Conjugation of PEG and gold nanoparticles to increase the accessibility and valency of tethered RNA splicing enhancers (2013)
Journal Article
Perrett, A., Dickinson, R., Krpetic, Z., Brust, M., Lewis, H., Eperon, I., & Burley, G. (2013). Conjugation of PEG and gold nanoparticles to increase the accessibility and valency of tethered RNA splicing enhancers. Chemical Science, 4(1), 257-265. https://doi.org/10.1039/C2SC20937C

Since alternative splicing patterns are exhibited by most mammalian genes, the functional properties of a gene may be changed for therapeutic purposes by nudging splicing from one pattern to another. Splicing to a refractory exon can be enhanced usin... Read More about Conjugation of PEG and gold nanoparticles to increase the accessibility and valency of tethered RNA splicing enhancers.

microRNAs in cancer (2013)
Journal Article
Di Leva, G., Garofalo, M., & Croce, C. (2014). microRNAs in cancer. https://doi.org/10.1146/annurev-pathol-012513-104715

MicroRNAs are small non coding RNAs that typically inhibit the translation and stability of messanger RNAs (mRNAs), controlling genes involved in cellular processes such as inflammation, cell cycle regulation, stress response, differentiation, apop... Read More about microRNAs in cancer.

High-resolution sizing of monolayer-protected gold clusters by differential centrifugal sedimentation (2013)
Journal Article
Krpetic, Z., Davidson, A., Volk, M., Lévy, R., Brust, M., & Cooper, D. (2013). High-resolution sizing of monolayer-protected gold clusters by differential centrifugal sedimentation. ACS nano, 7(10), 8881-8890. https://doi.org/10.1021/nn403350v

Differential centrifugal sedimentation (DCS) has been applied to accurately size ligand-protected gold hydrosols in the 10 to 50 nm range. A simple protocol is presented to correct for particle density variations due to the presence of the ligand she... Read More about High-resolution sizing of monolayer-protected gold clusters by differential centrifugal sedimentation.

Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis (2013)
Journal Article
Greensmith, D. (2014). Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis. Computer Methods and Programs in Biomedicine, 113(1), 241-250. https://doi.org/10.1016/j.cmpb.2013.09.004

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological info... Read More about Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.

Self-consistent field theory for the interactions between keratin intermediate filaments (2013)
Journal Article
Akinshina, A., Jambon-Puillet, E., Warren, P., & Noro, M. (2013). Self-consistent field theory for the interactions between keratin intermediate filaments. BMC Biophysics, 6(1), 12. https://doi.org/10.1186/2046-1682-6-12

Background: Keratins are important structural proteins found in skin, hair and nails. Keratin Intermediate Filaments are major components of corneocytes, nonviable horny cells of the Stratum Corneum, the outermost layer of skin. It is considered that... Read More about Self-consistent field theory for the interactions between keratin intermediate filaments.

The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes (2013)
Journal Article
Greensmith, D., & Nirmalan, M. (2013). The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes. Physiological Reports, 1(4), e00093. https://doi.org/10.1002/phy2.93

The proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) is associated with myocardial dysfunction observed in sepsis and septic shock. There are two fundamental components to this dysfunction. (1) systolic dysfunction; and (2) diastolic dysf... Read More about The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes.

A molecular and ecological analysis of the trematode plagiorchis elegans in the wood mouse apodemus sylvaticus from a periaquatic ecosystem in the UK (2013)
Journal Article
Boyce, K., Hide, G., Craig, P., Reynolds, C., Hussain, M., Bodell, A., …Rogan, M. (2013). A molecular and ecological analysis of the trematode plagiorchis elegans in the wood mouse apodemus sylvaticus from a periaquatic ecosystem in the UK. Journal of Helminthology, 88(03), 310-320. https://doi.org/10.1017/S0022149X13000199

The prevalence of the digenean Plagiorchis sp. was investigated in a natural wood mouse population (Apodemus sylvaticus) in a periaquatic environment. Classical identification was complemented with the use of molecular differentiation to determine... Read More about A molecular and ecological analysis of the trematode plagiorchis elegans in the wood mouse apodemus sylvaticus from a periaquatic ecosystem in the UK.

Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation (2013)
Journal Article
Johnson, D., Heijman, J., Bode, E., Greensmith, D., van der Linde, H., Abi-Gerges, N., …Volders, P. (2013). Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation. Circulation Research, 112(2), 246-56. https://doi.org/10.1161/CIRCRESAHA.112.275735

RATIONALE Spontaneous Ca(2+) release (SCR) from the sarcoplasmic reticulum can cause delayed afterdepolarizations and triggered activity, contributing to arrhythmogenesis during β-adrenergic stimulation. Excessive beat-to-beat variability of repol... Read More about Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation.

The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells (2013)
Journal Article
Cooley, N., Elder, R., & Povey, A. (2013). The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells. BioMed Research International, 2013, https://doi.org/10.1155/2013/152909

The DNA mismatch repair (MMR) and base excision repair (BER) systems are important determinants of cellular toxicity following exposure to agents that cause oxidative DNA damage. To examine the interactions between these different repair systems, we... Read More about The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.

Antimicrobial activity of novel nanostructured Cu-SiO2 coatings prepared by chemical vapour deposition against hospital related pathogens (2013)
Journal Article
Varghese, S., ElFakhri, S., Sheel, D., Sheel, P., Bolton, F., & Foster, H. (2013). Antimicrobial activity of novel nanostructured Cu-SiO2 coatings prepared by chemical vapour deposition against hospital related pathogens. AMB Express, 2(53), https://doi.org/10.1186/2191-0855-3-53

There is increasing recognition that the healthcare environment acts as an important reservoir for transmission of healthcare acquired infections (HCAI). One method of reducing environmental contamination would be use of antimicrobial materials. The... Read More about Antimicrobial activity of novel nanostructured Cu-SiO2 coatings prepared by chemical vapour deposition against hospital related pathogens.

Human parasitology - book review (2013)
Journal Article
Hide, G. (2013). Human parasitology - book review. British Journal of Biomedical Science, 70(4), 184-184

Myeloid-derived suppressor cells in cancer: recent progress and prospects (2013)
Journal Article
Khaled, Y., Ammori, B., & Elkord, E. (2013). Myeloid-derived suppressor cells in cancer: recent progress and prospects. Immunology and Cell Biology, 91(8), 493-502. https://doi.org/10.1038/icb.2013.29

Immunosuppressive cells, mainly myeloid-derived suppressor cells (MDSCs) and T regulatory cells, downregulate antitumour immunity and cancer immunotherapy. MDSCs are a heterogeneous group of immature myeloid cells that negatively regulate the immune... Read More about Myeloid-derived suppressor cells in cancer: recent progress and prospects.

Novel cyanocombretastatins as potent tubulin polymerisation inhibitors (2012)
Journal Article
Jalily, P., Hirst, N., Hadfield, J., & Rossington, S. (2012). Novel cyanocombretastatins as potent tubulin polymerisation inhibitors. Bioorganic and Medicinal Chemistry Letters, 22(21), 6731-6734. https://doi.org/10.1016/j.bmcl.2012.08.089

A series of novel cyanocombretastatins bearing a 3,4,5-trimethoxyphenyl moiety combined with a variety of substituted phenyl rings, were synthesised and their antitumour activity was evaluated. The Z-cyanocombretastatins were synthesised in a one-ste... Read More about Novel cyanocombretastatins as potent tubulin polymerisation inhibitors.

Identification of a new species of digenean Notocotylus malhamensis n. sp. (Digenea: Notocotylidae) from the bank vole (Myodes glareolus) and the field vole (Microtus agrestis) (2012)
Journal Article
vole (Myodes glareolus) and the field vole (Microtus agrestis). Parasitology, 139(12), 1630-1639. https://doi.org/10.1017/S0031182012000911

Notocotylus malhamensis n. sp. is described from the caecum of the bank vole (Myodes glareolus) and the field vole (Microtus agrestis) from Malham Tarn Nature Reserve in North Yorkshire, UK. In total, 581 specimens were collected from rodents trapp... Read More about Identification of a new species of digenean Notocotylus malhamensis n. sp. (Digenea: Notocotylidae) from the bank vole (Myodes glareolus) and the field vole (Microtus agrestis).

Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged (2012)
Journal Article
Horn, M., Graham, H., Richards, M., Clarke, J., Greensmith, D., Briston, S., …Trafford, A. (2012). Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged. Journal of Molecular and Cellular Cardiology, 53(1), 82-90. https://doi.org/10.1016/j.yjmcc.2012.03.011

The incidence of heart failure (HF) increases with age. This study sought to determine whether aging exacerbates structural and functional remodeling of the myocardium in HF. HF was induced in young (~18 months) and aged sheep (>8 years) by right ven... Read More about Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged.

Mixed protein–polysaccharide interfacial layers : effect of polysaccharide charge distribution (2012)
Journal Article
Ettelaie, R., Akinshina, A., & Maurer, S. (2012). Mixed protein–polysaccharide interfacial layers : effect of polysaccharide charge distribution. Soft Matter, 8(29), 7582. https://doi.org/10.1039/C2SM25803J

The influence of the polysaccharide charge distribution on the structure, thickness, and charge reversal of the interfacial layers, formed by adsorbed positively charged protein and oppositely charged polysaccharide, has been investigated using a lat... Read More about Mixed protein–polysaccharide interfacial layers : effect of polysaccharide charge distribution.

Antimicrobial activity against hospital-related pathogens of dual layer CuO/TiO2 coatings prepared by CVD (2012)
Journal Article
dual layer CuO/TiO2 coatings prepared by CVD. Chemical Vapor Deposition, 18(4-6), 140-146. https://doi.org/10.1002/cvde.201106978

Photocatalytically active films of TiO2/CuO grown by atmospheric pressure thermal (APT)CVD are investigated for activity against multiple antibiotic-resistant pathogens namely, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, vancomy... Read More about Antimicrobial activity against hospital-related pathogens of dual layer CuO/TiO2 coatings prepared by CVD.

Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung (2012)
Journal Article
Kirchner, S., Fothergill, J., Wright, E., James, C., Mowat, E., & Winstanley, C. (2012). Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung. Journal of Visualized Experiments, 64, 1-8. https://doi.org/10.3791/3857

There is growing concern about the relevance of in vitro antimicrobial susceptibility tests when applied to isolates of P. aeruginosa from cystic fibrosis (CF) patients. Existing methods rely on single or a few isolates grown aerobically and plankto... Read More about Use of artificial sputum medium to test antibiotic efficacy against pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung.

Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge (2012)
Journal Article
Rattray, N., Zalloum, W., Mansell, D., Latimer, J., Schwalbe, C., Blake, A., …Freeman, S. (2012). Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge. Chemical Communications, 48, 6393-6395. https://doi.org/10.1039/C2CC32521G

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to t... Read More about Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge.

Directed assembly of DNA-functionalized gold nanoparticles using pyrrole–imidazole polyamides (2012)
Journal Article
Krpetic, Z., Singh, I., Su, W., Guerrini, L., Faulds, K., Burley, G., & Graham, D. (2012). Directed assembly of DNA-functionalized gold nanoparticles using pyrrole–imidazole polyamides. Abstracts of papers / American Chemical Society, 134(20), 8356-8359. https://doi.org/10.1021/ja3014924

Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson–Crick base pairing of single-stranded DNA sequences as a proxy for self-assembly. Although this approach has been utilized in a variety of applications in nan... Read More about Directed assembly of DNA-functionalized gold nanoparticles using pyrrole–imidazole polyamides.