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Acrylate-facilitated cellular uptake of gold nanoparticles (2011)
Journal Article
Krpetic, Z., Nativo, P., Prior, I., & Brust, M. (2011). Acrylate-facilitated cellular uptake of gold nanoparticles. Small, 7(14), 1982-1986. https://doi.org/10.1002/smll.201100462

Uptake of PEGylated gold nanoparticles by HeLa cells in the presence of acrylate is described. Nanoparticles that are usually not taken up readily cross the plasma membrane into the cytosol in the presence of acrylate. In addition, a method is descri... Read More about Acrylate-facilitated cellular uptake of gold nanoparticles.

Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles (2011)
Journal Article
Krpeticc, Z., Saleemi, S., Prior, I., Sée, V., Qureshi, R., & Brust, M. (2011). Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles. ACS nano, 5(6), 5195-5201. https://doi.org/10.1021/nn201369k

This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. The TAT peptide... Read More about Negotiation of intracellular membrane barriers by TAT-Modified gold nanoparticles.

Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells (2011)
Thesis
Allsup, J. Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells. (Thesis). University of Salford

The aim of this project was to investigate the cellular biochemical and experimental therapeutic effects of L-glutamine (gin) and L-asparagine (asn) depletion for
paediatric sarcoma cells. The work presented in this thesis describes preclinical stud... Read More about Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells.

Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats (2011)
Journal Article

The protozoan parasite Toxoplasma gondii is prevalent worldwide and can infect a remarkably wide range of hosts despite
felids being the only definitive host. As cats play a major role in transmission to secondary mammalian hosts, the interaction
b... Read More about Prevalence and co-infection of Toxoplasma gondii and Neospora caninum in Apodemus sylvaticus in an area relatively free of cats.

Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status (2011)
Journal Article
Sawan, S., Burt, D., Stern, P., Holland, C., & Elkord, E. (2011). Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status. Immunological Investigations, 40(1), 62-75. https://doi.org/10.3109/08820139.2010.513022

Regulatory T cells (Treg) are a sub-population of T cells that suppress self-reactivity and are implicated in immune tolerance towards malignant cells. Circulating Treg cells are increased in several cancers. In endometrial cancer Treg cells have bee... Read More about Circulating regulatory T cells in endometrial cancer: a role for age and menopausal status.

Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells (2011)
Journal Article
Khan, S., Burt, D., Ralph, C., Hawkins, R., & Elkord, E. (2011). Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells. Clinical Immunology, 138(1), 85-96. https://doi.org/10.1016/j.clim.2010.09.011

Cytotoxic T Lymphocyte Antigen 4 (CTLA4) blockade has shown antitumor activity against common cancers. However, the exact mechanism of immune mediation by anti-CTLA4 remains to be elucidated. Further understanding of how CTLA4 blockade with tremelimu... Read More about Tremelimumab (anti-CTLA4) mediates immune responses mainly by direct activation of T effector cells rather than by affecting T regulatory cells.

Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells (2011)
Journal Article
Elkord, E., & Burt, D. (2011). Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells. Scandinavian Journal of Immunology, 74(4), 419-422. https://doi.org/10.1111/j.1365-3083.2011.02588.x

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical t... Read More about Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells.

The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa (2011)
Journal Article
Fothergill, J., Mowat, E., Walshaw, M., Ledson, M., James, C., & Winstanley, C. (2011). The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 55(1), 426-428. https://doi.org/10.1128/AAC.01257-10

Phage production in response to antibiotics varied among four isolates of a Pseudomonas aeruginosa
cystic fibrosis (CF) epidemic strain. Whereas ciprofloxacin induced higher levels of phage production,
other CF-relevant antibiotics led to reduced p... Read More about The effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.

Tubulin-binding Dibenz[c,e]oxepines as Colchinol analogues for targeting tumour vasculature (2010)
Journal Article
targeting tumour vasculature. Organic and Biomolecular Chemistry, 9(1), 219-231. https://doi.org/10.1039/c0ob00500b

Various methoxy- and hydroxy-substituted dibenz[c,e]oxepines were prepared via the
copper(I)-induced coupling of ether-tethered arylstannanes or the dehydrative cyclisation of 1,1'-
biphenyl-2,2'-dimethanols, assembled using the Ullmann cross-coupl... Read More about Tubulin-binding Dibenz[c,e]oxepines as Colchinol analogues for targeting tumour vasculature.

Inflicting controlled nonthermal damage to subcellular structures by laser-activated gold nanoparticles (2010)
Journal Article
Krpetic, Z., Nativo, P., Sée, V., Prior, I., Brust, M., & Volk, M. (2010). Inflicting controlled nonthermal damage to subcellular structures by laser-activated gold nanoparticles. Nano Letters, 10(11), 4549-4554. https://doi.org/10.1021/nl103142t

We show that low-intensity laser irradiation of cancer cells containing endosomal gold nanoparticles leads to endosome rupture and escape of the nanoparticles into the cytosol without affecting the cells’ viability. The low light intensity of our exp... Read More about Inflicting controlled nonthermal damage to subcellular structures by laser-activated gold nanoparticles.

Phagocytosis of biocompatible gold nanoparticles (2010)
Journal Article
Krpeticc, Z., Porta, F., Caneva, E., Dal Santo, V., & Scarì, G. (2010). Phagocytosis of biocompatible gold nanoparticles. Langmuir, 26(18), 14799-14805. https://doi.org/10.1021/la102758f

We report the evidence for the cellular uptake of gold nanoparticles via the phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules of choi... Read More about Phagocytosis of biocompatible gold nanoparticles.

Isolation of 1,4-Li2-C6H4 and its reaction with [(Ph3P)AuCl] (2010)
Journal Article
Flower, K., Mcgown, A., Miles, P., Pritchard, R., & Warren, J. (2010). Isolation of 1,4-Li2-C6H4 and its reaction with [(Ph3P)AuCl]. Dalton Transactions, 39(14), 3509-3520. https://doi.org/10.1039/b925355f

The difficulty in generating 1,4-Li2-C6H4 utilising the lithium halogen exchange reaction on 1,4-Br2-C6H4, 1,4-I2-C6H4 and 1-Br-4-I-C6H4 is revisited and only on treatment of 1,4-I2-C6H4 with 2 molar equivalents of n-BuLi can 1,4-Li2-C6H4 1 be isolat... Read More about Isolation of 1,4-Li2-C6H4 and its reaction with [(Ph3P)AuCl].

Cross-resistance of Cry1Ab-selected Asian corn borer to other Cry toxins (2010)
Journal Article
Xu, L., Wang, Z., Zhang, J., Ferry, N., & Gatehouse, A. (2010). Cross-resistance of Cry1Ab-selected Asian corn borer to other Cry toxins. Journal of Applied Entomology, 134(5), 429-438. https://doi.org/10.1111/j.1439-0418.2010.01517.x

The Asian corn borer, Ostrinia furnacalis (Guene´e) (Lepidoptera: Crambidae), is the most important insect pest of maize in China. Despite the proven track record of biotech Bacillus thuringiensis (Bt) maize to provide an effective means to control l... Read More about Cross-resistance of Cry1Ab-selected Asian corn borer to other Cry toxins.

Phase phenomena in supported lipid films under varying electric potential (2010)
Journal Article
Brukhno, A., Akinshina, A., Coldrick, Z., Nelson, A., & Auer, S. (2011). Phase phenomena in supported lipid films under varying electric potential. Soft Matter, 7(3), 1006-1017. https://doi.org/10.1039/C0SM00724B

We model cyclic voltammetry experiments on supported lipid films where a non-trivial dependence of the capacitance on the applied voltage is observed. Previously, based on a mean-field treatment of the Flory–Huggins type, under the assumption of stro... Read More about Phase phenomena in supported lipid films under varying electric potential.

The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells (2010)
Journal Article
Cooley, N., Elder, R., & Povey, A. (2010). The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells. Toxicology, 268(1-2), 111-117. https://doi.org/10.1016/j.tox.2009.12.008

The DNA structure recognition protein MSH2 is an important protein in DNA mismatch repair due to its role in initiating the repair process. To examine the potential interactions between mismatch repair and base excision repair (BER) we have examined... Read More about The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells.

Investigation of the effects of schistosoma mansoni eggs on hepatic stellate cell phenotype (2010)
Thesis
Anthony, B. Investigation of the effects of schistosoma mansoni eggs on hepatic stellate cell phenotype. (Thesis). Salford : University of Salford

Schistosomiasis is one of the most important helminth infections in man,
infecting an estimated 200 million people worldwide leaving 20 million people
with severe morbidity and causing 20,000 deaths a year. Of chronically
infected patients approxi... Read More about Investigation of the effects of schistosoma mansoni eggs on hepatic stellate cell phenotype.

T regulatory cells in cancer: recent advances and therapeutic potential (2010)
Journal Article
Elkord, E., Alcantar-Orozco, E., Dovedi, S., & Tran, D. (2010). T regulatory cells in cancer: recent advances and therapeutic potential. Expert Opinion on Biological Therapy, 10(11), 1573-1586. https://doi.org/10.1517/14712598.2010.529126

IMPORTANCE OF THE FIELD: The active suppression of immune responses against tumor is a major barrier to the likely success of cancer immunotherapy. There is now compelling evidence implicating T regulatory cells (Tregs) as being key players driving i... Read More about T regulatory cells in cancer: recent advances and therapeutic potential.

A riot of rhythms : neuronal and glial circadian oscillators in the mediobasal hypothalamus (2009)
Journal Article
Guilding, C., Hughes, A., Brown, T., Namvar, S., & Piggins, H. (2009). A riot of rhythms : neuronal and glial circadian oscillators in the mediobasal hypothalamus. Molecular Brain, 2(28), https://doi.org/10.1186/1756-6606-2-28

Background: In mammals, the synchronized activity of cell autonomous clocks in the suprachiasmatic nuclei (SCN) enables this structure to function as the master circadian clock, coordinating daily rhythms in physiology and behavior. However, the domi... Read More about A riot of rhythms : neuronal and glial circadian oscillators in the mediobasal hypothalamus.