Positively charged silver nanoparticles and their effect on surface-enhanced Raman scattering of dye-labelled oligonucleotides (2012)
Journal Article
van Lierop, D., Krpetic, Z., Guerrini, L., Larmour, I., Dougan, J., Faulds, K., & Graham, D. (2012). Positively charged silver nanoparticles and their effect on surface-enhanced Raman scattering of dye-labelled oligonucleotides. Chemical Communications, 48(66), 8192. https://doi.org/10.1039/C2CC31731A

Improved positively charged nanoparticles are described to provide a simplified SERS substrate for DNA detection. Complete flocculation of the nanoparticles is prevented due to the controlled analyte induced aggregation. This provides a stable aggreg... Read More about Positively charged silver nanoparticles and their effect on surface-enhanced Raman scattering of dye-labelled oligonucleotides.

Importance of Nanoparticle Size in Colorimetric and SERS-Based Multimodal Trace Detection of Ni(II) Ions with Functional Gold Nanoparticles (2012)
Journal Article
Krpetic, Z., Guerrini, L., Larmour, I., Reglinski, J., Faulds, K., & Graham, D. (2012). Importance of Nanoparticle Size in Colorimetric and SERS-Based Multimodal Trace Detection of Ni(II) Ions with Functional Gold Nanoparticles. Small, 8(5), 707-714. https://doi.org/10.1002/smll.201101980

Colorimetric detection of analytes using gold nanoparticles along with surface-enhanced Raman spectroscopy (SERS) are areas of intense research activity since they both offer sensing of very low concentrations of target species. Multimodal detection... Read More about Importance of Nanoparticle Size in Colorimetric and SERS-Based Multimodal Trace Detection of Ni(II) Ions with Functional Gold Nanoparticles.

Mercaptocarborane-capped gold nanoparticles : electron pools and ion traps with switchable hydrophilicity (2012)
Journal Article
Cioran, A., Musteti, A., Teixidor, F., Krpetic, Z., Prior, I., He, Q., …Viñas, C. (2012). Mercaptocarborane-capped gold nanoparticles : electron pools and ion traps with switchable hydrophilicity. Abstracts of papers / American Chemical Society, 134(1), 212-221. https://doi.org/10.1021/ja203367h

A simple single-phase method for the preparation of ca. 2 nm gold nanoparticles capped with mercaptocarborane ligands is introduced. The resultant monolayer protected clusters (MPCs) exhibit redox-dependent solubility and readily phase transfer betwe... Read More about Mercaptocarborane-capped gold nanoparticles : electron pools and ion traps with switchable hydrophilicity.

Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells (2012)
Journal Article
Bisby, R., Botchway, S., Crisostomo, A., Parker, A., & Scherer, K. (2012). Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells. Journal of Spectroscopy, 27(5-6), 533-540. https://doi.org/10.1155/2012/518353

Uptake of the β-blocker drug propranolol by living glial C6 cells has been observed using fluorescence lifetime imaging with two-photon excitation at 630 nm. Both uptake and release of propranolol occur within minutes and are temperature dependent, b... Read More about Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells.

PPARγ as a molecular target of EPA anti-inflammatory activity during TNF-α-impaired skeletal muscle cell differentiation (2012)
Journal Article
Magee, P., Pearson, S., Whittingham-Dowd, J., & Allen, J. (2012). PPARγ as a molecular target of EPA anti-inflammatory activity during TNF-α-impaired skeletal muscle cell differentiation. Journal of Nutritional Biochemistry, 23(11), 1440-1448. https://doi.org/10.1016/j.jnutbio.2011.09.005

Activated skeletal muscle satellite cells facilitate muscle repair or growth through proliferation, differentiation and fusion into new or existing myotubes. Elevated levels of the proinflammatory cytokine tumor necrosis factor-α (TNF-α) impair this... Read More about PPARγ as a molecular target of EPA anti-inflammatory activity during TNF-α-impaired skeletal muscle cell differentiation.

