005) that the presence of the intestinal nematode Heligmosomoides polygyrus pred... Read More about Helminth species richness in wild wood mice, Apodemus sylvaticus, is enhanced by the presence of the intestinal nematode Heligmosomoides polygyrus.

Combretastatin-like chalcones as inhibitors of microtubule polymerization. Part 1: synthesis and biological evaluation of antivascular activity. (2009)
Journal Article
Ducki, S., Renninson, D., Woo, M., Kendall, A., Mcgown, A., & Lawrence, N. (2009). Combretastatin-like chalcones as inhibitors of microtubule polymerization. Part 1: synthesis and biological evaluation of antivascular activity. Bioorganic and Medicinal Chemistry, 17(22), 7698-7710. https://doi.org/10.1016/j.bmc.2009.09.039

The alpha-methyl chalcone SD400 is a potent inhibitor of tubulin assembly and possesses potent anticancer activity. Various chalcone analogues were synthesized and evaluated for their cell growth inhibitory properties against the K562 human chronic m... Read More about Combretastatin-like chalcones as inhibitors of microtubule polymerization. Part 1: synthesis and biological evaluation of antivascular activity..

Analogues of Y27632 increase gap junction communication and suppress the formation of transformed NIH3T3 colonies (2009)
Journal Article
Hampson, L., He, X., Hadfield, J., Kemp, T., Butler, J., Mcgown, A., …Hampson, I. (2009). Analogues of Y27632 increase gap junction communication and suppress the formation of transformed NIH3T3 colonies. British Journal of Cancer, 101(5), 829-839. https://doi.org/10.1038/sj.bjc.6605208

Constitutive activation of RhoA-dependent RhoA kinase (ROCK) signalling is known to promote cellular transformation and the ROCK inhibitor Y-27632 has the ability to suppress focus formation of RhoA transformed NIH3T3 cells. METHODS: Sixty-four novel... Read More about Analogues of Y27632 increase gap junction communication and suppress the formation of transformed NIH3T3 colonies.

Biochemical changes and cytotoxicity associated with methionine depletion in paediatric central nervous system tumour cell lines (2009)
Journal Article
Najim, N., Podmore, I., Mcgown, A., & Estlin, E. (2009). Biochemical changes and cytotoxicity associated with methionine depletion in paediatric central nervous system tumour cell lines. Anticancer Research, 29(8), 2971-2976

The aim of this study was to investigate the importance of the extent and duration of methionine depletion as a cause of cytotoxicity for CNS tumour cell lines, and also to investigate the associated in vitro cellular biochemical responses. MATERIALS... Read More about Biochemical changes and cytotoxicity associated with methionine depletion in paediatric central nervous system tumour cell lines.

Methionine restriction reduces the chemosensitivity of central nervous system tumour cell lines (2009)
Journal Article
Najim, N., Podmore, I., Mcgown, A., & Estlin, E. (2009). Methionine restriction reduces the chemosensitivity of central nervous system tumour cell lines. Anticancer Research, 29(8), 3101-3108

The aim of this study was to investigate the effects of methionine depletion with cytotoxic agents that are potentially influenced by depletion of methionine, and are known to have a role in CNS tumour treatments for children. MATERIALS AND METHODS:... Read More about Methionine restriction reduces the chemosensitivity of central nervous system tumour cell lines.

Gold-ligand interaction studies of water-soluble aminoalcohol capped gold nanoparticles by NMR (2008)
Journal Article
Porta, F., Krpetic, Z., Prati, L., Gaiassi, A., & Scarì, G. (2008). Gold-ligand interaction studies of water-soluble aminoalcohol capped gold nanoparticles by NMR. Langmuir, 24(14), 7061-7064. https://doi.org/10.1021/la8008392

Novel reproducible preparations of gold nanoparticles capped by aminoalcohols have been set up by reduction of HAuCl4 with NaBH4 in aqueous solution. The characterization of Au@aminoalcohol nanoparticles performed by 1H NMR, ATR-FTIR, UV−vis spectros... Read More about Gold-ligand interaction studies of water-soluble aminoalcohol capped gold nanoparticles by NMR.

Effect of O6-methylguanine on the stability of G-quadruplex DNA (2008)
Journal Article
Mekmaysy, C., Petraccome, L., Garbett, N., Ragazzon, P., Gray, R., Trent, J., & Chaires, J. (2008). Effect of O6-methylguanine on the stability of G-quadruplex DNA. Abstracts of papers / American Chemical Society, 130(21), 6710-6711. https://doi.org/10.1021/ja801976h

The effects of substitution of O6-methylguanine on the structure and stability of a human telomere quadruplex was studied by circular dichroism, thermal denaturation, analytical ultracentrifugation, and molecular dynamics simulations. The results sho... Read More about Effect of O6-methylguanine on the stability of G-quadruplex DNA.

Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. (2008)
Journal Article
Davin-Regli, A., Bolla, J., James, C., Lavigne, J., Chevalier, J., Garnotel, E., …Pagès, J. (2008). Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. Current Drug Targets, 9(9), 750-759. https://doi.org/10.2174/138945008785747824

In Enterobacteriaceae, membrane permeability is a key in the level of susceptibility to antibiotics. Modification of the bacterial envelope by decreasing the porin production or increasing the expression of efflux pump systems has been reported. Thes... Read More about Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens..

Adoptive transfer of T(reg) depleted autologous T cells in advanced renal cell carcinoma (2008)
Journal Article
Thistlethwaite, F., Elkord, E., Griffiths, R., Burt, D., Shablak, A., Campbell, J., …Hawkins, R. (2008). Adoptive transfer of T(reg) depleted autologous T cells in advanced renal cell carcinoma. Cancer Immunology, Immunotherapy, 57(5), 623-634. https://doi.org/10.1007/s00262-007-0400-6

PURPOSE: CD4(+)CD25(+) regulatory T (T(reg)) cells are present in increased numbers in patients with advanced cancer and CD25(+) T cell depletion potentiates tumour immunity in animal models. The aim of this study was to assess the feasibility and sa... Read More about Adoptive transfer of T(reg) depleted autologous T cells in advanced renal cell carcinoma.

CD4+ T-cell recognition of human 5T4 oncofoetal antigen: implications for initial depletion of CD25+ T cells (2008)
Journal Article
Elkord, E., Burt, D., Drijfhout, J., Hawkins, R., & Stern, P. (2008). CD4+ T-cell recognition of human 5T4 oncofoetal antigen: implications for initial depletion of CD25+ T cells. Cancer Immunology, Immunotherapy, 57(6), 833-847. https://doi.org/10.1007/s00262-007-0419-8

BACKGROUND: The human 5T4 (h5T4) oncofoetal antigen is expressed by a wide variety of human carcinomas including colorectal, ovarian, gastric and renal, but rarely on normal tissues. Its restricted expression on tumour tissues as well as its associat... Read More about CD4+ T-cell recognition of human 5T4 oncofoetal antigen: implications for initial depletion of CD25+ T cells.

Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy (2008)
Journal Article
Elkord, E., Hawkins, R., & Stern, P. (2008). Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy. Expert Opinion on Biological Therapy, 8(4), 385-395. https://doi.org/10.1517/14712598.8.4.385

BACKGROUND: Despite improvement in conventional strategies for treating gastrointestinal (GI) carcinoma, large numbers of patients still suffer from incurable or progressive disease. OBJECTIVE: Here we consider the prospects for circumventing limi... Read More about Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy.

Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia (2008)
Journal Article
Winters, U., Daayana, S., Lear, J., Tomlinson, A., Elkord, E., Stern, P., & Kitchener, H. (2008). Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia. Clinical Cancer Research, 14(16), 5292-5299. https://doi.org/10.1158/1078-0432.CCR-07-4760

PURPOSE: High-risk human papillomavirus (HPV)-associated vulval intraepithelial neoplasia (VIN) is difficult to treat by excision or ablation because of high recurrence rates. Small studies of photodynamic therapy (PDT) and imiquimod treatments have... Read More about Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia.