Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy (2008)
Journal Article
Elkord, E., Hawkins, R., & Stern, P. (2008). Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy. Expert Opinion on Biological Therapy, 8(4), 385-395. https://doi.org/10.1517/14712598.8.4.385

BACKGROUND: Despite improvement in conventional strategies for treating gastrointestinal (GI) carcinoma, large numbers of patients still suffer from incurable or progressive disease. OBJECTIVE: Here we consider the prospects for circumventing limi... Read More about Immunotherapy for gastrointestinal cancer: current status and strategies for improving efficacy.

Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia (2008)
Journal Article
Winters, U., Daayana, S., Lear, J., Tomlinson, A., Elkord, E., Stern, P., & Kitchener, H. (2008). Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia. Clinical Cancer Research, 14(16), 5292-5299. https://doi.org/10.1158/1078-0432.CCR-07-4760

PURPOSE: High-risk human papillomavirus (HPV)-associated vulval intraepithelial neoplasia (VIN) is difficult to treat by excision or ablation because of high recurrence rates. Small studies of photodynamic therapy (PDT) and imiquimod treatments have... Read More about Clinical and immunologic results of a phase II trial of sequential imiquimod and photodynamic therapy for vulval intraepithelial neoplasia.

Novel therapeutic strategies by regulatory T cells in allergy (2008)
Book Chapter
Elkord, E. (2008). Novel therapeutic strategies by regulatory T cells in allergy. In K. Blaser (Ed.), T Cell Regulation in Allergy, Asthma and Atopic Skin Diseases (150-157). Chemical Immunology and Allergy: Karger. https://doi.org/10.1159/000154999

Natural CD4+CD25+FoxP3+ regulatory T cells (Treg) actively suppress physiological and pathological responses, therefore playing a critical role in controlling peripheral tolerance to self antigens and maintaining immune homeostasis. In normal individ... Read More about Novel therapeutic strategies by regulatory T cells in allergy.

Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay (2008)
Journal Article
Bisby, R., Brooke, R., & Navaratnam, S. (2008). Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay. Food Chemistry, 108(3), 1002-1007. https://doi.org/10.1016/j.foodchem.2007.12.012

The "Oxygen Radical Absorption Capacity” (ORAC) assay (Ou, B., Hampsch-Woodill, M., Prior, R.L. (2001). Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultur... Read More about Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay.

Chemical composition and flavour development of cocoa products by thermal and enzymatic technologies (2008)
Thesis
Miralles-Garcia, J. Chemical composition and flavour development of cocoa products by thermal and enzymatic technologies. (Thesis). University of Salford

A novel technology to process traditional chocolate-making ingredients has been investigated with the main objective to promote the generation of key chocolate odorant volatiles as a part of the commercial interest of the industrial partner to create... Read More about Chemical composition and flavour development of cocoa products by thermal and enzymatic technologies.

Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation (2008)
Journal Article
Botchway, S., Parker, A., Bisby, R., & Crisostomo, A. (2008). Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation. Microscopy Research and Technique, 71(4), 267-273. https://doi.org/10.1002/jemt.20548

The real-time uptake of serotonin, a neurotransmitter, by rat leukaemia mast cell line RBL-2H3 and 5-hydroxytryptophan by Chinese hamster V79 cells has been studied by fluorescence lifetime imaging microscopy (FLIM), monitoring ultraviolet (340 nm) f... Read More about Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation.

The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria (2008)
Journal Article
Pages, J., James, C., & Winterhalter, M. (2008). The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria. Nature Reviews Microbiology, 6, 893-903. https://doi.org/10.1038/nrmicro1994

Gram-negative bacteria are responsible for a large proportion of antibiotic resistant bacterial diseases. These bacteria have a complex cell envelope that comprises an outer membrane and an inner membrane that delimit the periplasm. The outer membran... Read More about The porin and the permeating antibiotic: A selective diffusion barrier in gram-negative bacteria.

Circular dichroism to determine binding mode and affinity of ligand-DNA interactions (2007)
Journal Article
Garbett, N., Ragazzon, P., & Chaires, J. (2007). Circular dichroism to determine binding mode and affinity of ligand-DNA interactions. Nature Protocols, 2(12), 3166-3172

Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of in... Read More about Circular dichroism to determine binding mode and affinity of ligand-DNA interactions.

Use of competition dialysis in the discovery of G-quadruplex selective ligands (2007)
Journal Article
Ragazzon, P., & Chaires, J. (2007). Use of competition dialysis in the discovery of G-quadruplex selective ligands. Methods, 43(4), 313-323

G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand oreintations, to bimolecular dimeric structures, and finally to tetramolecular parallel-stranded... Read More about Use of competition dialysis in the discovery of G-quadruplex selective ligands.

Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria (2007)
Journal Article
Smith, D. L., James, C., Sergeant, M., Yaxian, Y., Saunders, J. R., McCarthy, A. J., & Allison, H. (2007). Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria. Journal of Bacteriology, 189, 7223-7233. https://doi.org/10.1128/JB.00824-07

Infection of Escherichia coli by Shiga toxin-encoding bacteriophages (Stx phages) was the pivotal event in the evolution of the deadly Shiga toxin-encoding E. coli (STEC), of which serotype O157:H7 is the most notorious. The number of different bacte... Read More about Short-tailed stx phages exploit the conserved YaeT protein to disseminate shiga toxin genes among enterobacteria.

Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. (2007)
Journal Article
Perez-Campo, F., Spencer, H., Elder, R., Stern, P., & Ward, C. (2007). Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. Experimental Cell Research, 313(16), 3604-15. https://doi.org/10.1016/j.yexcr.2007.07.021

The use of gene mutation/knock-out strategies in mouse embryonic stem (ES) cells has revolutionized the study of gene function in ES cells and embryonic development. However, the construction of vectors for homologous recombination strategies require... Read More about Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter..

Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya (2007)
Thesis
Gusbi, M. Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya. (Thesis). University of Salford

Two studies were performed to detect aetiology of acute diarrhoea in hospitalized children aged under five years in Tripoli, Libya. In the first study, two hundred and thirty-six stool samples were obtained. Of these, 118 were from patients admitted... Read More about Aetiology of acute diarrhoea in hospitalized children, Tripoli, Libya.

An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins (2007)
Journal Article
Dupont, M., James, C., Chevalier, J., & Pages, J. (2007). An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins. Antimicrobial Agents and Chemotherapy, 51(9), 3190-3198. https://doi.org/10.1128/AAC.01481-06

Bacterial adaptation to external stresses and toxic compounds is a key step in the emergence of multidrug-resistant strains that are a serious threat to human health. Although some of the proteins and regulators involved in antibiotic resistance mech... Read More about An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins.

Gold nanoparticles capped by peptides (2007)
Journal Article
Porta, F., Speranza, G., Krpetic, Z., Dal Santo, V., Francescato, P., & Scarì, G. (2007). Gold nanoparticles capped by peptides. Materials Science and Engineering: B, 140(3), 187-194. https://doi.org/10.1016/j.mseb.2007.03.019

Two dipeptides (GK and GC) and two 15-aminoacid peptides (GK15 and GC15) were used as capping agents in the preparation of monolayer-protected gold nanoparticles (MPCs). They were characterized by TEM microscopy, UV–vis, NMR and IR spectroscopy.

Competition dialysis: a method for the study of structural selective nucleic acid binding (2007)
Journal Article
Ragazzon, P., Garbett, N., & Chaires, J. (2007). Competition dialysis: a method for the study of structural selective nucleic acid binding. Methods, 42(2), 173-182. https://doi.org/10.1016/j.ymeth.2006.09.010

Competition dialysis is a powerful new tool for the discovery of ligands that bind to nucleic acids with structural- or sequence-selectivity. The method is based on firm thermodynamic principles and is simple to implement. In the competition dialysis... Read More about Competition dialysis: a method for the study of structural selective nucleic acid binding.

Unravelling the moulting degradome: new opportunities for chemotherapy? (2007)
Journal Article
Craig, H., Isaac, R., & Brooks, D. (2007). Unravelling the moulting degradome: new opportunities for chemotherapy?. Trends in Parasitology, 23(6), 248-253. https://doi.org/10.1016/j.pt.2007.04.003

Replacement of the nematode cuticle with a newly synthesized cuticle (a process known as moulting) occurs four times during larval development. Therefore, the key components of this essential developmental process represent attractive targets for new... Read More about Unravelling the moulting degradome: new opportunities for chemotherapy?.

Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. (2007)
Journal Article
Crisostomo, A., Moreno, R., Navaratnam, S., Wilkinson, J., & Bisby, R. (2007). Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. Free Radical Research, 41(6), 730-737. https://doi.org/10.1080/10715760701324075

Three potential routes to generation of reactive oxygen species from a tocopherolquinone have been identified. The quinone of the water-soluble vitamin E analogue Trolox C (Trol-Q) is reduced by hydrated electron and isopropanol a hydroxyalkyl radica... Read More about Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues..

Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold (2007)
Journal Article
Alcaro, S., Artese, A., Iley, J., Maccari, R., Missailidis, S., Ortuso, F., …Vigorita, M. (2007). Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold. Bioorganic and Medicinal Chemistry Letters, 1(17), 2509-2514

A series of fluorenone-carboxamide compounds was analyzed with regard to DNA binding properties by UV spectroscopy and competition dialysis methods. The morpholino derivative 10 provided interesting results in terms of affinity and specificity toward... Read More about Tetraplex DNA specific ligands based on the fluorenone-carboxamide scaffold.

Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus (2007)
Journal Article
to the laminated layer of echinococcus granulosus. Parasitology Research, 101(3), 647-652. https://doi.org/10.1007/s00436-007-0534-7

The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate... Read More about Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus.

Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection (2007)
Journal Article
Morgan, C., & Mitchell, A. (2007). Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection. Biosensors and Bioelectronics, 22(8), 1769-1775. https://doi.org/10.1016/j.bios.2006.08.024

Biological assays to detect binding interactions are often conducted using fluorescence resonance energy transfer (FRET) but this has several disadvantages that markedly reduce the dynamic range of measurements. The very short range of FRET interacti... Read More about Prospects for applications of lanthanide-based upconverting surfaces to bioassay and detection.