Prevention of artificial dental plaque formation in vitro by plant extracts (2012)
Journal Article
Smullen, J., Finney, M., Storey, D., & Foster, H. (2012). Prevention of artificial dental plaque formation in vitro by plant extracts. Journal of Applied Microbiology, 113(4), 964-973. https://doi.org/10.1111/j.1365-2672.2012.05380.x

Aims A number of previous studies have shown that plant extracts can inhibit formation of dental plaque. The ability of extracts of Rosemarinus officianalis L., Salvia officianalis L., unfermented cocoa, red grape seed and green tea to inhibit plaque... Read More about Prevention of artificial dental plaque formation in vitro by plant extracts.

Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa (2012)
Journal Article
James, C., Fothergill, J., Kalwij, H., Hall, A., Cottell, J., Brockhurst, M., & Winstanley, C. (2012). Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa. BMC Microbiology, 12(216), 1-10. https://doi.org/10.1186/1471-2180-12-216

Background: Pseudomonas aeruginosa is the most common bacterial pathogen infecting the lungs of patients with cystic fibrosis (CF). The Liverpool Epidemic Strain (LES) is transmissible, capable of superseding other P. aeruginosa populations and is... Read More about Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa.

Detection of Trypanosoma lewisi from wild rats in Southern China and its genetic diversity based on the ITS1 and ITS2 sequences (2012)
Journal Article
Tang, H., Lan, Y., Wen, Y., Zhang, X., Desquesnes, M., Yang, T., …Lun, Z. (2012). Detection of Trypanosoma lewisi from wild rats in Southern China and its genetic diversity based on the ITS1 and ITS2 sequences. Infection, Genetics and Evolution, 12(5), 1046-1051. https://doi.org/10.1016/j.meegid.2012.02.018

Trypanosoma lewisi has widely been considered as a non-pathogenic rat trypanosome. However, more and more cases of humans infected with T. lewisi have been reported around the world, indicating that it can infect humans in some undetermined circums... Read More about Detection of Trypanosoma lewisi from wild rats in Southern China and its genetic diversity based on the ITS1 and ITS2 sequences.

Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection (2012)
Journal Article
Gruner, A., Li, Z., Zhao, Z., Zhu, X., Ren, Q., Nie, F., …Lun, Z. (2012). Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection. PLoS ONE, 7(4), e35834. https://doi.org/10.1371/journal.pone.0035834

Toxoplasma gondii infects humans and warm blooded animals causing devastating disease worldwide. It has long been a mystery as to why the peritoneal macrophages of rats are naturally resistant to T. gondii infection while those of mice are not. Her... Read More about Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection.

Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems (2012)
Journal Article
Bisby, R., Botchway, S., Greetham, G., Hadfield, J., Mcgown, A., Parker, A., …Towrie, M. (2012). Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems. Measurement Science and Technology, 23, 084001. https://doi.org/10.1088/0957-0233/23/8/084001

Fluorescence lifetime images of intrinsic fluorescence obtained with two-photon excitation at 630 nm are shown following uptake of a series of E-combretastatins into live cells, including human umbilical vein endothelial cells (HUVECs) that are the t... Read More about Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems.

Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells (2011)
Journal Article
and distribution in live mammalian cells. European Journal of Cancer, 48(12), 1896-1903. https://doi.org/10.1016/j.ejca.2011.11.025

To investigate within live mammalian cells the uptake and disposition of combretastatins, fluorescence lifetime imaging was used with two-photon excitation (2PE). Combretastatin A4 (CA4) and analogues are potential anticancer drugs due to their abili... Read More about Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells.

Energy dependence of gold nanoparticle radiosensitization in plasmid DNA (2011)
Journal Article
McMahon, S., Hyland, W., Brun, E., Butterworth, K., Coulter, J., Douki, T., …Sicard-Roselli, C. (2011). Energy dependence of gold nanoparticle radiosensitization in plasmid DNA. Journal of Physical Chemistry C, 115(41), 20160-20167. https://doi.org/10.1021/jp206854s

Gold nanopa rticles (GNPs) are of considerableinterest for use as a radiosensitizer, because of their biocompat-ibility and their ability to increase dose deposited because oftheir high mass energy absorption coefficient. Their sensitizingproperties ha... Read More about Energy dependence of gold nanoparticle radiosensitization in plasmid DNA.

Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs (2011)
Journal Article
Elkord, E., Sharma, S., Burt, D., & Hawkins, R. (2011). Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs. Clinical Immunology, 140(3), 218-222. https://doi.org/10.1016/j.clim.2011.04.014

Conversion of conventional T cells into T regulatory cells (Tregs) has been proposed as a potential mechanism for Treg expansion in cancer. However, this evidence is supported by in vitro or mouse model studies with no data from in vivo or human stud... Read More about Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs.

Intracellular mapping with SERS-encoded gold nanostars (2011)
Journal Article
Rodríguez-Lorenzo, L., Krpetic, Z., Barbosa, S., Alvarez-Puebla, R., Liz-Marzán, L., Prior, I., & Brust, M. (2011). Intracellular mapping with SERS-encoded gold nanostars. Integrative Biology, 3(9), 922-926. https://doi.org/10.1039/C1IB00029B

Here we report on the design, synthesis and application of small, highly bright, star-shaped SERS encoded single nanoparticles with the ability of providing an optical signal upon excitation with near infrared light. These particles are colloidally s... Read More about Intracellular mapping with SERS-encoded gold nanostars.

Acrylate-facilitated cellular uptake of gold nanoparticles (2011)
Journal Article
Krpetic, Z., Nativo, P., Prior, I., & Brust, M. (2011). Acrylate-facilitated cellular uptake of gold nanoparticles. Small, 7(14), 1982-1986. https://doi.org/10.1002/smll.201100462

Uptake of PEGylated gold nanoparticles by HeLa cells in the presence of acrylate is described. Nanoparticles that are usually not taken up readily cross the plasma membrane into the cytosol in the presence of acrylate. In addition, a method is descri... Read More about Acrylate-facilitated cellular uptake of gold nanoparticles.

Immunotherapy: what is its role in prostate cancer? (2011)
Journal Article
Elkord, E. (2011). Immunotherapy: what is its role in prostate cancer?

Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles (2011)
Journal Article
Krpeticc, Z., Saleemi, S., Prior, I., Sée, V., Qureshi, R., & Brust, M. (2011). Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles. ACS nano, 5(6), 5195-5201. https://doi.org/10.1021/nn201369k

This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. The TAT peptide... Read More about Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles.

Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure (2011)
Journal Article
Briston, S., Caldwell, J., Horn, M., Clarke, J., Richards, M., Greensmith, D., …Trafford, A. (2011). Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure. Journal of Physiology, 589(Pt 6), 1367-82. https://doi.org/10.1113/jphysiol.2010.203984

Reduced inotropic responsiveness is characteristic of heart failure (HF). This study determined the cellular Ca2+ homeostatic and molecular mechanisms causing the blunted β-adrenergic (β-AR) response in HF.We induced HF by tachypacing in sheep; intra... Read More about Impaired β-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure.

Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells (2011)
Thesis
Allsup, J. Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells. (Thesis). University of Salford

The aim of this project was to investigate the cellular biochemical and experimental therapeutic effects of L-glutamine (gin) and L-asparagine (asn) depletion for paediatric sarcoma cells. The work presented in this thesis describes preclinical stud... Read More about Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells.

Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats (2011)
Journal Article
Thomasson, D., Wright, E., Hughes, J., Dodd, N., Cox, A., Boyce, K., …Hide, G. (2011). Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats. Parasitology, 138(09), 1117-1123. https://doi.org/10.1017/S0031182011000904

The protozoan parasite Toxoplasma gondii is prevalent worldwide and can infect a remarkably wide range of hosts despite felids being the only definitive host. As cats play a major role in transmission to secondary mammalian hosts, the interaction b... Read More about Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats.